Evaluation of two rapid phenotypical tests—Alifax rapid AST colistin test and Rapid Polymyxin NP test—for detection of colistin resistance in Enterobacterales

Germ, Julija; Seme, Katja; Cerar, Tjaša; Hergouth, Veronika Križan; Pirš, Mateja

doi:10.1007/s10096-021-04182-w

Cited by 4 publications

(1 citation statement)

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“…Even though the test was designed specifically for colistin susceptibility testing on Enterobacterales, its performance was inferior to that of commercial BMD methods (Tables 1-2). Compared with other studies that have achieved a sensitivity of ≥ 97%, the overall performance of the in-house Rapid Polymyxin in this study (~92%) is slightly poor [17,18,24]. The CA of all the tests evaluated in this study except for CHROMAgar Col-APSE, is within the recommended standard (≥ 90%) for antimicrobial susceptibility testing systems.…”

Section: Discussioncontrasting

confidence: 58%

Evaluation of Six Commercial and Non-Commercial Colistin Resistance Diagnostics

Leshaba,

Mmatli,

Skosana

et al. 2024

Preprint

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Background. Resistance to colistin, a last-reserve antibiotic used for treating drug-resistant infections, is increasing globally. This study evaluated six diagnostic tests designed to detect colistin-resistant pathogens. Methods. PCR and broth microdilution assays (BMD) were used to respectively characterize the molecular mechanisms and phenotypic colistin resistance of 142 Gram-negative bacterial isolates and controls. The sensitivity, specificity, positive- and negative-predictive values, major (ME) and very major errors (VME), categorical and essential agreements (EA) of ComASP Colistin, CHROMagar COL-APSE, Rapid NP Test, Sensititre, MicroScan, and Vitek 2 were determined with these isolates; the BMD was used as gold standard. Results. The Vitek 2, Sensititre, and ComASP tests were more efficient, albeit with concerning ME and VMEs and low EAs. Sensititre was 100% specific with 0% ME and 3.61% VME; Vitek 2 had the least VME (1.25% and 0%) and a low EA (57.50%). ComASP had an EA of 75.35%. MicroScan was highly sensitive (96.55%) but less specific (87.50%), with very below-accetable EAs (48.11%). The CHROMAgar COL-APSE efficiently identified the species with their unique colours but was the least specific (67.80%), with the highest ME (32.20%) and high VME (7.23%). The Rapid NP test had the highest VME (7.84%), producing results within 4 hours with 92.16% sensitivity and 96.08% specificity. Conclusion: Vitek 2, MicroScan, ComASP colistin, and Sensititre are good for determining colistin resistance; the latter two tests are recommendable for low-resourced laboratories. The in-house Rapid NP test has short turnaround time with high efficiency for initial resistance screening.

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Section: Discussioncontrasting

confidence: 58%