Evaluation of various phenotypic methods for differentiation of Candida dubliniensis from Candida albicans

Jan, Abiroo; Bashir, Gulnaz; Altaf, Insha; Fomda, Bashir Ahmad; Hamid, Sabiya; Jan, Kownsar

doi:10.1016/j.mimet.2021.106400

Cited by 5 publications

(4 citation statements)

References 23 publications

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“…Our study highlights that around 92.79% of positivity were seen with YEPD ( Yeast Extract Peptone Dextrose) which correlates with a study conducted by KIM et al, 18 where demonstration of Germ tubes were seen within 30 minutes in YEPD at 39 0 C but after 60 min in serum at 37 0 C. Whereas study conducted by Bhumla et al, 19 showed 81.25% production of germ tube production with YEPD and another study carried out by Abiroo Jan et al, 20 showed 89.30% germ tube production but only in Candida albicans and no germ tube formation in candida dubliniensis.…”

Section: Discussionsupporting

confidence: 76%

Use of various substrate/ media for germ tube test (GTT) detection in Candida albicans and Candida dubliniensis from a tertiary care hospital

Kotgire,

Hatkar

2024

IJMMTD

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In recent years, non-judicious use of broad spectrum antibiotics, corticosteroids, antineoplastic agents, immune suppressive drugs, widespread increased use of bone marrow and organ transplant, and unreasonable use of indwelling catheters have led to an increased incidence invasive candidiasis (IC). The germ tube test (GTT) is a well established, inexpensive, rapid and easy to dispense test used to identify . So the present study was conducted to assess the reliableness of different media for GTT production in and . A total of 102 and 09 strains were obtained from various clinical samples received at Department of Microbiology from a teaching institute and hospital located at west- central part of Maharashtra for a period of nine months. Medium that were employed for production of germ tube in and C.dubliniensis in the present study were trypticase soy broth, YEPD (Yeast Extract Peptone Dextrose) broth, Muller-Hinton agar/broth and peptone water which were assessed and compared with pooled human sera routinely used for detection of germ tube. For both the isolates human pooled serum showed 96.39% positivity followed by YEPD (yeast extract peptone dextrose) broth with 92.79% positivity, Trypticase soy broth showed 80.18% positivity, whereas Muller Hinton broth produced 67.56% positivity and least positivity was seen with Peptone water where only 45.04% of germ tube induction was observed. So our study clearly highlights that YEPD broth is better serum free media for germ tube production. Moreover, this medium is available commercially, is more stable, reliable, effective, is non biohazardous, convenient and cost effective alternative.

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Section: Discussionsupporting

confidence: 76%

Use of various substrate/ media for germ tube test (GTT) detection in Candida albicans and Candida dubliniensis from a tertiary care hospital

Kotgire,

Hatkar

2024

IJMMTD

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“…However, these methods are not accessible everywhere. A recent study corroborated that colony color on HiCrome candida differential agar after 72 h of incubation or growth on xylose-based agar medium after 48 h incubation is an easy, accurate, and cost-effective method of differentiating C. dubliniensis from C. albicans [ 22 ]. A mass spectrometry identification approach of C. dubliniensis appears reliable [ 23 , 24 ]; however, more database reviews are warranted [ 25 , 26 ].…”

Section: Discussionmentioning

confidence: 90%

Candida dubliniensis fungemia in a patient with severe COVID-19: A case report

Kakehi

Hagiya

Iio

et al. 2022

Journal of Infection and Chemotherapy

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“…(23) A pesar de contar con una especificidad del 100 %, la prueba de Tubo Germinal muestra una sensibilidad del 87,1 %, lo que sugiere una capacidad moderada para detectar la presencia de células fúngicas en muestras en general. (26) Sin embargo, su atractivo radica en su costo accesible en comparación con los métodos fenotípicos y PCR, cuyos costos por aislamiento resultan considerablemente elevados; en contraste, la prueba de Tubo Germinal se destaca por su costo relativamente insignificante (41) , lo que la convierte en una opción frecuentemente utilizada.…”

Section: Discussionunclassified

Current Perspectives on Antifungal Resistance: Focus on Opportunistic Mycoses

Aguiar Muñoz,

Yauli Flores

2024

Salud, Ciencia y Tecnología

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Introducción: las micosis oportunistas, causadas por hongos en individuos con sistemas inmunológicos comprometidos, están en aumento, lo que plantea desafíos significativos en la atención médica. Este artículo revisa la resistencia antifúngica con el objetivo de destacar su creciente incidencia y la necesidad de nuevas estrategias terapéuticas y métodos de diagnóstico precisos. Se subraya la necesidad de protocolos de laboratorio estandarizados y la implementación de técnicas sensibles para una detección temprana y un tratamiento óptimo. Métodos: se realizó una revisión sistemática en PubMed, Scopus y Google Scholar; se utilizaron términos de búsqueda y operadores booleanos tales como "(Opportunistic Mycoses) AND (Antifungal Resistance)" y "(Antifungals) AND (Resistance Genes)". A su vez, se siguieron las directrices establecidas por la declaración PRISMA y se empleó el enfoque PICO. Resultados: se evaluaron 12 estudios, 58,33 % (n=7) evaluaron la resistencia antifúngica, mientras que el 41,67 % (n=5) abordaron aspectos como mecanismos de acción y genética. El 66 % de los estudios evaluaron la resistencia de especies de Candida spp., mientras que el 16,7 % analizaron resistencias de Aspergillus spp. resultando en los patógenos más estudiados. Fueron evaluados diversos genes de resistencia en todos los estudios; además se encontró que el fluconazol es el antifúngico más estudiado, seguido de la anfotericina B y el voriconazol. Conclusión: la identificación temprana de la resistencia antifúngica, el desarrollo de nuevas terapias y la estandarización de métodos diagnósticos son esenciales para mitigar su impacto en la salud pública.

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Evaluation of various phenotypic methods for differentiation of Candida dubliniensis from Candida albicans

Cited by 5 publications

References 23 publications

Use of various substrate/ media for germ tube test (GTT) detection in Candida albicans and Candida dubliniensis from a tertiary care hospital

Use of various substrate/ media for germ tube test (GTT) detection in Candida albicans and Candida dubliniensis from a tertiary care hospital

Candida dubliniensis fungemia in a patient with severe COVID-19: A case report

Current Perspectives on Antifungal Resistance: Focus on Opportunistic Mycoses

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