Evidence For Autogenous Regulation of
            <i>Pseudomonas putida</i>
            Tryptophan Synthase

Proctor, Alan R.; Crawford, Irving P.

doi:10.1128/jb.126.1.547-549.1976

Cited by 8 publications

(1 citation statement)

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“…trp mutants of P. fluorescens 6.2 were recognized by Irving Crawford (personal communication) as exhibiting a typical Pseudomonas regulation. It included repressibility of trpE, -D and -C genes, constitutivity of the trpF gene, and inducibility of trpA and -B genes (10,17,31,32). Six tip mutants were tested in complementation attempts with F123 and F125 plasmids.…”

Section: Resultsmentioning

confidence: 99%

F'-plasmid transfer from Escherichia coli to Pseudomonas fluorescens

Mergeay¹,

Gerits²

1978

J Bacteriol

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Various F' plasmids of Escherichia coli K-12 could be transferred into mutants of the soil strain 6.2, classified herein as a Pseudomonas fluorescens biotype IV. This strain was previously found to receive Flac plasmid (N. Datta and R. W. Hedges, J. Gen. Microbiol. 70:453-460, 1972). ilv, leu, met, arg, and his auxotrophs were complemented by plasmids carrying isofunctional genes; trp mutants were not complemented or were very poorly complemented. The frequency of transfer was 10-5. Subsequent transfer into other P. fluorescens recipients was of the same order of magnitude. Some transconjugants were unable to act as donors, and these did not lose the received information if subcultured on nonselective media. Use of F' plasmids helped to discriminate metabolic blocks in P. fluorescens. In particular, metA, metB, and argH mutants were so distinguished. In addition, F131 plasmid carrying the his operon and a supD mutation could partially relieve the auxotrophy of thr, ilv, and metA13 mutants, suggesting functional expression of E. coli tRNA in P. fluorescens. In P. fluorescens metA Rifr mutants carrying the F110 plasmid, which carried the E. coli metA gene and the E. coli rif8 allele, sensitivity to rifampin was found to be dominant at least temporarily over resistance. This suggests interaction of E. coli and P. fluorescens subunits of RNA polymerase. his mutations were also complemented by composite P plasmids containing the his-nif region of Klebsiella pneumoniae (plasmids FN68 and RP41). nif expression could be detected by acetylene reduction in some his' transconjugants. The frequency of transfer of these P plasmids was 5 x 1O-'.

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Section: Resultsmentioning

confidence: 99%