1985
DOI: 10.1111/j.1432-1033.1985.tb08947.x
|View full text |Cite
|
Sign up to set email alerts
|

Pulse‐chase studies of the synthesis of apolipoprotein B in a human hepatoma cell line, Hep G2

Abstract: We have used pulse-chase methodology to study the synthesis of apolipoprotein B in a human hepatomaderived cell line, the Hep G2 cells. A 2-min pulse with [35S]methionine was followed by a chase period varying from 5-90 min. A protein of large molecular mass (estimated molecular mass: 312 f 41 kDa, mean & SD, n = 8) could be immunoprecipitated from the cells at all chase periods between 5 min and 60 min with both monoclonal antibodies to a narrow density cut of the low density lipoprotein LDL-2 (density: 1.030… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
28
0

Year Published

1987
1987
2012
2012

Publication Types

Select...
10

Relationship

2
8

Authors

Journals

citations
Cited by 49 publications
(28 citation statements)
references
References 34 publications
0
28
0
Order By: Relevance
“…Electrophoresis in SDS-polyacrylamide gels, autoradiography, and determination of the radioactivity in the proteins separated in the gels was carried out as described (29). The culture medium was recovered after the 120-min chase and the secretion of pulse-labeled transferrin was determined.…”
Section: Methodsmentioning
confidence: 99%
“…Electrophoresis in SDS-polyacrylamide gels, autoradiography, and determination of the radioactivity in the proteins separated in the gels was carried out as described (29). The culture medium was recovered after the 120-min chase and the secretion of pulse-labeled transferrin was determined.…”
Section: Methodsmentioning
confidence: 99%
“…There is a temporal delay in the transfer from the ER, which is regulated by the microsomal membrane association of proteins. Albumin, haptoglobin, and transferrin have a half-life of secretion of 30, 40, and 75 min, respectively (41), and it takes ϳ30 min for newly synthesized ApoB 100 to be packaged and released into the circulation (42,43); thus there is a time lag between protein synthesis and appearance in the plasma.…”
Section: Plasma Proteome Dynamicsmentioning
confidence: 99%
“…Precipitates from all fractions were analyzed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) followed by autoradiography (14). ApoB-100 was present in three well separated fractions: the upper 3 ml of the gradient (d Ͻ 1.006 g/ml, corresponding to VLDL), the middle of the gradient (d ϭ 1.006 -1.06 g/ml), and the bottom of the gradient (d ϭ 1.06 -1.21 g/ml).…”
Section: S]methionine and L-[mentioning
confidence: 99%