Assessment of an underwater biopsy probe for collecting teleost fish tissue samples

Evans, Richard D.

doi:10.3354/meps07624

Cited by 8 publications

(4 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…87381 and 148534. Fish were captured using either baited, barbless hooks or on SCUBA with the use of biopsy probes attached to spears following Evans (). All individuals were identified to species level by trained observers and released alive at the capture site.…”

Section: Methodsmentioning

confidence: 99%

Validation of microsatellite multiplexes for parentage analysis and species discrimination in two hybridizing species of coral reef fish (Plectropomus spp., Serranidae)

Harrison

Feldheim

Jones

et al. 2014

Ecology and Evolution

View full text Add to dashboard Cite

Microsatellites are often considered ideal markers to investigate ecological processes in animal populations. They are regularly used as genetic barcodes to identify species, individuals, and infer familial relationships. However, such applications are highly sensitive the number and diversity of microsatellite markers, which are also prone to error. Here, we propose a novel framework to assess the suitability of microsatellite datasets for parentage analysis and species discrimination in two closely related species of coral reef fish, Plectropomus leopardus and P. maculatus (Serranidae). Coral trout are important fisheries species throughout the Indo-Pacific region and have been shown to hybridize in parts of the Great Barrier Reef, Australia. We first describe the development of 25 microsatellite loci and their integration to three multiplex PCRs that co-amplify in both species. Using simulations, we demonstrate that the complete suite of markers provides appropriate power to discriminate between species, detect hybrid individuals, and resolve parent–offspring relationships in natural populations, with over 99.6% accuracy in parent–offspring assignments. The markers were also tested on seven additional species within the Plectropomus genus with polymorphism in 28–96% of loci. The multiplex PCRs developed here provide a reliable and cost-effective strategy to investigate evolutionary and ecological dynamics and will be broadly applicable in studies of wild populations and aquaculture brood stocks for these closely related fish species.

show abstract

Section: Methodsmentioning

confidence: 99%

Validation of microsatellite multiplexes for parentage analysis and species discrimination in two hybridizing species of coral reef fish (Plectropomus spp., Serranidae)

Harrison

Feldheim

Jones

et al. 2014

Ecology and Evolution

View full text Add to dashboard Cite

show abstract

“…In most locations samples were collected using lethal spear fishing techniques. However, in the Whitsunday Islands a new tissue collection method was trialed successfully using an underwater biopsy probe to collect tissue samples (Evans, 2008).…”

Section: Collection and Sampling Designmentioning

confidence: 99%

Strong genetic but not spatial subdivision of two reef fish species targeted by fishers on the Great Barrier Reef

et al. 2010

View full text Add to dashboard Cite

“…In the GBR, samples were collected at seven locations from the Torres Strait in the north to the Capricorn Bunkers in the south (Figure 1 ). A total of 231 skin or fin‐clip samples of P. leopardus were collected between 2015 and 2019 using primarily nonlethal biopsy probes (method described by Evans, 2008 ) but also hook‐and‐line fishing and spear guns.…”

Section: Methodsmentioning

confidence: 99%

“…A total of 231 skin or fin-clip samples of P. leopardus were collected between 2015 and 2019 using primarily non-lethal biopsy probes (method described by Evans et al, 2008), but also hook-and-line fishing and spear guns.…”

Section: Samplingmentioning

confidence: 99%

Demographic histories shape population genomics of the common coral grouper (Plectropomus leopardus)

Payet

Pratchett

Saenz‐Agudelo

et al. 2022

Evolutionary Applications

View full text Add to dashboard Cite

Many coral reef fishes display remarkable genetic and phenotypic variation across their geographic ranges. Understanding how historical and contemporary processes have shaped these patterns remains a focal question in evolutionary biology, since they reveal how diversity is generated and how it may respond to future environmental change. Here we compare the population genomics and demographic histories of a commercially and ecologically important coral reef fish, the common coral grouper (Plectropomus leopardus [Lacépède 1802]), across two adjoining regions (the Great Barrier Reef; GBR, and the Coral Sea, Australia) spanning approximately 14 degrees of latitude and 9 degrees of longitude. We analysed 4,548 single nucleotide polymorphism (SNP) markers across 11 sites and show that genetic connectivity between regions is low, despite their relative proximity (~ 100 km) and an absence of any obvious geographic barrier. Inferred demographic histories using 10,479 markers suggest that the Coral Sea population was founded by a small number of GBR individuals and that divergence occurred ~ 190 kya under a model of isolation with asymmetric migration. We detected population expansions in both regions, but estimates of contemporary effective population sizes were approximately 50 % smaller in Coral Sea sites, which also had lower genetic diversity. Our results suggest that P. leopardus in the Coral Sea have experienced a long period of isolation that precedes the recent glacial period (~ 10 -120 kya) and may be vulnerable to localised disturbances due to their relative reliance on local larval replenishment. While it is difficult to determine the underlying events that led to the divergence of Coral Sea and GBR lineages, we show that even geographically proximate populations of a widely dispersed coral reef fish can have vastly different evolutionary histories.

show abstract

Assessment of an underwater biopsy probe for collecting teleost fish tissue samples

Cited by 8 publications

References 8 publications

Validation of microsatellite multiplexes for parentage analysis and species discrimination in two hybridizing species of coral reef fish (Plectropomus spp., Serranidae)

Validation of microsatellite multiplexes for parentage analysis and species discrimination in two hybridizing species of coral reef fish (Plectropomus spp., Serranidae)

Strong genetic but not spatial subdivision of two reef fish species targeted by fishers on the Great Barrier Reef

Demographic histories shape population genomics of the common coral grouper (Plectropomus leopardus)

Contact Info

Product

Resources

About