Evaluation of vasogenic edema in experimental brain tumors by cathodoluminescence and fluorescence microscopy

Hürter, T; Bröcker, W.; Ka, Hossmann

doi:10.1007/bf00517138

Cited by 8 publications

(3 citation statements)

References 12 publications

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“…With AA, the reason for the variable density was not apparent by histological examination. It is possible that the low density is related to increased intra and extracellufar water content of the tumor, a parameter not measured in this study (54,55).…”

Section: Comparison Of Ct Finding With Human Brain Tumorsmentioning

confidence: 57%

Correlation of neuropathologic findings, computerized tomographic and high-resolution ultrasound scans of canine avian sarcoma virus-induced brain tumors

et al. 1987

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The location, size, geometry and neuropathological findings of anaplastic astrocytomas (AA), gliosarcomas and sarcomas induced by the avian sarcoma virus (ASV) in dogs were compared with images generated using computerized tomography (CT) and real time high-resolution ultrasound (HRUS). Seven AA showed a wide range of findings on CT. Pre-contrast CT scans showed that the tumors could be hyper, hypo, or isodense. Three of seven AA had no contrast enhancement; two of these tumors were also isodense which resulted in a false-negative CT exam. Partial enhancement was seen in one tumor. This resulted in a sensitivity of detection of 72%. Real time HRUS was able to define tumor location, size and geometry of the AA missed or incompletely imaged by CT. All tumors were hyperechoic. Inhomogeneity of the echo pattern was due to hemorrhage, cyst formation, and necrosis within the tumors. Such secondary tumor characteristics were more accurately defined by HRUS compared to CT. Vasogenic edema in the brain surrounding tumors was of low density on CT and hypoechoic or indistinguishable from normal brain on US. Similar findings were seen in six gliosarcomas, two of which were not visualized by either pre- or post-contrast enhanced CT scans (sensitivity of 66%). Sarcomas differed in that they were either hyper or isodense; none were hypodense. The area of increased density matched the tumor geometry and correlated with dense cellularity and reticulin deposition. All 13 sarcomas showed contrast enhancement (100% sensitivity), but in two tumors, contrast enhanced CT underestimated the size of the tumor. Because of the large size and multiplicity of the sarcomas, HRUS imaging was not able to resolve the entire tumor volume because of limited imaging access. Intravenously injected horseradish peroxidase (HRP) crossed the tumor blood-brain barrier (BBB) only in those tumors in which contrast enhancement was seen. These studies suggest that intraoperative HRUS imaging may be useful in detecting and delineating human AA incompletely visualized by CT.

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Section: Comparison Of Ct Finding With Human Brain Tumorsmentioning

confidence: 57%

Correlation of neuropathologic findings, computerized tomographic and high-resolution ultrasound scans of canine avian sarcoma virus-induced brain tumors

et al. 1987

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“…Future investigations will aim to identify these substances emitting CL. There have been several reports of the identification of organic substances based on CL wavelength [25,26].…”

Section: Discussionmentioning

confidence: 99%

Dynamic autofluorescence imaging of intracellular components inside living cells using direct electron beam excitation

Nawa¹,

Inami²,

Miyake³

et al. 2014

Biomed. Opt. Express

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We developed a high-resolution fluorescence microscope in which fluorescent materials are directly excited using a focused electron beam. Electron beam excitation enables detailed observations on the nanometer scale. Real-time live-cell observation is also possible using a thin film to separate the environment under study from the vacuum region required for electron beam propagation. In this study, we demonstrated observation of cellular components by autofluorescence excited with a focused electron beam and performed dynamic observations of intracellular granules. Since autofluorescence is associated with endogenous substances in cells, this microscope can also be used to investigate the intrinsic properties of organelles.

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“…Although CL is essentially a similar phenomenon to conventional fluorescence elicited under ultraviolet light, it has some intrinsic advantages over the latter (Bröcker and Pfefferkorn, 1979;Pfefferkorn et al, 1980): (1) CL can be amplified electronically and measured quantitatively; (2) the depth of focus and the resolution in the CL mode is much higher than in the conventional fluorescence microscope; and (3) some substances exhibit luminescence only under electron irradiation. Although many attempts (Basu, 1983;Bond et al, 1974;Boyde and Reid, 1983;Bröcker and Pfefferkorn, 1979;Cavellier and Berry, 1985;Cavellier et al, 1978;De Mets, 1974;De Mets and Lagasse, 1971;Herbst and Hoder, 1978;Hörl, 1972Hörl, , 1978Hurter et al, 1981;Pearce and Hays, 1966;Schmidt et al, 1975;Soni et al, 1975) were made to utilize CL in biology, its applications have been limited because CL from organic chemicals is a very weak signal and easily fades under high beam current (De Mets and Lagasse, 1971).…”

Section: Introductionmentioning

confidence: 97%

Analytical color fluorescence electron microscopy of adrenal cortex

Nakano

Koike

Ogawa

1997

Microsc. Res. Tech.

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Evaluation of vasogenic edema in experimental brain tumors by cathodoluminescence and fluorescence microscopy

Cited by 8 publications

References 12 publications

Correlation of neuropathologic findings, computerized tomographic and high-resolution ultrasound scans of canine avian sarcoma virus-induced brain tumors

Correlation of neuropathologic findings, computerized tomographic and high-resolution ultrasound scans of canine avian sarcoma virus-induced brain tumors

Dynamic autofluorescence imaging of intracellular components inside living cells using direct electron beam excitation

Analytical color fluorescence electron microscopy of adrenal cortex

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