Evaluation of two cotton varieties CRSP1 and CRSP2 for genetic transformation efficiency, expression of transgenes Cry1Ac+Cry2A, GT gene and insect mortality

Ali, Arfan; Ahmed, Shafique; Nasir, Idrees Ahmad; Rao, Abdul Qayyum; Ahmad, Saghir; Husnain, Tayyab

doi:10.1016/j.btre.2016.01.001

Cited by 17 publications

(11 citation statements)

References 27 publications

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“…A single copy number at chromosome 9 was detected through fluorescent in situ hybridization (FISH), while no signal was detected in non-transgenic control cotton plants (Figure 2). The results coincide with results of early researchers who determined transgene expression of Cry1ac and Cry2A along with GT gene in cotton and found a single copy number (Ali et al, 2016). This finding is consistent with a previous study in which overexpression of potato SuS in cotton under S7 promoter caused an increase in SuS activity and promoted fiber elongation (Xu et al, 2012).…”

Section: Discussionsupporting

confidence: 92%

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Overexpression of a Sucrose Synthase Gene Indirectly Improves Cotton Fiber Quality Through Sucrose Cleavage

et al. 2020

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The study aims to improve fiber traits of local cotton cultivar through genetic transformation of sucrose synthase ( SuS ) gene in cotton. Sucrose synthase (SuS) is an important factor that is involved in the conversion of sucrose to fructose and UDP-glucose, which are essential for the synthesis of cell wall cellulose. In the current study, we expressed a synthetic SuS gene in cotton plants under the control of a CaMV35S promoter. Amplification of an 813-bp fragment using gene-specific primers confirmed the successful introduction of SuS gene into the genome of cotton variety CEMB-00. High SuS mRNA expression was observed in two transgenic cotton plants, MA0023 and MA0034, when compared to the expression in two other transgenic cotton plants, MA0035 and MA0038. Experiments showed that SuS mRNA expression was positively correlated with SuS activity at the vegetative (54%) and reproductive stages (40%). Furthermore, location of transgene was found to be at chromosome no. 9 in the form of single insertion, while no signal was evident in non-transgenic control cotton plant when evaluated through fluorescent in situ hybridization and karyotyping analysis. Fiber analyses of the transgenic cotton plants showed increases of 11.7% fiber length, 18.65% fiber strength, and up to 5% cellulose contents. An improvement in the micronaire value of 4.21 was also observed in the MA0038 transgenic cotton line. Scanning electron microscopy (SEM) revealed that the fibers of the SuS transgenic cotton plants were highly spiral with a greater number of twists per unit length than the fibers of the non-transgenic control plants. These results determined that SuS gene expression influenced cotton fiber structure and quality, suggesting that SuS gene has great potential for cotton fiber quality improvement.

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Section: Discussionsupporting

confidence: 92%

“…Plants showing high expression of SuS were subjected to FISH for determination of copy number following the procedure published in earlier studies ( Ali et al, 2016 ; Yasmeen et al, 2016 ). Probe labeling was done by Label ITFISH ® labeling kit CY ® 3 (MIRUS cat # 6510) following the manufacturer’s instructions.…”

Section: Methodsmentioning

confidence: 99%

Overexpression of a Sucrose Synthase Gene Indirectly Improves Cotton Fiber Quality Through Sucrose Cleavage

et al. 2020

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“…Transformation efficiency of experiments was calculated to be 1.05% which is almost similar to the results obtained by Bakhsh et al (2012); Rao et al (2011). Amplification of 190bp fragment for GTGene and 1000bp for each of Cry1Ac + Cry2A confirmed successful transformation of three gene in cotton variety FBS-37 conferring resistance to the broadspectrum glyphosate and insects, respectively (Ali et al,(2016); Rao et al, 2013). The results of ELISA of Bt and GTGene in transgenic cotton plants confirmed their successful expression.…”

Section: Discussionsupporting

confidence: 72%

TRANSGENIC COTTON:HARBORING BROAD TERM RESISTANCE AGAINST INSECT AND WEEDS THROUGH INCORPORATION OF CEMB DOUBLE Bt AND cp4EPSPS GENES

Awan¹,

Ali²,

Muzaffar³

et al. 2016

PAKJAS

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“…al., 2000;Mahmood-ur-Rahman et al, 2010 and2014) of plants as well as in mammalian species. Visualizing or mapping of transgenes is important to determine their actual location on the chromosomes and their genetic analysis (Latif et al, 2015;Ali et al, 2016). FISH is an efficient method to construct physical maps of the genes regardless of the plant species.…”

Section: Introductionmentioning

confidence: 99%

Impact of Lycopene Supplemented Canola Meal-Based Diet on Growth Performance, Nutrient Digestibility and Antioxidant Status of Catla Catla Fingerlings

2021

THE JAPS

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The presence of Bt gene, cry1Ac in transgenic lines of Gossypium hirsutum L. was determined by molecular and cytogenetic analysis. Fluorescence in situ hybridization (FISH) was carried out to study the location of transgene on chromosomes. Metaphase chromosomes prepared from root tips and pollen grains showed more condensation, and hence more karyotypically differentiated. The five transgenic lines of cotton showed stable integration of transgene, cry1Ac. Either the lines were heterozygous (CEMB-3010-14, CEMB-3013-10, CEMB-3016-13 and CEMB-3037-2) or homozygous (CEMB-3034-10) showing single and double genes respectively at interphase and metaphase FISH while the control lines showed no fluorescent signal for transgene at all. Metaphase chromosomes prepared from pollens were also analyzed and single copy of cry1Ac was mapped on chromosome 10 which showed the stable inheritance of transgene in the progeny. The information obtained during study may be helpful to understand the possible effects of copy number variation and position effect of transgene in Bt cotton. The study may also help to comprehend the process of transgene inheritance in Bt cotton lines.

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Evaluation of two cotton varieties CRSP1 and CRSP2 for genetic transformation efficiency, expression of transgenes Cry1Ac+Cry2A, GT gene and insect mortality

Abstract: Graphical abstract

Cited by 17 publications

References 27 publications

Overexpression of a Sucrose Synthase Gene Indirectly Improves Cotton Fiber Quality Through Sucrose Cleavage

Overexpression of a Sucrose Synthase Gene Indirectly Improves Cotton Fiber Quality Through Sucrose Cleavage

TRANSGENIC COTTON:HARBORING BROAD TERM RESISTANCE AGAINST INSECT AND WEEDS THROUGH INCORPORATION OF CEMB DOUBLE Bt AND cp4EPSPS GENES

Impact of Lycopene Supplemented Canola Meal-Based Diet on Growth Performance, Nutrient Digestibility and Antioxidant Status of Catla Catla Fingerlings

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