Evaluation of white rot fungi pretreatment of mushroom residues for volatile fatty acid production by anaerobic fermentation: Feedstock applicability and fungal function

Abstract

White rot fungi using P. sajor-caju and T. versicolor was examined to pretreat raw champost (lignin-rich) and oyster champost (cellulose-rich) for enhancement of fermentative volatile fatty acid (VFA) production. Results showed that the efficiency of pretreatment and VFA production was influenced by the fungal strains and substrates. P. sajor-caju pretreatment showed preferential lignin degradation on raw champost and obtained the maximum VFA yield (203 ± 9 mg COD/g VS added), which increased by 60% and 74% compared to that of control and unpretreated champost, respectively. For cellulose-rich oyster champost, however, fungal pretreatment decreased VFA yield compared to unpretreated champost. Further mechanisms analysis demonstrated the two strains grow and secreted ligninolytic enzymes, which substantially influenced the characteristics of two champosts such as cellulose/lignin ratio and morphology in different extents. P. sajor-caju was highly efficient to lignin-rich champost on selectively degrading lignin and further enhancing digestibility such as VFA production.

Microorganisms and Enzymes Used in the Biological Pretreatment of the Substrate to Enhance Biogas Production: A Review

“…Fang et al [110] pretreated raw and oyster champost with fungal strains Trametes versicolor and Pleurotus sajor-caju for improvement of fermentative volatile fatty acid generation. Pleurotus sajor-caju was the most effective to raw champost on selectively degrading lignin and improved volatile fatty acid production.…”

Biological pretreatment of wheat straw: Effect of fungal culturing on enzymatic hydrolysis of carbohydrate polymers

“…Studied fungal species exhibited various effectiveness in increasing sacchari cation which was mostly expected due to different physiological response of each fungus, in uenced by different physical and chemical factors during the cultivation period 35 . Thus, fungal culturing cannot always increase hydrolysis yield, and the positive effect depends on fungal species, the range of cultivation conditions and the substrate properties 36 . Besides our study, the positive effect of pretreatment with white-rot fungi has also been observed by other authors 17,18,36 .…”

“…Thus, fungal culturing cannot always increase hydrolysis yield, and the positive effect depends on fungal species, the range of cultivation conditions and the substrate properties 36 . Besides our study, the positive effect of pretreatment with white-rot fungi has also been observed by other authors 17,18,36 . The increase of hydrolysis yields is directly correlated to a better accessibility to polysaccharides after fungal deligni cation.…”

Estimation of glucosamine in biomass of Trichoderma reesei cultivated on lignocellulosic substrates

“…Many researchers have studied the SSF of lignocellulosic materials using Trichoderma reesei as a highly efficient producer of lignocellulose‐degrading enzymes [13–15]. To overcome the problem of mycelial (fungal biomass) separation from the solid substrate [16,17], most researchers investigated fungal growth by indirect biomass analyses, such as flow cytometry [18], measurement of accumulated CO 2 [19], and the determination of fungal components that are proportional to growth, such as ergosterol [20] and protein [21]. One of the methods commonly used for indirect biomass assessment is the measurement of glucosamine [22–26], which is a monomer of chitin existing in fungal cell walls.…”