Estimation of glucosamine in biomass of <i>Trichoderma reesei</i> cultivated on lignocellulosic substrates

Chysirichote, Teerin; Mapisansup, Waraporn; Aroonsong, Soysrung

doi:10.1002/jobm.202000609

Cited by 2 publications

(3 citation statements)

References 41 publications

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“…The obtained glucosamine content in the dry fermented substrate (Glu, mg g –1 ) was used to calculate the biomass dry weight of T. reesei by using the equation studied by Chysirichote et al The analyses were performed in triplicate.…”

Section: Methodsmentioning

confidence: 99%

“…First, 10 mg of the sample was immersed in a 60% sulfuric acid solution at room temperature for 24 h. The amount of glucosamine was analyzed using the colorimetric method. 36 The obtained glucosamine content in the dry fermented substrate (Glu, mg g −1 ) was used to calculate the biomass dry weight of T. reesei by using the equation studied by Chysirichote et al 37 The analyses were performed in triplicate.…”

Section: Preparation Of the Solid Substrate For Fermentationmentioning

confidence: 99%

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High Production of Cellulase and Xylanase in Solid-State Fermentation by Trichoderma reesei Using Spent Copra and Wheat Bran in Rotary Bioreactor

Chysirichote

Phaiboonsilpa

Laosiripojana

2023

Ind. Eng. Chem. Res.

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The enzyme production for lignocellulose saccharification by solid-state fermentation (SSF) of a food manufacturing byproduct was successfully carried out in a 30 L rotary bioreactor. Defatted spent copra (SC) supplemented with wheat bran (WB) was used as a substrate for the SSF of Trichoderma reesei and aerated at various rates. Regression analysis showed that the carbohydrate/protein (C/P) ratio of the substrate and the supplied aeration rate were the important factors for producing the enzyme cocktail, including cellulases (FPase, CMCase, and cellobiase) and xylanase. The substrate containing SC:WB of 3:2 (or the C/P ratio of 5.4) and the aeration of 1.0 L kg −1 substrate min −1 were found to enhance the production of the enzymes up to 5.68, 8.66, 29.2, and 34.44 U g −1 of dry substrate for FPase, CMCase, cellobiase, and xylanase activities, respectively. This discovery provided a promising environment for other substrates to produce multi-enzymes for lignocellulosic saccharification. Additionally, mathematical models were generated to predict the saccharifying degree of the produced enzyme for lignocellulose saccharification.

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Section: Methodsmentioning

confidence: 99%

Section: Preparation Of the Solid Substrate For Fermentationmentioning

confidence: 99%

High Production of Cellulase and Xylanase in Solid-State Fermentation by Trichoderma reesei Using Spent Copra and Wheat Bran in Rotary Bioreactor

Chysirichote

Phaiboonsilpa

Laosiripojana

2023

Ind. Eng. Chem. Res.

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“…Approximately, 10 mg of dry biomass (known actual weight) was determined for its glucosamine content using a colorimetric method (Chysirichote et al, 2021). In short, the sample was hydrolysed by soaking it for 24 h at room temperature in 1 mL of 60% (v/v) sulphuric acid.…”

Section: Growth Evaluationmentioning

confidence: 99%

Correlation of oxygen consumption with growth and red pigment production of Monascus purpureus in solid state fermentation of rice

Chysirichote,

Sakoolkaew,

Klinthong

2024

IFRJ

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Since aeration has a crucial role in mass and heat transfer during solid-state fermentation (SSF), the aim of the present work was to find out how it related to biomass and red pigment production of Monascus purpureus TISTR3615 during SSF. The fungal consumption of oxygen, one of the air's constituents important for fungal metabolism, was used to explain the relationship between the aeration rate, the fungal growth, and the red pigment production during the SSF of rice. Findings showed that at increased aeration rates, the fungus absorbed more oxygen, which boosted fungal growth but decreased red pigment production. Conversely, red pigment production increased when the fungus' oxygen consumption decreased. Regression equations with an acceptable R2 were used to explain the relationship between M. purpureus' red pigment production and its oxygen consumption and aeration rate. The ideal oxygen concentration for increasing M. purpureus' capacity to produce red pigment was 0.0685 mmol/day/g of dry biomass, which produced red pigment at around 2,897 AU500nm/g of dry biomass. However, the optimal oxygen consumption of the fungus for overall red pigment production (including growth impact) was 1.390 mmol/day/g of dry biomass, which produced red pigment at around 2,787 AU500nm/g of dry fermented rice. The results of the present work could potentially be utilised in developing M. purpureus' red pigment production approach.

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Estimation of glucosamine in biomass of Trichoderma reesei cultivated on lignocellulosic substrates

Cited by 2 publications

References 41 publications

High Production of Cellulase and Xylanase in Solid-State Fermentation by Trichoderma reesei Using Spent Copra and Wheat Bran in Rotary Bioreactor

High Production of Cellulase and Xylanase in Solid-State Fermentation by Trichoderma reesei Using Spent Copra and Wheat Bran in Rotary Bioreactor

Correlation of oxygen consumption with growth and red pigment production of Monascus purpureus in solid state fermentation of rice

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