Evaluation of xylose-fermenting yeasts for ethanol production from spent sulfite liquor

Björling, Torsten; Lindman, Björn

doi:10.1016/0141-0229(89)90099-9

Cited by 67 publications

(19 citation statements)

References 16 publications

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“…1989; Schneider 1989). They ferment xylose in acid prehydrolysates and spent sulphite liquor (SSL) at a pH of around 5·5 (Bjorling and Lindman 1989; Parekh et al . 1987).…”

Section: Discussionmentioning

confidence: 99%

“…At pH 5·0, the adapted culture could grow and produce ethanol, whereas the parent culture was inhibited (Table 3). Bjorling and Lindman (1989) reported complete inhibition of ethanol production by P. stipitis in a medium containing 3·9 g l −1 acetic acid. The pH‐dependent inhibition of growth and ethanol production has been described previously (Chu et al .…”

Section: Discussionmentioning

confidence: 99%

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Development of xylose-fermenting yeast Pichia stipitis for ethanol production through adaptation on hardwood hemicellulose acid prehydrolysate

Nigam

2001

J Appl Microbiol

118

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Aims: The objective of this study was to develop a mutant from Pichia stipitis NRRL Y-7124, tolerant of high concentrations of acetic acid and other inhibitory components present in acid hydrolysates, to improve ethanol yield and productivity. Methods and Results: The mutant was developed through adaptation in acid hydrolysate supplemented with nutrients and minerals at 30 0á5°C. When it was tested for its ability to ferment acid hydrolysate, it showed shorter fermentation time, better tolerance to acid and could ferment at lower pH. The ethanol yield (Y p/s ) and productivity (Q p ) were increased 1á6-and 2á1-fold, respectively. Conclusions: The development of a mutant and its tolerance to acetic acid present in hydrolysates is described. The selected mutant is capable of fermenting both hexoses and pentoses present in hydrolysate at lower pH in comparison with the parent strain. Signi®cance and Impact of the Study: The mutant could play a signi®cant role in reducing environmental pollution by using sugars present in pulp mill ef¯uent and, at the same time, could produce a marketable liquid fuel ethanol.

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“…1989; Schneider 1989). They ferment xylose in acid prehydrolysates and spent sulphite liquor (SSL) at a pH of around 5·5 (Bjorling and Lindman 1989; Parekh et al . 1987).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Development of xylose-fermenting yeast Pichia stipitis for ethanol production through adaptation on hardwood hemicellulose acid prehydrolysate

Nigam

2001

J Appl Microbiol

118

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“…1119 VoL 121-124, 2005 sustain their viability required for efficient bioconversion (7)(8)(9)(10)(11)(12)(13). In order to utilize these soluble sugars, the contaminated hydrolyzates must be cleaned and detoxified before they are subjected to bioconversion.…”

Section: Applied Biochemistry and Biotechnologymentioning

confidence: 99%

Pretreatment of Corn Stover by Soaking in Aqueous Ammonia

Kim

Lee

Twenty-Sixth Symposium on Biotechnology for Fuels and Chemicals

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Soaking in aqueous ammonia (SAA) was investigated as a pretreatment method for corn stover. In this method, the feedstock was soaked in aqueous ammonia over an extended period (10-60 d) at room temperature. It was done without agitation at atmospheric pressure. SAA treatment removed 55-74% of the lignin, but retained nearly 100% of the glucan and 85% of the xylan. The xylan remaining in the corn stover after SAA treatment was hydrolyzed along with the glucan by xylanase present in the Spezyme CP enzyme. In the simultaneous saccharification and fermentation (SSF) test of SAA-treated corn stover, using S. cerevisiae (DsA), an ethanol yield of 73% of theoretical maximum was obtained on the basis of the glucan content in the treated corn stover. The accumulation of xylose in the SSF appears to inhibit the cellulase activity on glucan hydrolysis, which limits the yield of ethanol. In the simultaneous saccharification and co-fermentation (SSCF) test, using recombinant E. coli (KOll), both the glucan and xylose were effectively utilized, resulting in on overall ethanol yield of 77% based on the glucan and xylan content of the substrate. When the SSCF process is used, the fact that the xylan fraction is retained during pretreatment is a desirable feature since the overall bioconversion can be carried out in a single step without separate recovery of xylose from the pretreatment liquid.

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“…Lignocellulose is renewable, and due to its abundance in nature in some areas of the world, this carbohydrate is potentially attractive for production of value-added products. Production of ethanol to be used as a liquid fuel is a well-described example of this (5,7,37,51,76,100). This is an attractive application from an environmental point of view, since no net carbon dioxide is released from combustion of this carbon source (38,81).…”

Section: Xylose Utilizationmentioning

confidence: 99%

Metabolic Engineering of Saccharomyces cerevisiae

Østergaard

Olsson

Nielsen

2000

Microbiol Mol Biol Rev

405

213

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SUMMARY Comprehensive knowledge regarding Saccharomyces cerevisiae has accumulated over time, and today S. cerevisiae serves as a widley used biotechnological production organism as well as a eukaryotic model system. The high transformation efficiency, in addition to the availability of the complete yeast genome sequence, has facilitated genetic manipulation of this microorganism, and new approaches are constantly being taken to metabolicially engineer this organism in order to suit specific needs. In this paper, strategies and concepts for metabolic engineering are discussed and several examples based upon selected studies involving S. cerevisiae are reviewed. The many different studies of metabolic engineering using this organism illustrate all the categories of this multidisciplinary field: extension of substrate range, improvements of producitivity and yield, elimination of byproduct formation, improvement of process performance, improvements of cellular properties, and extension of product range including heterologous protein production.

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Evaluation of xylose-fermenting yeasts for ethanol production from spent sulfite liquor

Cited by 67 publications

References 16 publications

Development of xylose-fermenting yeast Pichia stipitis for ethanol production through adaptation on hardwood hemicellulose acid prehydrolysate

Development of xylose-fermenting yeast Pichia stipitis for ethanol production through adaptation on hardwood hemicellulose acid prehydrolysate

Pretreatment of Corn Stover by Soaking in Aqueous Ammonia

Metabolic Engineering of Saccharomyces cerevisiae

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