1984
DOI: 10.1111/j.1432-1033.1984.tb08347.x
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Evidence for a structural relationship between apoB75 kDa and human plasma apolipoprotein B100, from translation of human liver mRNA in vitro and immunochemical studies with monoclonal and polyclonal antibodies

Abstract: We have investigated the relation between an XO-kDa protein synthesized in vitro in protein-synthesizing system programmed with human liver mRNA [Olofsson, S.-O., Elias, P., Bostrom, K., Lundholm, K., Norfeldt, P.-I., Wiklund, O., Fager, G., and Bondjers, G. (1983) Five monoclonal antibodies directed against LDL-2 as well as polyclonal antibodies against a narrow density cut of LDL-2 (d= 1.030 -1.055) were used to precipitate apoB-related proteins synthesized in vitro in a proteinsynthesizing system programme… Show more

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Cited by 12 publications
(9 citation statements)
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“…All antibodies used to precipitate apoB proteins from cell lysate or from culture medium were purified by adsorption chromatography as described earlier [20]. Immunoglobulins were isolated by ammonium sulphate precipitation [20].…”
Section: Preparation Of Antibodiesmentioning
confidence: 99%
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“…All antibodies used to precipitate apoB proteins from cell lysate or from culture medium were purified by adsorption chromatography as described earlier [20]. Immunoglobulins were isolated by ammonium sulphate precipitation [20].…”
Section: Preparation Of Antibodiesmentioning
confidence: 99%
“…For immunoprecipitation with monoclonal antibodies the samples were incubated for 4 h at room temperature with 100 Fg of non-immune mouse antibodies (in buffer B) (20), 150 pg of rabbit antibodies to mouse immunoglobulins (in buffer B) was then added and the incubation was continued for 2 h. Immunoprecipitin (0.2 ml of buffer C) was added and the sample was incubated for an additional hour. The Immunoprecipitin pellet was treated as described for the precipitation with polyclonal antibodies (see above).…”
Section: Immunoprecipitation Of Apobmentioning
confidence: 99%
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