Evidence for a nitrate-independent function of the nitrate sensor NRT1.1 in Arabidopsis thaliana

Hachiya, Takushi; Mizokami, Yusuke; Tholen, Danny; Watanabe, Chihiro; Noguchi, Ko

doi:10.1007/s10265-010-0385-7

Cited by 46 publications

(54 citation statements)

References 29 publications

(44 reference statements)

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“…Recently, we have revealed that knockout mutants of NRT1.1 show enhanced tolerance to ammonium and/or low pH conditions in the absence of nitrate. 3 This observation directly suggests the existence of a nitrateindependent function of NRT1.1. In this addendum, putative mechanisms for this observation are discussed and prospective experimental approaches are suggested as below.…”

mentioning

confidence: 79%

“…3 Recently, some components related to the primary nitrate response have been determined. 8 Therefore, altered responses of these components may be related to the ammonium/low pH tolerance in the NRT1.1 mutants, although chl1-9, which has a normal primary nitrate response at least via NRT1.1, shows the tolerance.…”

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confidence: 99%

“…effects of auxin application on the ammonium/low pH tolerance of chl1-5 (c). nO 3 -and nH 4 + denote 10 mM of nitrate and ammonium as n sources, respectively. In one dish, nine seeds (a and B) or 18 seeds (c) of each line were placed and grown for eleven days.…”

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confidence: 99%

“…In one dish, nine seeds (a and B) or 18 seeds (c) of each line were placed and grown for eleven days. Other detail growth conditions are shown in reference 3. at the time of sampling, the pH of growth media were 6.2-6.4 in nO 3 -medium and 4.1-4.3 in nH 4 + medium. Means and standard errors of shoot FW and chl concentration were determined regarding mean value of one dish as one biological replicase (n ≥ 3).…”

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confidence: 99%

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Mutation of NRT1.1 enhances ammonium/low pH-tolerance in Arabiopsis thaliana

Hachiya

Noguchi

2011

Plant Signaling & Behavior

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confidence: 79%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Mutation of NRT1.1 enhances ammonium/low pH-tolerance in Arabiopsis thaliana

Hachiya

Noguchi

2011

Plant Signaling & Behavior

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“…If correct, this model implies that manipulation of the nutrient sensing systems in the roots will affect the response of shoot growth to changes in nutrient availability. Interestingly, the NRT1.1 NO 3 − transceptor, which participates in the NO 3 − regulation of LR growth in A. thaliana (see above section), has recently been shown to also modulate shoot growth as a function of the N source (Hachiya et al 2011). However, there is no evidence yet that a putative role of NRT1.1 in the regulation of shoot growth is due to modified root-to-shoot CK signalling.…”

Section: Slowing Down Of Shoot Growthmentioning

confidence: 99%

Nitrogen acquisition by roots: physiological and developmental mechanisms ensuring plant adaptation to a fluctuating resource

2013

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Background Nitrogen (N) is one of the key mineral nutrients for plants and its availability has a major impact on their growth and development. Most often N resources are limiting and plants have evolved various strategies to modulate their root uptake capacity to compensate for both spatial and temporal changes in N availability in soil. The main N sources for terrestrial plants in soils of temperate regions are in decreasing order of abundance, nitrate, ammonium and amino acids. N uptake systems combine, for these different N forms, high-and low-affinity transporters belonging to multige families. Expression and activity of most uptake systems are regulated locally by the concentration of their substrate, and by a systemic feedback control exerted by whole-plant signals of N status, giving rise to a complex combinatory network. Besides modulation of the capacity of transport systems, plants are also able to modulate their growth and development to maintain N homeostasis. In particular, root system architecture is highly plastic and its changes can greatly impact N acquisition from soil.Scope In this review, we aim at detailing recent advances in the identification of molecular mechanisms responsible for physiological and developmental responses of root N acquisition to changes in N availability. These mechanisms are now unravelled at an increasing rate, especially in the model plant Arabidopsis thaliana L.. Within the past decade, most root membrane transport proteins that determine N acquisition have been identified. More recently, molecular regulators in nitrate or ammonium sensing and signalling have been isolated, revealing common regulatory genes for transport system and root development, as well as a strong connection between N and hormone signalling pathways. Conclusion Deciphering the complexity of the regulatory networks that control N uptake, metabolism and plant development will help understanding adaptation of plants to sub-optimal N availability and fluctuating environments. It will also provide solutions for addressing the major issues of pollution and economical costs related to N fertilizer use that threaten agricultural and ecological sustainability.

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The nitrate transporter NRT1.1 is involved in iron deficiency responses in Arabidopsis

Liu

Cui

et al. 2015

J. Plant Nutr. Soil Sci.

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Iron (Fe) deficiency strongly induces Fe-acquisition gene expression. The nitrate transporter NRT1.1, which functions as a dual-affinity nitrate transporter and nitrate signaling sensor, is involved in many nitrate-dependent responses. However, the regulation of plant responses to Fe deficiency by NRT1.1 has not been described. In this study, it is shown that NRT1.1 is downregulated by Fe deficiency. The functional disruption of NRT1.1 enhanced resistance to Fe deficiency stress: after 10 d Fe starvation, the shoot chlorosis phenotype of the NRT1.1-deficient mutant chl1-5 was alleviated compared to the wild type (WT). Furthermore, the levels of IRT1, FRO2, and FIT transcripts were less induced in chl1-5 plants under Fe-deficient conditions. Less nitrate accumulated in chl1-5 plants compared to WT and nitrate reductase (NR) activity was significantly decreased in WT plants after 10 d Fe deficiency treatment but not in the mutant. We propose that the functional disruption of NRT1.1 helps Arabidopsis plants to better adapt to Fe deficiency stress, which is most likely explained in terms of a physiological mechanism by which the reduced amount of accumulated internal nitrate could to impair an FIT-dependent Fe deficiency signaling pathway via a feedback mechanism.

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Evidence for a nitrate-independent function of the nitrate sensor NRT1.1 in Arabidopsis thaliana

Cited by 46 publications

References 29 publications

Mutation of NRT1.1 enhances ammonium/low pH-tolerance in Arabiopsis thaliana

Mutation of NRT1.1 enhances ammonium/low pH-tolerance in Arabiopsis thaliana

Nitrogen acquisition by roots: physiological and developmental mechanisms ensuring plant adaptation to a fluctuating resource

The nitrate transporter NRT1.1 is involved in iron deficiency responses in Arabidopsis

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