Evaluation of Wheat Germplasm for Resistance to Leaf Rust (Puccinia triticina) and Identification of the Sources of Lr Resistance Genes Using Molecular Markers

Кохметова, Алма; Rsaliyev, Shynbolat; Atishova, M. N.; Kumarbayeva, Madina; Malysheva, Angelina; Keishilov, Zhenis; Zhanuzak, D.K.; Bolatbekova, Ardak

doi:10.3390/plants10071484

Cited by 12 publications

(3 citation statements)

References 49 publications

(70 reference statements)

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“…In addition, many researchers have found that leaf rust antigens are relatively scarce in the main wheat cultivars, while leaf rust races show continuous variation, resulting in the gradual loss of resistance in many resistant cultivars (Kokhmetova et al 2021). Therefore, the identification of novel resistance genes will be useful for wheat leaf rust resistance breeding and analyzing the mechanisms underlying disease resistance.…”

Section: Discussionmentioning

confidence: 99%

Generation and identification of a wheat–Agropyron cristatum (L.) Gaertn. 3P chromosome addition line and substitution line

Kuang

et al. 2023

Euphytica

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Agropyron cristatum (L.) Gaertn. (2n=4x=28, PPPP), which is a wild relative of common wheat and has a P genome, has many excellent characteristics (for example, it is disease resistant, is stress resistant and is high yielding) and has important utilization value for the genetic improvement of wheat.In this study, the wheat-A. cristatum 3P addition line I-27 and 3P(3B) substitution line I-15 were identified for the first time via in situ hybridization (ISH) and specific molecular marker (EST-STS) from wheat-A. cristatum-derived lines, which laid an representing important material for the utilization of excellent genes from A. cristatum chromosome 3P for wheat genetic improvement. In terms of agronomic traits, that the tiller number and spike length of 3P addition line I-27 were significantly higher than those of Fukuhokomugi. The spike length and spikelet number per spike of 3P substitution line I-15 were significantly greater than those of Fukuho. Additionally, wheat-A. cristatum 3P substitution line I-15 was resistant to wheat leaf rust. The generation of the 3P addition line I-27 and 3P substitution line I-15 was essential for the transfer of the excellent genes of the A. cristatum 3P chromosome into common wheat and resulted in the wheat-A. cristatum addition line containing a complete set of genetic research materials, laying a foundation for comparative studies of the P genome and subsequent fine mapping.

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Section: Discussionmentioning

confidence: 99%

Generation and identification of a wheat–Agropyron cristatum (L.) Gaertn. 3P chromosome addition line and substitution line

Kuang

et al. 2023

Euphytica

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“…tritici [8], Puccinia triticina Erikss. [9], Tilletia caries [10]. In recent years there has been increasing distribution and harmfulness of P. tritici-repentis in Kazakhstan [11][12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Identification of wheat samples for resistance to toxins of Pyrenophora tritici-repentis (Ptr)

Kumarbayeva

Kovalenko²,

Kremneva³

et al. 2022

ijbch

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Pyrenophora tritici-repentis (Ptr) is a causal agent of tan spot in wheat in Kazakhstan, as it has been around the world. The pathogen produces host-specific toxins which interact with the wheat host sensitivity loci. The aim of this study was 1) to identify whether selected Kazakhstani isolates of P. triticirepentis possessed the Ptr toxin genes ToxA and/or ToxB and 2) to identify the wheat varieties resistant to HST ToxA and ToxB. As a result of the analysis of the frequency of occurrence of PTR races, it was found that races 7 (25%) and 8 (41.6%) dominate in isolates from southern Kazakhstan, and race 4 (62.5%) prevails in northern Kazakhstan. Twenty single spore isolates collected from wheat-growing areas of the South and North of Kazakhstan representing the P. tritici-repentis population were characterized for the presence of the Ptr ToxA and Ptr ToxB genes, using two gene specific primers. Eight (40%) Kazakhstani P. tritici-repentis isolates were positive for the ToxA gene, and two isolates (10%) were positive for the ToxB gene. ToxB gene was not previously found in our country, but the results of this study show the appearance of this toxin in south Kazakhstan. Eleven (64.7%) wheat varieties resistant to HST ToxA were identified using molecular markers linked to the tsn1 gene, insensitive to Ptr ToxA. The identified genotypes are recommended for use in breeding for wheat resistance to tan spot.

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“…The most effective method of plant protection is to develop resistant varieties. At present, more than 80 genes for leaf rust resistance have been identified in the genome of wheat, and its relatives [3], and molecular markers are commonly used to identify them [4]. Leaf rust resistance genes were designated as Lr in the catalogue of gene symbols [3].…”

Section: Introductionmentioning

confidence: 99%

Screening of the Bread Wheat Varieties for the Leaf Rust Resistance Gene Lr34/Yr18/Sr57/Pm38/Bdv1

Radchenko¹,

Sandetska²,

Morgun³

et al. 2022

TOASJ

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Background: The allelic composition of the gene Lr34/Yr18/Sr57/Pm38/Bdv1, which is associated with resistance to leaf rust in varieties of bread wheat (Triticum aestivum L.), has been investigated. Methods: Three DNA markers were used to determine the allelic state of the gene Lr34/Yr18/Sr57/Pm38/Bdv1: the co-dominant molecular genetic markers cssfr5 and csLV34 and the microsatellite marker Xgwm295. Results: Among 32 cultivars evaluated for resistance to leaf rust, 4 were highly resistant, 26 were resistant and 2 were moderately susceptible. Using the co-dominant marker cssfr5 based on the detection of the polymorphic state of one of the exons of the gene Lr34/Yr18/Sr57/Pm38/Bdv1, the Lr34(+) allele, which confers resistance to leaf rust, was found in 25% of the studied varieties. The coincidence between the results obtained with the markers cssfr5 and csLV34 was 84.5%. Conclusion: The data of the conducted molecular genetic analysis were supplemented by observations of the resistance of the studied varieties to leaf rust in the field. The obtained data can be used in breeding programs to develop new varieties and breeding lines with leaf rust resistance.

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Evaluation of Wheat Germplasm for Resistance to Leaf Rust (Puccinia triticina) and Identification of the Sources of Lr Resistance Genes Using Molecular Markers

Cited by 12 publications

References 49 publications

Generation and identification of a wheat–Agropyron cristatum (L.) Gaertn. 3P chromosome addition line and substitution line

Generation and identification of a wheat–Agropyron cristatum (L.) Gaertn. 3P chromosome addition line and substitution line

Identification of wheat samples for resistance to toxins of Pyrenophora tritici-repentis (Ptr)

Screening of the Bread Wheat Varieties for the Leaf Rust Resistance Gene Lr34/Yr18/Sr57/Pm38/Bdv1

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