Evaluation of Two Avian Influenza Type A Rapid Antigen Tests under Indonesian Field Conditions

Loth, Leo; Prijono, W.; Wibawa, Hendra; Usman, Tri Bhakti

doi:10.1177/104063870802000519

Cited by 17 publications

(35 citation statements)

References 12 publications

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“…The results are relevance to the report of Loth et al (2008) that specificity of rapid AIV Anigen R reaches 98%. According to the study of others rapid test kit that was Flu Detect TM strip antigen capture test has similar sensitivity of 98%.…”

Section: Sensitivity and Specificity Of The Testsupporting

confidence: 82%

“…The results of this study showed that the subtyping sensitivity was low (25%) compared to the similar research on rapid tests which have been conducted in Indonesia by Loth et al (2008). It was reported that the detection kit Anigen AIV rapid test kit had a higher sensitivity of 69%.…”

Section: Sensitivity and Specificity Of The Testmentioning

confidence: 48%

“…According to the study of others rapid test kit that was Flu Detect TM strip antigen capture test has similar sensitivity of 98%. Specificity tests of the rapid test conducted in Indonesia applied to sample obtained from AI infected chicken reported by Boland et al (2006) cited by Loth et al (2008) was reached 97%.…”

Section: Sensitivity and Specificity Of The Testmentioning

confidence: 98%

“…Rapid detection test of Flu DetectTM capture antigen test kit had a sensitivity of 71%. According to Boland et al (2006) and the report was cited by Loth et al (2008) stated that Anigen sensitivity reached 76%, for field application where samples obtained from AI infected chickens in Indonesia. Another study conducted by Chua et al (2007) showed that the sensitivity of the 5 types of rapid detection test kits for AI (brandname not specified) generally showed similar results which ranges from 36.3% to 51.4%.…”

Section: Sensitivity and Specificity Of The Testmentioning

confidence: 99%

“…However, viral titers generally are found in the trachea and cloaca of chicken higher than the duck (Chua et al 2008). According to Loth et al (2008) type of sample for the application of rapid test kit should be taken into consideration, particularly it should be obtained from the sick birds or birds that had just died due to AI virus infection. In principle, there should be enough virus titer in a sample for rapid positive results.…”

Section: Sensitivity and Specificity Of The Testmentioning

confidence: 99%

See 4 more Smart Citations

Evaluation of Rapid Detection Kit against Avian Influenza A Virus and H5 Subtype for Field Sample

Wibowo

Untari

Artanto

et al. 2017

Indones. J. Biotechnol.

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Avian influenza virus is a poultry viral disease, which causes high economic losses. Various efforts have been made to control the disease. One effort required fast and accurate screening diagnostic test. This study aimed to determine the potential of a rapid test kit, namely AIV/H5 Anigen Rapid Test for the detection of AI virus types A and subtype H5 in the fields. Some tests were carried out, e.g. the potential test, crossreaction test, sensitivity and specificity test. The potential test was done to evaluate detection limits of the kit, by having the test of a serial dilution of AI virus positive control. Crossreaction test was done to detect antigens other than AI virus H5N1, e.g. IB virus of Massachusetts strain, IBV strain 491, Newcastle Disease virus, and Escherichia coli. Sensitivity and specificity test were applied to the field samples which clinically and laboratory were confirmed as H5N1 positive. To confirm the result of rapid test was then being done by reverse transcriptase polymerase chain reaction. Based on these results it can be concluded that Anigen Kit AIV/H5 Ag Rapid Test can detect antigencontaining samples having AI virus HA titer up to 2 6 of type A virus, and up to 2 5 for subtype H5 virus. Anigen Kit AIV/H5 Ag Rapid Test showed no crossreactions with Infectious Bronchitis virus, Newcastle Disease virus, and Escherichia coli. Anigen A Rapid Test Kit AIV Ag showed a sensitivity of 50% and specificity of 100%, while Anigen Ag Rapid Test Kit AIV/H5 showed a sensitivity of 25% and specificity of 100%.

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Section: Sensitivity and Specificity Of The Testsupporting

confidence: 82%

Section: Sensitivity and Specificity Of The Testmentioning

confidence: 48%

Section: Sensitivity and Specificity Of The Testmentioning

confidence: 98%

Section: Sensitivity and Specificity Of The Testmentioning

confidence: 99%

Section: Sensitivity and Specificity Of The Testmentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of Rapid Detection Kit against Avian Influenza A Virus and H5 Subtype for Field Sample

Wibowo

Untari

Artanto

et al. 2017

Indones. J. Biotechnol.

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Rapid antigen detection in the diagnosis of highly pathogenic avian influenza (H5N1) virus in Nigeria

Meseko

Oladokun

Ekong

et al. 2010

Diagnostic Microbiology and Infectious Disease

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Identifying risk factors of highly pathogenic avian influenza (H5N1 subtype) in Indonesia

Loth¹,

Gilbert

et al. 2011

Preventive Veterinary Medicine

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Highly pathogenic avian influenza (HPAI), subtype H5N1, was first officially reported in Indonesia in 2004. Since then the disease has spread and is now endemic in large parts of the country. This study investigated the statistical relationship between a set of risk factors and the presence or absence of HPAI in Indonesia during 2006 and 2007. HPAI was evaluated through participatory disease surveillance (PDS) in backyard village chickens (the study population), and risk factors included descriptors of people and poultry distribution (separating chickens, ducks and production sectors), poultry movement patterns and agro-ecological conditions. The study showed that the risk factors “elevation”, “human population density” and “rice cropping” were significant in accounting for the spatial variation of the PDS-defined HPAI cases. These findings were consistent with earlier studies in Thailand and Vietnam. In addition “commercial poultry population”, and two indicators of market locations and transport; “human settlements” and “road length”, were identified as significant risk factors in the models. In contrast to several previous studies carried out in Southeast Asia, domestic backyard ducks were not found to be a significant risk factor in Indonesia. The study used surrogate estimates of market locations and marketing chains and further work should focus on the actual location of the live bird markets, and on the flow of live poultry and poultry products between them, so that patterns of possible transmission, and regions of particular risk could be better inferred.

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Evaluation of Two Avian Influenza Type A Rapid Antigen Tests under Indonesian Field Conditions

Cited by 17 publications

References 12 publications

Evaluation of Rapid Detection Kit against Avian Influenza A Virus and H5 Subtype for Field Sample

Evaluation of Rapid Detection Kit against Avian Influenza A Virus and H5 Subtype for Field Sample

Rapid antigen detection in the diagnosis of highly pathogenic avian influenza (H5N1) virus in Nigeria

Identifying risk factors of highly pathogenic avian influenza (H5N1 subtype) in Indonesia

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