Evaluation of Wound Healing Potential of Novel Hydrogel Based on Ocimum basilicum and Trifolium pratense Extracts

Abstract

Plants are an inexhaustible source of compounds with different medicinal properties, suitable as alternative options for the prevention and treatment of various pathologies. They are safe, effective and economical. In this paper, a combined extract made of Ocimum basilicum and Trifolium pratense extracts (EOT) was used for the first time to demonstrate its healing effect on dermal pathologies. To evaluate the wound healing effect of EOT, a novel gel formulation was prepared and subsequently tested in vitro (using the scratch test assay) and in vivo (on an animal model). The in vitro tests demonstrated the complete recovery of the dermal fibroblast monolayer when treated with EOT in a concentration of 50 µg/mL. In vivo results using a hydrogel formulation based on EOT demonstrated improved wound contraction time and complete healing after 13 days of treatment. Moreover, a clinical case of Psoriasis vulgaris was presented, in which one week of treatment led to the significant improvement of the patient’s health. In conclusion, the topical use of the novel gel formulation containing EOT is a successful therapeutic alternative in the treatment of dermal diseases.

Formulation and Characterization of Hydrophilic Ointment Bases with Carvacrol and Magnolol for Periodontal Application

“…To formulate preparations with good tolerability to the oral mucosa, we chose excipients suitable for the application site, free of irritating action, and chemically and pharmacologically inert. For this purpose, we used carbopolies as excipients, with or without adjuvants [38,39].…”

Evaluation of In Vitro Wound-Healing Potential, Antioxidant Capacity, and Antimicrobial Activity of Stellaria media (L.) Vill

“…Wound cell density is a parameter that must be considered to determine the most effective treatment applied [20]. Figure 5 shows the evolution of cell density inside the wound depending both on the treatment applied and time (T0 to 48 h) compared to the initial density.…”

“…The density of NHDF is a defining parameter in the wound-healing process, being the one that highlights the most effective treatment [18][19][20]. Figure 5 shows the cell density inside the wound depending on both the treatment applied and the time.…”

The Interdependence Between Diet, Microbiome, And Human Body Health - A Systemic Review

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