Evaluation of virulence of Fusarium solani isolates on pea

Abstract

The virulence of 166 <I>Fusarium solani</I> isolates collected in the Czech Republic from infected pea plants was evaluated. Based on the ability to produce phytotoxic naphtarazin (DHF – dihydrofusarubin), only two isolates from this collection (designated FS VG and FS We) corresponded with the characteristics of f.sp. <I> pisi</I>. Suitability and effectiveness of two methods of inoculation based on soaking of seed in the inoculum were evaluated. The possibility to use mixtures of inocula of different species (<I>F. solani f.sp. pisi</I> + <I>F. oxysporum</I> f.sp. <I> pisi</I> races 1 and 2) and of mixtures of two isolates with different virulences was explored. Employing an efficient testing method and the most virulent isolate FS VG, 184 semileafless types of field peas and garden peas were screened to find accessions with a higher level of resistance to <I>F. solani</I> f.sp. <I> pisi</I>.

QTL Analysis of Fusarium Root Rot Resistance in an Andean × Middle American Common Bean RIL Population

“…sudden death syndrome, F. brasiliense, F. cuneirostrum , F. phaseoli , and F. virguliforme were found to have intraspecific variation in pathogenicity on soybean (Aoki et al, 2005). Ondrej et al (2008) observed that different isolates of F. oxysporum and F. solani had variations in virulence on pea ( Pisum sativum L.). We observed that when an isolate had mild virulence, such as the F. solani isolate tested in this study, the increase of inoculum amount did not have an effect on the severity of disease symptoms, but when the isolate had severe virulence, the amount of inoculum did affect the disease symptom expression, most notably on susceptible genotypes.…”

Distribution of organic metabolites after Fusarium wilt incidence in tomato (Solanum lycopersicum L.)

“…The isolate was tested for pathogenicity in a preliminary study of several contrasting genotypes at both the seedling and adult stages of development following the protocol of Ondrej et al, (2008). The isolate demonstrated strong virulence in the preliminary test and was therefore deemed suitable for the current experiment.…”

Alarming occurrence of Fusarium wilt disease in pea (Pisum sativum L.) cultivations of Jabalpur district in Central India revealed by an array of pathogenicity tests

“…The plates were incubated at 25 o C for seven days and observed after every 24 hours. Infection of F. oxysporum on pea seeds of the selected varieties was studied and evaluated on a 30 point scale (modification of method by Ondrej et al, 2008;Gunawardena, 2005). If a germinated seed showed coat infection as well as radical tip infection then two points or if either was true then one point was given.…”