The nitrate transporter NRT1.1 is involved in iron deficiency responses in <i>Arabidopsis</i>

Liu, Xunyan; Cui, Haoqiang; Li, Anna; Zhang, Ming; Teng, Yibo

doi:10.1002/jpln.201400480

Cited by 13 publications

(13 citation statements)

References 53 publications

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“…In our hydroponic system, we showed that Fe deficiency markedly downregulated the level of NRT1.1-GFP in both the shoots and roots of plants, but did not affect the gene expression of NRT1.1 in shoots and slightly decreased that in roots (Figure 1). Critical factors resulting in the discrepancy between the results reported by Liu et al (2015) and our study may be differences between the culture systems used. Furthermore, the variable NO 3 À concentrations and pH values in the growth medium may also account for the difference, as both affect NRT1.1 expression.…”

Section: Discussioncontrasting

confidence: 97%

“…Previously, Fe deficiency was found to downregulate the transcription level of NRT1.1 in the shoots and roots of plants grown in an agar system (Liu et al., 2015). In our hydroponic system, we showed that Fe deficiency markedly downregulated the level of NRT1.1‐GFP in both the shoots and roots of plants, but did not affect the gene expression of NRT1.1 in shoots and slightly decreased that in roots (Figure 1).…”

Section: Discussionmentioning

confidence: 98%

“…NRT1.1 was the first NO 3 − transporter identified in higher plants (Tsay et al., 1993), and it contributes to a majority of the plant NO 3 − uptake in NO 3 − ‐sufficient growth conditions (Huang et al., 1996; Liu et al., 1999; Liu & Tsay, 2003; Wang et al., 1998; Ye et al., 2019). A study showed that loss of NRT1.1 function in Arabidopsis improves plant tolerance to Fe deficiency (Liu et al., 2015), indicating a negative effect of NRT1.1 on Fe nutrition of plants. However, the exact role of NRT1.1 in plant Fe nutrition remains elusive.…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of shoot‐expressed NRT1.1 improves reutilization of apoplastic iron under iron‐deficient conditions

Zhou

Zhu

et al. 2022

The Plant Journal

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SUMMARY Iron deficiency is a major constraint for plant growth in calcareous soils. The interplay between NO3− and Fe nutrition affects plant performance under Fe‐deficient conditions. However, how NO3− negatively regulates Fe nutrition at the molecular level in plants remains elusive. Here, we showed that the key nitrate transporter NRT1.1 in Arabidopsis plants, especially in the shoots, was markedly downregulated at post‐translational levels by Fe deficiency. However, loss of NRT1.1 function alleviated Fe deficiency chlorosis, suggesting that downregulation of NRT1.1 by Fe deficiency favors plant tolerance to Fe deficiency. Further analysis showed that although disruption of NRT1.1 did not alter Fe levels in both the shoots and roots, it improved the reutilization of apoplastic Fe in shoots but not in roots. In addition, disruption of NRT1.1 prevented Fe deficiency‐induced apoplastic alkalization in shoots by inhibiting apoplastic H+ depletion via NO3− uptake. In vitro analysis showed that reduced pH facilitates release of cell wall‐bound Fe. Thus, foliar spray with an acidic buffer promoted the reutilization of Fe in the leaf apoplast to enhance plant tolerance to Fe deficiency, while the opposite was true for the foliar spray with a neutral buffer. Thus, downregulation of the shoot‐part function of NRT1.1 prevents apoplastic alkalization to ensure the reutilization of apoplastic Fe under Fe‐deficient conditions. Our findings may provide a basis for elucidating the link between N and Fe nutrition in plants and insight to scrutinize the relevance of shoot‐expressed NRT1.1 to the plant response to stress.

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Section: Discussioncontrasting

confidence: 97%

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Inhibition of shoot‐expressed NRT1.1 improves reutilization of apoplastic iron under iron‐deficient conditions

Zhou

Zhu

et al. 2022

The Plant Journal

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“…Further studies are needed to investigate the relevant downstream signal transduction pathways of this N-P integrator. Liu et al (2015) reported that the lack of NRT1.1 enhances plant tolerance to Fe deficiency stress; however, the expression of Fe acquisition related-genes FRO2, IRT1, and FIT was lower in the nrt1.1 mutants than in wild-type plants under Fe-deficient conditions, indicating that the FIT-dependent Fe deficiency signalling pathway was not involved in NRT1.1regulated Fe deficiency responses. Because nitrate functions as a nutrient and a signalling molecule (Krouk, 2017), it is conceivable that the reduced accumulation of internal nitrate in nrt1.1 mutants may impair the FIT-dependent Fe deficiency signalling pathway.…”

Section: P and Fe Deficiencymentioning

confidence: 97%

NRT1.1 Dual-Affinity Nitrate Transport/Signalling and its Roles in Plant Abiotic Stress Resistance

Fang

et al. 2021

Front. Plant Sci.

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NRT1.1 is the first nitrate transport protein cloned in plants and has both high- and low-affinity functions. It imports and senses nitrate, which is modulated by the phosphorylation on Thr101 (T101). Structural studies have revealed that the phosphorylation of T101 either induces dimer decoupling or increases structural flexibility within the membrane, thereby switching the NRT1.1 protein from a low- to high-affinity state. Further studies on the adaptive regulation of NRT1.1 in fluctuating nitrate conditions have shown that, at low nitrate concentrations, nitrate binding only at the high-affinity monomer initiates NRT1.1 dimer decoupling and priming of the T101 site for phosphorylation activated by CIPK23, which functions as a high-affinity nitrate transceptor. However, nitrate binding in both monomers retains the unmodified NRT1.1, maintaining the low-affinity mode. This NRT1.1-mediated nitrate signalling and transport may provide a key to improving the efficiency of plant nitrogen use. However, recent studies have revealed that NRT1.1 is extensively involved in plant tolerance of several adverse environmental conditions. In this context, we summarise the recent progress in the molecular mechanisms of NRT1.1 dual-affinity nitrate transport/signalling and focus on its expected and unexpected roles in plant abiotic stress resistance and their regulation processes.

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“…In addition to its function as NO 3 - transporter, NRT1.1 also plays important roles in vegetative and reproductive growth (Guo et al, 2001), stomatal opening (Guo et al, 2003), root architecture (Remans et al, 2006; Mounier et al, 2014), and transport of chloride and phytohormones (IAA/ABA/jasmonic acid/GAs) (Tsay et al, 1993; Guo et al, 2002; Krouk, 2016; Corratgé-Faillie and Lacombe, 2017). Moreover, it induces tolerance in plants to abiotic stresses, such as proton stress, salt stress, Cd 2+ stress, and iron deficiency (Mao et al, 2014; Liu et al, 2015; Abouelsaad et al, 2016; Fang et al, 2016). NRT1.1-mediated plant stress tolerance is closely associated with NO 3 - uptake, assimilation, and accumulation.…”

Section: Introductionmentioning

confidence: 99%

NRT1.1 Regulates Nitrate Allocation and Cadmium Tolerance in Arabidopsis

et al. 2019

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Abiotic stress induces nitrate (NO 3 - ) allocation to roots, which increases stress tolerance in plants. NRT1.1 is broadly involved in abiotic stress tolerance in plants, but the relationship between NRT1.1 and NO 3 - allocation under stress conditions is unclear. In this study, we found that Arabidopsis wild-type Col-0 was more cadmium (Cd 2+ )-tolerant than the nrt1.1 mutant at 20 μM CdCl 2 . Cd 2+ exposure repressed NRT1.5 but upregulated NRT1.8 in roots of Col-0 plants, resulting in increased NO 3 - allocation to roots and higher [NO 3 - ] root-to-shoot (R:S) ratios. Interestingly, NITRATE REGULATORY GENE2 ( NRG2 ) was upregulated by Cd 2+ stress in Col-0 but not in nrt1.1 . Under Cd 2+ stress, nrg2 and nrg2-3chl1-13 mutants exhibited similar phenotypes and NO 3 - allocation patterns as observed in the nrt1.1 mutant, but overexpression of NRG2 in Col-0 and nrt1.1 increased the [NO 3 - ] R:S ratio and restored Cd 2+ stress tolerance. Our results indicated that NRT1.1 and NRG2 regulated Cd 2+ stress-induced NO 3 - allocation to roots and that NRG2 functioned downstream of NRT1.1 . Cd 2+ uptake did not differ between Col-0 and nrt1.1 , but Cd 2+ allocation to roots was higher in Col-0 than in nrt1.1 . Stressed Col-0 plants increased Cd 2+ and NO 3 - allocation to root vacuoles, which reduced their cytosolic allocation and transport to the shoots. Our results suggest that NRT1.1 regulates NO 3 - allocation to roots by coordinating Cd 2+ accumulation in root vacuoles, which facilitates Cd 2+ detoxification.

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The nitrate transporter NRT1.1 is involved in iron deficiency responses in Arabidopsis

Cited by 13 publications

References 53 publications

Inhibition of shoot‐expressed NRT1.1 improves reutilization of apoplastic iron under iron‐deficient conditions

Inhibition of shoot‐expressed NRT1.1 improves reutilization of apoplastic iron under iron‐deficient conditions

NRT1.1 Dual-Affinity Nitrate Transport/Signalling and its Roles in Plant Abiotic Stress Resistance

NRT1.1 Regulates Nitrate Allocation and Cadmium Tolerance in Arabidopsis

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