Evaluation of VITEK 2 for analysis of Enterobacteriaceae using the Advanced Expert System (AES) versus interpretive susceptibility guidelines used at Dynacare Kasper Medical Laboratories, Edmonton, Alberta

Blondel-Hill, Edith; Hetchler, C.; Andrews, Dawn; Lapointe, Luc

doi:10.1046/j.1469-0691.2003.00697.x

Cited by 7 publications

(4 citation statements)

References 36 publications

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“…Livermore et al (29) demonstrated the capacity of VITEK 2 to detect and interpret resistance mechanisms with a high level of accuracy and standardization. According to Blondel-Hill et al (30), the AES system is both adaptable and applicable in clinical microbiology laboratories to enhance the accuracy of susceptibility results and the clinical relevance of therapeutic recommendations.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Klebsiella isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and determination of antimicrobial resistance with VITEK 2 advanced expert system (AES)

Karagöz¹,

Acar²,

Körkoca³

2015

Turk J Med Sci

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Background/aim: The purpose of the study was to evaluate the performance of the VITEK mass spectrometry (MS) (bioMérieux, France) system for the identification of Klebsiella spp. isolated from different sources. Moreover, while assessing the ability of the VITEK 2 automated expert system (AES) to recognize antimicrobial resistance patterns, the researchers have extended the study to compare VITEK 2 with the routine antimicrobial susceptibility testing method. Materials and methods:This study tested 51 Klebsiella spp. isolates that were isolated from environmental examples and clinical examples. Results of conventional methods and the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) MS were compared. Then, any differing results were compared against a reference 16S rRNA gene sequence, and when indicated, a recA sequencing analysis was done.Results: VITEK MS correctly identified 100% of the Klebsiella spp. isolates. There were two K. oxytoca isolates incorrectly identified to the species level with conventional methods according to the 16S rRNA gene sequencing analysis. In addition, a VITEK 2 AST-N261 card was used for the detection of extended spectrum beta-lactamases (ESBL). Using the VITEK 2 AES, ESBL positivity was found at the rate of 16.3% whereas this rate was 4.08% using the disk diffusion method. Conclusion:MALDI-TOF MS is a rapid and accurate method for the identification of Klebsiella spp. Moreover, the bioMérieux AES provides a useful laboratory tool for the interpretation of susceptibility results.

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Section: Discussionmentioning

confidence: 99%

Characterization of Klebsiella isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and determination of antimicrobial resistance with VITEK 2 advanced expert system (AES)

Karagöz¹,

Acar²,

Körkoca³

2015

Turk J Med Sci

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“…The double-disc synergy test showed the highest specificity and positive predictive value among all test meth-VOL. 24,2011 EXPERT SYSTEMS IN CLINICAL MICROBIOLOGY 543 on May 9, 2018 by guest http://cmr.asm.org/ ods, i.e., 97% and 98%, respectively. Considering the rather low specificity observed by that study, those authors recommended the use of a manual test for confirmation once an organism is reported to be positive for ESBL production by any of the semiautomated systems.…”

Section: Detection Of Extended-spectrum ␤-Lactamases In Gramnegative mentioning

confidence: 99%

“…Recognition is there-VOL. 24,2011 EXPERT SYSTEMS IN CLINICAL MICROBIOLOGY 547 on May 9, 2018 by guest http://cmr.asm.org/ fore complicated by the highly efficient transmission of the MBL structural gene to strains in which the carbapenem-resistant phenotypes differ and in which a variety of other antibiotic resistance mechanisms may occur, including ESBLs. Espedido et al (78) showed that susceptibility profiles generated by diagnostic algorithms in commonly used automated bacterial identification systems such as the Phoenix NMIC/ID-101 panel and Vitek Legacy GNS-424, Vitek 2 AST-N044, and Vitek 2 AST-N019 Gram-negative cards failed to reliably identify a metallo-␤-lactamase in strains of the Enterobacteriaceae.…”

Section: Carbapenem Resistancementioning

confidence: 99%

Expert Systems in Clinical Microbiology

Winstanley

Courvalin

2011

Clin Microbiol Rev

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SUMMARY This review aims to discuss expert systems in general and how they may be used in medicine as a whole and clinical microbiology in particular (with the aid of interpretive reading). It considers rule-based systems, pattern-based systems, and data mining and introduces neural nets. A variety of noncommercial systems is described, and the central role played by the EUCAST is stressed. The need for expert rules in the environment of reset EUCAST breakpoints is also questioned. Commercial automated systems with on-board expert systems are considered, with emphasis being placed on the “big three”: Vitek 2, BD Phoenix, and MicroScan. By necessity and in places, the review becomes a general review of automated system performances for the detection of specific resistance mechanisms rather than focusing solely on expert systems. Published performance evaluations of each system are drawn together and commented on critically.

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“…Susceptibility/resistance is determined based on growth or inhibition of growth in the presence of antimicrobials at a limited range of concentrations straddling the breakpoint. Numerous evaluations of the Vitek system have been published (Blondel‐Hill et al, 2003; Bosman et al, 2014; Rennie et al, 2008). Fritsche et al (2011) reported on identification of bacterial pathogens from veterinary specimens.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic clinical microbiology

Blondeau

Rankin

2021

Vet Pharm & Therapeutics

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Technological advancements have changed the way clinical microbiology laboratories are detecting and identifying bacterial, viral, parasitic, and yeast/fungal pathogens. Such advancements have improved sensitivity and specificity and reduce turnaround time to reporting of clinically important results. This article discusses and reviews some traditional methodologies along with some of the technological innovations introduced into diagnostic microbiology laboratories. Some insight to what might be available in the coming years is also discussed.

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Evaluation of VITEK 2 for analysis of Enterobacteriaceae using the Advanced Expert System (AES) versus interpretive susceptibility guidelines used at Dynacare Kasper Medical Laboratories, Edmonton, Alberta

Abstract: This study demonstrated the benefits and limitations of the bioMérieux AES. Automated knowledge-based systems provide a useful laboratory tool for the interpretation of susceptibility results.

Cited by 7 publications

References 36 publications

Characterization of Klebsiella isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and determination of antimicrobial resistance with VITEK 2 advanced expert system (AES)

Characterization of Klebsiella isolates by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and determination of antimicrobial resistance with VITEK 2 advanced expert system (AES)

Expert Systems in Clinical Microbiology

Diagnostic clinical microbiology

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