2014
DOI: 10.1155/2014/840863
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Evaluation the Surface Antigen of theSalmonella typhimuriumATCC 14028 Ghosts Prepared by “SLRP”

Abstract: Recently, bacterial ghosts (BGs) were prepared using a protocol based on critical chemical concentrations. It has been given the name “sponge like” (SL) protocol and used in its reduced form “sponge like reduced protocol” (SLRP). While specific antibody for Salmonella is available on the market under the commercial names (of some kits) such as Febrile Antigen Kit (N.S. BIO-TEC), we used the described Kit to investigate the validity of the SLRP. In this study, using SLRP we succeeded to prepare STGs with corre… Show more

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Cited by 18 publications
(17 citation statements)
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“…The protocol describes a new method for bacterial evacuation from their cytoplasmic contents without deforming their 3D structure or their surface antigens [1,2]. The protocol was originally optimized by Plackett-Burman design [1,3].…”
Section: Dear Editormentioning
confidence: 99%
See 1 more Smart Citation
“…The protocol describes a new method for bacterial evacuation from their cytoplasmic contents without deforming their 3D structure or their surface antigens [1,2]. The protocol was originally optimized by Plackett-Burman design [1,3].…”
Section: Dear Editormentioning
confidence: 99%
“…The used chemical compounds are SDS, NaOH, H 2 O 2 , CaCO 3 and the NaCl and the Ethanol were used during the evacuation processes. By determining each of the MIC and MGC and running either the full Plackett-Burman twelve experimental design as in the original protocol or running the best two experiments which giving the best results as in the reduced protocol, that guarantee full evacuation with nearly no effect on the 3D or the surface antigens [4,2].…”
Section: Dear Editormentioning
confidence: 99%
“…BGs can be used in the diagnostic Kit as a reference antigen, where it should give positive reaction with serum containing the proper antibodies [34].…”
Section: Using Mgs (Figure 3)mentioning
confidence: 99%
“…Its main concept is using active chemical compounds that could introduce pores in the microbes and degrade the DNA at concentrations which did not change the surface antigens or the 3D structure [13]. This enables evacuating gram-negative and gram-positive bacteria, eukaryotes and viruses [4,[12][13][14][16][17][18][19][20][21][22][23][24][25][26][27].…”
Section: Introductionmentioning
confidence: 99%