Autogenous and Classical Regulation of Gene Expression: A General Theory and Experimental Evidence

Genetic analysis of a temperature-sensitive Salmonella typhimurium rho mutant with an altered rho-associated polycytidylate-dependent adenosine triphosphatase activity

“…Strains diploid for the structural gene (ompA) of a major outer cell envelope membrane protein of E. coli do not express a twofold increase in the level of the ompA protein (10). Expression of the ompA gene may be autogenously regulated (34). In the present work, there was no gene dosage effect in strains diploid for the wild-type rho allele, and the mutant strain contained significantly elevated levels of poly(C)-dependent ATPase activity.…”

Subunit Structure of Regulator Proteins Influences the Design of Gene Circuitry: Analysis of Perfectly Coupled and Completely Uncoupled Circuits

“…The experimental evidence for a few circuits with the negative mode of regulation was in agreement with the prediction of a perfectly coupled circuit. In other well-studied cases, like that of the lac operon, the evidence clearly suggested completely uncoupled rather than perfectly coupled circuits (Savageau, 1979). Thus, experimental data available at the time provided some support for the predictions of this circuit theory; however, there were obvious exceptions like the lac operon.…”

“…The results allowed us to predict classical circuits when the regulator is an activator and autogenous circuits when the regulator is a repressor. These predictions have been supported in a number of cases for which the experimental data clearly indicate classical or autogenous circuits (Savageau, 1979). However, there also are notable exceptions like the lac operon of E. coli, which has a classical circuit while the regulator is a repressor.…”

“…These criteria apply to inducible systems of other types, although the interpretation of the criteria may be slightly different (e.g. see Savageau, 1979). The 2-dimensional space of regulatory parameters (g12 and g13) for completely uncoupled circuits and perfectly coupled circuits.…”

Completely uncoupled and perfectly coupled gene expression in repressible systems 1 1Edited by K. Yamamoto

“…Although these criteria represent distinct properties chosen on the basis of our knowledge of cell physiology, their manifestation may or may not vary in a consistent pattern from one system to another. Our criteria are stated speci®cally for biosynthetic systems, although they are perhaps more widely applicable (Savageau, 1979).…”