The normal state resistivity of single phase polycrystalline Gd(Ba 2-x Pr x )Cu 3 O 7+δ samples with 0.0 ≤ x ≤ 0.50 have been investigated. The two-dimensional variable range hopping is dominant in the normal state resistivity of the samples. The conduction shows that Pr-doping strongly localizes the carriers in normal state, and finally causes the suppression of superconductivity. Based on the resistivity measurements, the effect of Pr substitution in 123 structure of HTSC at Gd or Ba site is to increase the pseudogap temperature T s , although, Pr at Ba site has a stronger effect on the suppression of T s and superconductivity. Prdoping not only reduces the carrier density and induces pseudogap, but also simultaneously increases T s and suppresses the linearity behavior of resistivity vs. temperature in the normal state.1 Introduction The normal state of the high temperature cuprate superconductors shows many unusual properties, which are far from the standard Fermi liquid behavior. An example is the so-called pseudogap behavior observed in the under-doped region near the Mott insulating phase, which is a frequency threshold for the strong excitation of spin and charge modes [1]. Among different models for describing the charge transport in materials, hopping conduction between localized states have been widely used for normal state of HTSC, semiconductors, and perovskites. In hopping conduction, the temperature dependence of resistivity is ρ = ρ 0 (T/T 0 ) 2p exp(T 0 /T) p , where T 0 is a characteristic temperature, which will be discussed later, and p = 1/3 in two dimensions (2D), p=1/4 in three dimensions (3D), and p = 1/2 in Coulomb gap (CG) regime [2]. The CG, 2D-VRH, and 3D-VRH have been reported for normal state resistivity of different HTSCs [3].The pseudogap has been observed from different experiments. In addition, due to the extremely complex structural and electronic nature of the high T c cuprates [4], and the somewhat controversial nature of much of the current experimental data, there are many theoretical attempts to explain the pseudogap behavior. A number of families of HTSC have also shown evidences of pseudogap [5]. In this paper, through resistivity measurements, we will investigate the effect of Pr at Ba and R sites in GdBa 2 Cu 3 O 7 -based compound from the conduction mechanism and pseudogap points of view.