Development of xylose-fermenting yeast Pichia stipitis for ethanol production through adaptation on hardwood hemicellulose acid prehydrolysate

Nigam, J. N.

doi:10.1046/j.1365-2672.2001.01234.x

Cited by 118 publications

(88 citation statements)

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“…Biofuels 9, 167. doi:10.1186/s13068-016-0570-6 vanillin, syringaldehyde, and hydroxybenzoic acid have also been developed in ethanologenic yeast by approaching adaptation strategy (Landaeta et al, 2013;Slininger et al, 2015). In another study, adaptation of S. stipitis was performed in acid hydrolyzate and evolved strain showed enhanced tolerance with improved fermentation of hydrolyzate in shorter duration (Nigam, 2001). Similarly, the evolutionary engineering of S. stipitis has been done in hardwood spent sulfite liquor and various evolved strains with improved tolerance to inhibitor present in liquor have been obtained (Pereira et al, 2015).…”

Section: −1mentioning

confidence: 99%

Evolutionary Adaptation of Kluyveromyces marxianus NIRE-K3 for Enhanced Xylose Utilization

et al. 2017

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The evolutionary adaptation was approached on the thermotolerant yeast Kluyveromyces marxianus NIRE-K3 at 45°C on xylose as a sole source of carbon for enhancement of xylose uptake. After 60 cycles, evolved strain K. marxianus NIRE-K3.1 showed comparatively 3.75-and 3.0-fold higher specific growth and xylose uptake rates, respectively, than that of native strain. Moreover, the short lag phase was also observed on adapted strain. During batch fermentation with xylose concentration of 30 g l −1, K. marxianus NIRE-K3.1 could utilize about 96% of xylose in 72 h and produced 4.67 and 15.7 g l −1 of ethanol and xylitol, respectively, which were 9.72-and 4.63-fold higher than that of native strain. Similarly, specific sugar consumption rate, xylitol, and ethanol yields were 5.07-, 1.15-, and 2.44-fold higher as compared to the native strain, respectively. The results obtained after evolutionary adaptation of K. marxianus NIRE-K3 show the significant improvement in the xylose utilization, ethanol and xylitol yields, and productivities. By understanding the results obtained, the significance of evolutionary adaptation has been rationalized, since the adapted culture could be more stable and could enhance the productivity.

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Section: −1mentioning

confidence: 99%

Evolutionary Adaptation of Kluyveromyces marxianus NIRE-K3 for Enhanced Xylose Utilization

et al. 2017

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“…When it was tested for its ability to ferment acid hydrolyzate, it showed shorter fermentation time, better tolerance to acid and could ferment at lower pH. The ethanol yield and productivity were increased 1.3 and 2.1 fold, respectively [16]. However, more researchers investigated detoxification methods to detoxify the hydrolyzate to improve fermentation efficiency.…”

Section: Introductionmentioning

confidence: 99%

Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate

Zhu¹,

Yong²,

Xu³

et al. 2009

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Yeast Pichia stipitis CBS 5776 was developed through adaptation fermentation step by step in steam exploded corn stover prehydrolyzate because high concentration of weak acids and other inhibitors present in the prehydrolyzate could degrade the fermentability. However, the adaptability of Pichia stipitis CBS 5776 in the prehydrolyzate was so limited that steam stripping and overliming were applied to remove these inhibitors from it. Corn stover was steam exploded; the filtrate of steam exploded corn stover was hydrolyzed with dilute sulfuric acid, and then the acid hydrolyzate was detoxified and fermented by Pichia stipitis CBS 5776. Steam stripping could remove volatile compounds from the acid hydrolyzate and the filtrate. At a steam stripping time of 120min, 81% acetic acid and 59% formic acid were removed from the acid hydrolyzate, 77% acetic acid and 45% formic acid were removed from the filtrate, while furfural was stripped off completely from the acid hydrolyzate and the filtrate. Overliming could reduce the contents of furfural and phenolics present in the acid hydrolyzate, however, sugars, especially pentoses, were also removed partially. It was necessary to detoxify the acid hydrolyzate in order to ferment the sugars to ethanol. Acid hydrolyzate detoxified with a combination of steam stripping for 120 min and overliming at pH11 and 60℃ for 90 min, its fermentability was significantly improved. Xylose was consumed nearly completely in 24h with an ethanol yield of 15.92g/l, 80.34% of theoretical.

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“…It has been reported that fermentations of steam exploded wheat straw by S. stipitis were completely inhibited by a synergistic effect due to the presence of 1.5 g L -1 of acetic acid, 0.15 g L -1 of furfural and 0.05 g L -1 of HMF (Bellido et al, 2011), the same acetic acid concentration found in this work after detoxification. However, the development of a P. stipitis more tolerant to acetic acid and other inhibitory components present in acid hydrolysates is possible through a simple adaptation of cells (Nigam, 2001).…”

Section: Methodsmentioning

confidence: 99%

Valorization of sunflower meal through the production of ethanol from the hemicellulosic fraction

Tavares

Sene

Christ

2016

Rev. bras. eng. agríc. ambient.

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A B S T R A C TSunflower is among the major oil seeds crop grown in the world and the by-products generated during the seeds processing represent an attractive source of lignocellulosic biomass for bioprocesses. The conversion of lignocellulosic fibers into fermentable sugars has been considered as a promising alternative to increase the demand for ethanol. The present study aimed to establish the fermentation conditions for ethanol production by Scheffersomyces stipitis ATCC 58376 in sunflower meal hemicellulosic hydrolysate, through a 2 3 CCRD (Central Composite Rotational Design) factorial design. Under the selected conditions (pH 5.25, 29 ºC and 198 rpm) the final ethanol concentration was 13.92 g L -1 and the ethanol yield was 0.49 g g -1 .Valorização do farelo de girassol através da produção de etanol a partir da fração hemicelulósica R E S U M O O girassol está entre as principais oleaginosas cultivadas no mundo e os subprodutos gerados durante o processamento das sementes representam uma fonte atraente de biomassa lignocelulósica para bioprocessos. A conversão de fibras lignocelulósicas em açúcares fermentáveis tem sido considerada uma alternativa promissora para aumentar a demanda de etanol. O presente estudo teve, como objetivo, estabelecer as condições de fermentação para a produção de etanol por Scheffersomyces stipitis ATCC 58376 em hidrolisado hemicelulósico de farelo de girassol através de um planejamento fatorial DCCR 2 3 . Sob as condições selecionadas (pH 5,25, 29 ºC e 198 rpm) a concentração final de etanol foi 13,92 g L -1 e o rendimento 0,49 g g -1 .

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Development of xylose-fermenting yeast Pichia stipitis for ethanol production through adaptation on hardwood hemicellulose acid prehydrolysate

Cited by 118 publications

References 20 publications

Evolutionary Adaptation of Kluyveromyces marxianus NIRE-K3 for Enhanced Xylose Utilization

Evolutionary Adaptation of Kluyveromyces marxianus NIRE-K3 for Enhanced Xylose Utilization

Adaptation fermentation of Pichia stipitis and combination detoxification on steam exploded lignocellulosic prehydrolyzate

Valorization of sunflower meal through the production of ethanol from the hemicellulosic fraction

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