Evaluation of various compounds to inhibit activity of matrix metalloproteinases in the tear film of horses with ulcerative keratitis

Ollivier, Franck J.; Brooks, Dennis; Källberg, M. E.; Komàromy, András M.; Lassaline, Mary; Andrew, Stacy E.; Gelatt, Kirk N.; Stevens, Gary; Blalock, Timothy D.; Setten, Gysbert‐Botho van; Schultz, Gregory S.

doi:10.2460/ajvr.2003.64.1081

Cited by 80 publications

(116 citation statements)

References 39 publications

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“…25,31 Serum has also been used as a therapeutic agent in veterinary patients, particularly those with corneal malacia, wherein the antiproteinase activity of serum reduces enzymatic corneal stromal degradation. 32,33 To the authors' knowledge, however, no published reports exist of studies evaluating the effect of topically applied serum in dogs with SCCED. Therefore, the purpose of the multicenter, randomized, doublemasked clinical trial reported here was to assess the effects on corneal wound healing of topical application of undiluted serum as an adjunct to standard treatment in dogs with SCCED.…”

Section: Iqrmentioning

confidence: 99%

Effects of topically applied heterologous serum on reepithelialization rate of superficial chronic corneal epithelial defects in dogs

Eaton

Hollingsworth

Holmberg³

et al. 2017

javma

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OBJECTIVE To assess the effects of topical application of undiluted heterologous serum on time to corneal reepithelialization in dogs with superficial chronic corneal epithelial defects (SCCEDs). DESIGN Multicenter, randomized, double-masked, controlled clinical trial. ANIMALS 41 client-owned dogs. PROCEDURES After collection of baseline clinical and historical data, dogs were randomly assigned to receive topically applied undiluted heterologous serum (n = 22) or isotonic saline (0.9% NaCl) solution (19) along with tobramycin and atropine. Epithelial debridement (at all visits) and grid keratotomy (at visits 2, 3, and 4) of SCCEDs were performed. Ophthalmic examination including fluorescein application was performed once weekly for 4 weeks or until corneal reepithelialization. Clinicians and owners were masked to treatment group. RESULTS No differences in baseline data were detected between treatment groups. No difficulties with medication administration, noncompliance, or adverse reactions were noted. All SCCEDs in both groups healed by 4 weeks after treatment began. Median time to reepithelialization (2 weeks) was not significantly different between serum-treated and placebo-treated eyes. Irrespective of treatment group, median time to reepithelialization was not significantly different for Boxers versus non-Boxer breeds. Direct correlations were detected between time to reepithelialization and vascularization score at study entry, vascularization score at time of reepithelialization, and ulcer area at study entry in both groups. Time to reepithelialization was not correlated with age, sex, or duration of signs in either group. CONCLUSIONS AND CLINICAL RELEVANCE Topical application of undiluted heterologous serum was well tolerated by dogs with SCCEDs but, as an adjunct to standard treatment, did not reduce time to corneal reepithelialization.

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Section: Iqrmentioning

confidence: 99%

Effects of topically applied heterologous serum on reepithelialization rate of superficial chronic corneal epithelial defects in dogs

Eaton

Hollingsworth

Holmberg³

et al. 2017

javma

View full text Add to dashboard Cite

show abstract

“…A ceratite fúngica configura-se em afecção frequente em equinos, com potencial risco de perda visual (OLLIVIER et al, 2003;BROOKS, 2010). Dentre os diversos fatores aos quais se atribui a elevada incidência de afecções corneais em equinos, elencamse o tamanho do bulbo do olho, seu posicionamento proeminente e lateralizado e atividades físicas dos equinos (STRUBBE et al, 2000;BAKER et al, 2008;WADA et al, 2010).…”

Section: Desenvolvimentounclassified

“…A MMP-2 encontra-se presente em sua forma inativa em córneas normais e possui como função a degradação de fibras colágenas ocasionalmente danificadas. A MMP-9 encontra-se presente após danos corneais, induzindo à destruição de estruturas adesivas da membrana basal das células epiteliais, retardando, por conseguinte, a reepitelização corneal (OLLIVIER et al, 2003;BOVELAND et al, 2010). Em córneas ulceradas, a atividade das proteinases encontra-se significativamente aumentada no filme lacrimal pré-corneal (STRUBBE et al, 2000;OLLIVIER et al, 2004).…”

Section: Desenvolvimentounclassified

“…Essa enzima degrada colágenos do tipo III e IV, bem como alguns compostos da matriz extracelular, tais como laminina e fibronectina (BERMAN et al,1977;FINI et al, 1992;STRUBBE et al, 2000;OLLIVIER et al, 2003;BROOKS et al, 2004).…”

Section: Desenvolvimentounclassified

“…Em alguns casos, a associação do soro com EDTA pode ser necessária. A acetilcisteína, entretanto, é desencorajada por alguns autores por romper a estabilidade do filme lacrimal (OLLIVIER et al, 2003;OLLIVIER et al, 2004) Corticosteroides são totalmente contraindicados e não devem ser empregados, tampouco na redução cicatricial de uma úlcera micótica, pois o fungo pode permanecer latente no estroma. Esses medicamentos têm sido implicados no aumento da multiplicação fúngica ou na predisposição à ceratomicose (BROOKS & MATTHEWS, 2007).…”

Section: Tratamento Médicounclassified

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