Evidence for ‘cross–talk’ between A and B chromosomes of rye

Ribeiro, Teresa; Pires, Bruno; Delgado, Margarida; Viegas, Wanda; Jones, Neil; Morais-Cecílio, Leonor

doi:10.1098/rsbl.2004.0228

Cited by 10 publications

(6 citation statements)

References 11 publications

(15 reference statements)

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“…Seeds of a rye (Secale cereale L.) self-fertile inbred line 7415 with and without Bs (Jimenez et al, 1994), hexaploid wheat (Triticum aestivum L.) 'Lindstr€ om' with and without added standard rye Bs (Lindstr€ om, 1965) and rye JBK lines with truncated Bs (lacking the terminal part of the long arm) (Ribeiro et al, 2004) were germinated on wet filter papers. Plant cultivation conditions are described in Supporting Information Methods S1.…”

Section: Plant Materials and Cultivationmentioning

confidence: 99%

Rye B chromosomes encode a functional Argonaute‐like protein with in vitro slicer activities similar to its A chromosome paralog

Gabriel

Martis

et al. 2016

New Phytologist

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B chromosomes (Bs) are supernumerary, dispensable parts of the nuclear genome, which appear in many different species of eukaryote. So far, Bs have been considered to be genetically inert elements without any functional genes. Our comparative transcriptome analysis and the detection of active RNA polymerase II (RNAPII) in the proximity of B chromatin demonstrate that the Bs of rye (Secale cereale) contribute to the transcriptome. In total, 1954 and 1218 B-derived transcripts with an open reading frame were expressed in generative and vegetative tissues, respectively. In addition to B-derived transposable element transcripts, a high percentage of short transcripts without detectable similarity to known proteins and gene fragments from A chromosomes (As) were found, suggesting an ongoing gene erosion process. In vitro analysis of the A- and B-encoded AGO4B protein variants demonstrated that both possess RNA slicer activity. These data demonstrate unambiguously the presence of a functional AGO4B gene on Bs and that these Bs carry both functional protein coding genes and pseudogene copies. Thus, B-encoded genes may provide an additional level of gene control and complexity in combination with their related A-located genes. Hence, physiological effects, associated with the presence of Bs, may partly be explained by the activity of B-located (pseudo)genes.

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Section: Plant Materials and Cultivationmentioning

confidence: 99%

Rye B chromosomes encode a functional Argonaute‐like protein with in vitro slicer activities similar to its A chromosome paralog

Gabriel

Martis

et al. 2016

New Phytologist

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“…First, there is a positive correlation between genome size and the number of B chromosomes (Ayonoadu and Rees, 1971;Jones and Rees, 1982;Rosato et al, 1998;Delgado et al, 2004); by contrast, the genome size of plants with B chromosomes may in some cases be even smaller that those with no B chromosomes (Porter and Rayburn, 1990;Poggio et al, 1998). B chromosomes are, moreover, widely accepted to be derived from Achromosomes (Jones and Houben, 2003, and references therein;Leach et al, 2005) and interchanges with normal A-chromosomes have also been documented (Nokkala and Nokkala, 2004;Ribeiro et al, 2004).…”

Section: B Chromosomesmentioning

confidence: 99%

Intraspecific DNA Content Variability in Festuca pallens on Different Geographical Scales and Ploidy Levels

Šmarda

Bureš

2006

Annals of Botany

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Genome size variability occurs over all spatial scales: intrapopulation, landscape and global. Correlation between geographical coordinates and palaeovegetation type, concomitant with diploids and tetraploids, and no influence of microhabitat were found. Genome size decreases in tetraploids. Lower CVs, and thus higher accuracy, resolution and reproducibility, favour DAPI measurements for the study of intraspecific genome size variability.

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“…Seeds of rye (Secale cereale cv JNK; 2n ¼ 2x ¼ 14 þ Bs) and wheat (Triticum aestivum cv Lindströ m; 2n ¼ 6x ¼ 42 þ Bs), both known to carry rye Bs, and a JNK rye line selected for the presence of deleted Bs (structural variants that lack the terminal part of the long arm (Ribeiro et al, 2004), were germinated on moist filter paper at 248C.…”

Section: Plant Materialsmentioning

confidence: 99%

Transcriptionally Active Heterochromatin in Rye B Chromosomes

et al. 2007

Self Cite

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B chromosomes (Bs) are dispensable components of the genomes of numerous species. Thus far, there is a lack of evidence for any transcripts of Bs in plants, with the exception of some rDNA sequences. Here, we show that the Giemsa banding-positive heterochromatic subterminal domain of rye (Secale cereale) Bs undergoes decondensation during interphase. Contrary to the heterochromatic regions of A chromosomes, this domain is simultaneously marked by trimethylated H3K4 and by trimethylated H3K27, an unusual combination of apparently conflicting histone modifications. Notably, both types of B-specific high copy repeat families (E3900 and D1100) of the subterminal domain are transcriptionally active, although with different tissue type–dependent activity. No small RNAs were detected specifically for the presence of Bs. The lack of any significant open reading frame and the highly heterogeneous size of mainly polyadenylated transcripts indicate that the noncoding RNA may function as structural or catalytic RNA.

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Evidence for ‘cross–talk’ between A and B chromosomes of rye

Cited by 10 publications

References 11 publications

Rye B chromosomes encode a functional Argonaute‐like protein with in vitro slicer activities similar to its A chromosome paralog

Rye B chromosomes encode a functional Argonaute‐like protein with in vitro slicer activities similar to its A chromosome paralog

Intraspecific DNA Content Variability in Festuca pallens on Different Geographical Scales and Ploidy Levels

Transcriptionally Active Heterochromatin in Rye B Chromosomes

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