Evidence for Genetic Linkage between the Gene Segments Encoding NSP4 and VP6 Proteins in Common and Reassortant Human Rotavirus Strains

Iturriza‐Gómara, Miren; Anderton, Emma; Kang, Gagandeep; Gallimore, Christopher; Phillips, W. M.; Desselberger, Ulrich; Gray, Jim

doi:10.1128/jcm.41.8.3566-3573.2003

Cited by 75 publications

(75 citation statements)

References 38 publications

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“…NSP4 genotypes have been shown to segregate according to their species of origin suggesting distinct evolutionary patterns of circulating rotaviruses [Ciarlet et al, 2000]. Additionally, linkage has been demonstrated between NSP4 genotype and VP6 subgroup in HRVs by analysis of a range of common and reassortant strains, including 38 strains from Vellore, where genes encoding SGI always co-segregate with NSP4-A, and SGII with NSP4-B [Iturriza-Gomara et al, 2003]. The G12 strains from Vellore belonged to VP6 SGII and NSP4 B genotype, which is consistent with our previous findings.…”

Section: Discussionmentioning

confidence: 99%

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Modification of rotavirus multiplex RT‐PCR for the detection of G12 strains based on characterization of emerging G12 rotavirus strains from South India

Banerjee¹,

Ramani²,

Primrose³

et al. 2007

Journal of Medical Virology

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101

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Rotaviruses are the major etiological agents of diarrhea in children less than 5 years of age. The commonest G types in humans are G1-4 and G9. G12 is a rare human rotavirus (HRV) strain first reported in the Philippines. In this study, 13 G12 strains obtained from a community-based cohort and a hospital-based surveillance system in 2005 were characterized by phylogenetic analysis of partial nucleotide sequences of VP7, VP6, and NSP4 genes. Sequence and phylogenetic analysis of VP7 gene sequences showed that these southern Indian strains had the greatest homology with G12 strains recently reported from eastern India (97-99% identity both at the nucleotide level and deduced amino acid level) and less homology with the prototype G12 strain, L26 (89-90% identity at the nucleotide level and 90-94% at the deduced amino acid level). Phylogenetic analysis of the VP6 and the NSP4 genes revealed that the Vellore G12 strains belonged to VP6 subgroup II and NSP4 genotype B. The P types associated with these strains were P[6] and P [8]. A G12 typespecific primer was designed for inclusion in an established VP7 G-typing multiplex RT PCR, and tested against a panel of known G types and untyped samples and was found to detect G12 strains in the multiplex-PCR. Close homology of the South Indian G12 strains to those from Kolkata suggests that G12 HRV strains are emerging in India. Methods for characterization of rotaviruses in epidemiological studies need to be updated frequently, particularly in developing countries.

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Section: Discussionmentioning

confidence: 99%

“…Analysis of the gene encoding NSP4 of various animal and HRV strains has identified five genotypes (A-E) [Ciarlet et al, 2000;Iturriza-Gomara et al, 2003]. As seen with the VP6 gene, the strains cluster within genotypes according to their species of origin [Ciarlet et al, 2000].…”

Section: Introductionmentioning

confidence: 99%

Modification of rotavirus multiplex RT‐PCR for the detection of G12 strains based on characterization of emerging G12 rotavirus strains from South India

Banerjee¹,

Ramani²,

Primrose³

et al. 2007

Journal of Medical Virology

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101

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“…Rotavirus subgroups (VP6) and NSP4 genotypes were determined for uncommon or novel strains identified through Gand P-typing in order to identify possible zoonotic transmission [18,19] (European rotavirus detection and characterization methods v4).…”

Section: Rotavirus Genotypingmentioning

confidence: 99%

Rotavirus genotypes co-circulating in Europe between 2006 and 2009 as determined by EuroRotaNet, a pan-European collaborative strain surveillance network

Iturriza‐Gómara¹,

Dallman²,

Bányai³

et al. 2010

Epidemiol. Infect.

205

254

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SUMMARYEuroRotaNet, a laboratory network, was established in order to determine the diversity of co-circulating rotavirus strains in Europe over three or more rotavirus seasons from 2006/2007 and currently includes 16 countries. This report highlights the tremendous diversity of rotavirus strains co-circulating in the European population during three years of surveillance since 2006/ 2007 and points to the possible origins of these strains including genetic reassortment and interspecies transmission. Furthermore, the ability of the network to identify strains circulating with an incidence of o1% allowed the identification of possible emerging strains such as G8 and G12 since the beginning of the study ; analysis of recent data indicates their increased incidence.

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“…Human RVA can be divided into three major genome constellations (GI-GIII), being the Wa strain as the prototype for GI, DS-1 for GII and AU-1 for GIII. This strong association of genes (genome constellations) has been explained by specific interactions of the viral proteins (Benati et al, 2010;Heiman et al, 2008;Iturriza-Gò mara et al, 2003;McDonald et al, 2009). Thus, genes from one specific genome constellation appear to co-segregate, restricting reassortment amongst different genotypes.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, viral protein interactions might drive the selection of strains that can replicate and spread better in different species (Iturriza-Gò mara et al, 2003;Santos et al, 2003). In this study we characterized the VP4 (VP8 * ), VP6 and VP7 genes from a large sample collection of equine RVA strains.…”

Section: Introductionmentioning

confidence: 99%

Genetic linkage of capsid protein-encoding RNA segments in group A equine rotaviruses

Miño¹,

Barrandeguy

Parreño³

et al. 2016

Journal of General Virology

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Rotavirus virions are formed by three concentric protein layers that enclose the 11 dsRNA genome segments and the viral proteins VP1 and VP3. Interactions amongst the capsid proteins (VP2, VP6, VP7 and VP4) have been described to play a major role in viral fitness, whilst restricting the reassortment of the genomic segments during co-infection with different rotavirus strains. In this work we describe and characterize the linkage between VP6 and VP7 proteins based on structural and genomic analyses of group A rotavirus strains circulating in Argentinean horses. Strains with the VP7 genotype G3 showed a strong association with the VP6 genotype I6, whilst strains with G14 were associated with the I2 genotype. Most of the differences on the VP6 and VP7 proteins were observed in interactive regions between the two proteins, suggesting that VP6 : VP7 interactions may drive the co-evolution and co-segregation of their respective gene segments.

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Evidence for Genetic Linkage between the Gene Segments Encoding NSP4 and VP6 Proteins in Common and Reassortant Human Rotavirus Strains

Cited by 75 publications

References 38 publications

Modification of rotavirus multiplex RT‐PCR for the detection of G12 strains based on characterization of emerging G12 rotavirus strains from South India

Modification of rotavirus multiplex RT‐PCR for the detection of G12 strains based on characterization of emerging G12 rotavirus strains from South India

Rotavirus genotypes co-circulating in Europe between 2006 and 2009 as determined by EuroRotaNet, a pan-European collaborative strain surveillance network

Genetic linkage of capsid protein-encoding RNA segments in group A equine rotaviruses

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