Evaluation of two underwater biopsy probes for in situ collection of shark tissue samples

Robbins, William D.

doi:10.3354/meps310213

Cited by 10 publications

(9 citation statements)

References 27 publications

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“…Based on the ability of samples as small as 12 mg DW (= 49 mg WW) to provide consistent FA profiles, our study shows that sufficient tissue samples are collected by standard biopsy darts (e.g., Daly and Smale, 2013;Jaime-Rivera et al, 2013) including the small dart assessed by Robbins (2006) which obtained 6.6-122 mg of total tissue. Although not stated what proportion of these biopsies were muscle, the large quantity of tissue obtained (up to 122 mg WW) suggests that sufficient muscle can be p(MC) values determined by Nested PERMANOVA (freezer group nested within sampling location) with Monte Carlo simulation between binned freezer groups (1 = 0-3 months) (2 = 4-6 months) (3 = 7-9 months) (4 = 10-12 months) (5 = 13-15 months)(6 = 16-18 months)(7 = 19-21 months) (8 = 22-24 months), the primary fatty acids (FA) driving the significantly different groups determined by SIMPER percent contribution.…”

Section: Minimum Samples Sizementioning

confidence: 99%

“…Muscle biopsies of variable forms have previously been developed to collect samples for genetic and isotopic studies, e.g., punch biopsies (Robbins, 2006;Daly and Smale, 2013) or thickgauged needles (Baker et al, 2004). Based on the ability of samples as small as 12 mg DW (= 49 mg WW) to provide consistent FA profiles, our study shows that sufficient tissue samples are collected by standard biopsy darts (e.g., Daly and Smale, 2013;Jaime-Rivera et al, 2013) including the small dart assessed by Robbins (2006) which obtained 6.6-122 mg of total tissue.…”

Section: Minimum Samples Sizementioning

confidence: 99%

“…Instead, minimally invasive biopsy techniques are often employed to obtain tissue samples for biochemical studies (e.g., Hooker et al, 2001;Carlisle et al, 2012;Hussey et al, 2012). With the development of specialized biopsy probes (Reeb and Best, 2006;Robbins, 2006;Daly and Smale, 2013), tissue samples can be obtained from free-swimming marine organisms, reducing the stress and detrimental effects of the capture and release process, and enabling the increased use of FA analyses across a number of species, including threatened elasmobranchs Rohner et al, 2013;Every et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Assessing the Functional Limitations of Lipids and Fatty Acids for Diet Determination: The Importance of Tissue Type, Quantity, and Quality

et al. 2017

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Lipid and fatty acid (FA) analysis is commonly used to describe the trophic ecology of an increasing number of taxa. However, the applicability of these analyses is contingent upon the collection and storage of sufficient high quality tissue, the limitations of which are previously unexplored in elasmobranchs. Using samples from 110 white sharks, Carcharodon carcharias, collected throughout Australia, we investigated the importance of tissue type, sample quantity, and quality for reliable lipid class and FA analysis. We determined that muscle and sub-dermal tissue contain distinct lipid class and FA profiles, and were not directly comparable. Muscle samples as small as 12 mg dry weight (49 mg wet weight), provided reliable and consistent FA profiles, while sub-dermal tissue samples of 40 mg dry weight (186 mg wet weight) or greater were required to yield consistent profiles. This validates the suitability of minimally invasive sampling methods such as punch biopsies. The integrity of FA profiles in muscle was compromised after 24 h at ambient temperature (∼20 • C), making these degraded samples unreliable for accurate determination of dietary sources, yet sub-dermal tissue retained stable FA profiles under the same conditions, suggesting it may be a more robust tissue for trophic ecology work with potentially degraded samples. However, muscle samples archived for up to 16 years in −20 • C retain their FA profiles, highlighting that tissue from museum or private collections can yield valid insights into the trophic ecology of marine elasmobranchs.

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Section: Minimum Samples Sizementioning

confidence: 99%

Section: Minimum Samples Sizementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessing the Functional Limitations of Lipids and Fatty Acids for Diet Determination: The Importance of Tissue Type, Quantity, and Quality

et al. 2017

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“…Equivalent underwater techniques have only recently been developed for sharks using a biopsy probe fitted to a spear gun (Robbins 2006). The diver fires a spear-mounted probe at sharks underwater to remove a small core of tissue for molecular analysis; the sharks survive with only a small hole.…”

Section: Introductionmentioning

confidence: 99%

“…The diver fires a spear-mounted probe at sharks underwater to remove a small core of tissue for molecular analysis; the sharks survive with only a small hole. Opportunistic use of the shark biopsy probe on 2 larger teleost fish, Cheilinus undulatus (Ruppell, 1835) and Bulbometapon muricatum (Valenciennes, 1840), also proved successful (Robbins 2006). However, a technique has not been trialed for small-to medium-sized teleost fish (15 to 70 cm).…”

Section: Introductionmentioning

confidence: 99%

Assessment of an underwater biopsy probe for collecting teleost fish tissue samples

Evans

2008

Mar. Ecol. Prog. Ser.

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Enhancing insights into foraging specialization in the world's largest fish using a multi‐tissue, multi‐isotope approach

Wyatt

Matsumoto

Chikaraishi

et al. 2019

Ecological Monographs

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Intra‐species variability in foraging strategies may be common, which has significant implications for efforts to understand and manage enigmatic species like the whale shark Rhincodon typus. The ecological relevance of differences in tissue isotopes within and between individuals in the context of foraging however depends on understanding tissue turnover times and carbon (Δ13C) and nitrogen (Δ15N) discrimination, which can vary with physiology, metabolism, and diet quality. Here, we examine isotope dynamics in captive R. typus as a basis for enhanced ecological insights into wild populations of the world's largest fish and other enigmatic species. A variable diet, principally consisting of two krill (Euphausia pacifica and Euphausia superba) provided an average of 48 ± 20 MJ/d (mean ± SD), or 2.7 ± 1.3 times basal metabolic requirements. On this diet, in agreement with allometric relationships, large body sizes (3,000–4,000 kg) were matched by slow plasma and cartilage turnover rates (empirically derived as 9 months and 3 yr, respectively), which provide tissue‐specific limits on the timescales over which we can isotopically detect diet changes in this species. Average diet‐to‐tissue discrimination showed significant variation between tissues (plasma and cartilage), and among growing and fasting individuals (Δ13C range, 1.5 to 5.5‰; Δ15N range, −0.1 to 2.9‰). Assimilation rates increased with temperature and were higher for the smaller E. pacifica (15 ± 2 mm) than E. superba (48 ± 2 mm). Growth significantly lowered both Δ15Nplasma and Δ15Ncartilage, with inappetence markedly reducing Δ15Nplasma and Δ13Cplasma, as well as significantly altering blood biochemistry. Captive findings facilitated the first robust multi‐tissue growth‐ and nutrition‐corrected isotope analysis of a wild R. typus population, suggesting individual foraging specialization on low trophic level mid‐ocean or coastal prey. Long‐term fasting during ocean‐basin‐scale migrations may be common and such metabolic effects should be carefully quantified when isotopically assessing intra‐species foraging differences. The metabolically constrained multi‐tissue, multi‐isotope approach described can facilitate ecological insights that are indispensable for effective conservation and management of globally threatened, but poorly understood, species by identifying differences in key foraging areas and target prey within and between individuals.

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Evaluation of two underwater biopsy probes for in situ collection of shark tissue samples

Cited by 10 publications

References 27 publications

Assessing the Functional Limitations of Lipids and Fatty Acids for Diet Determination: The Importance of Tissue Type, Quantity, and Quality

Assessing the Functional Limitations of Lipids and Fatty Acids for Diet Determination: The Importance of Tissue Type, Quantity, and Quality

Assessment of an underwater biopsy probe for collecting teleost fish tissue samples

Enhancing insights into foraging specialization in the world's largest fish using a multi‐tissue, multi‐isotope approach

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