Evaluation of triticale accessions for resistance to wheat bacterial leaf streak caused by <i>Xanthomonas translucens</i> pv<i>. undulosa</i>

Sapkota, Suraj; Zhang, Q.; Chittem, Kishore; Mohamed, Maryati; Xu, Steven S.; Liu, Z.

doi:10.1111/ppa.12768

Cited by 17 publications

(7 citation statements)

References 35 publications

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“…Virulence differences were also observed among strains within a pathovar (Cunfer and Scolari, ; Milus and Chalkly, ; Adhikari et al , ; Sapkota et al , ). Using pathogenicity tests on several wheat genotypes, Adhikari et al () found that X .…”

Section: Virulence and Pathogenicity Of X Translucensmentioning

confidence: 99%

“…undulosa (Bragard et al , ; Giblot‐Ducray et al , ; Curland et al , ). Using a few strains isolated from wheat and barley, we showed that wheat strains ( undulosa ) were capable of causing lesions and water‐soaking symptoms on wheat, barley, and triticale (× Triticosecale ), while barley strains ( translucens ) were only able to cause lesions and water‐soaking symptom on barley (Sapkota et al , ; Figure ). However, Curland et al () reported that most X. translucens pv.…”

Section: Classification Nomenclature and Identification Of X Transmentioning

confidence: 99%

“…Because no chemical control is available, host resistance appears to be the only way to control wheat BLS. Several sources of BLS resistance have been identified in diverse wheat or barley germplasm and related species (Hagborg, ; Akhtar and Aslam, ; Duveiller et al , ; Alizadeh et al , ; El Attari et al , ; Milus et al , ; Tillman et al , ; Adhikari et al , ; Kandel et al , ; Sapkota et al , ). These studies revealed a low percentage of resistant lines and no immune or highly resistant materials were found in the wheat germplasm.…”

Section: Genetics and Mapping Of Host Resistancementioning

confidence: 99%

“…These studies revealed a low percentage of resistant lines and no immune or highly resistant materials were found in the wheat germplasm. Nevertheless, several triticale lines were reported to possess high levels of BLS resistance (Cunfer and Scolari, 1982;Johnson et al, 1987;Sapkota et al, 2018).…”

Section: G Ene Ti C S and Mapping Of Hos T Re S Is Tan Cementioning

confidence: 99%

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The translucens group of Xanthomonas translucens: Complicated and important pathogens causing bacterial leaf streak on cereals

Sapkota

Mohamed

Liu

2020

Molecular Plant Pathology

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Xanthomonas translucens is a group of gram‐negative bacteria that can cause important diseases in cereal crops and forage grasses. Different pathovars have been defined according to their host ranges, and molecular and biochemical characteristics. Pathovars have been placed into two major groups: translucens and graminis. The translucens group contains the pathovars causing bacterial leaf streak (BLS) on cereal crops such as wheat, barley, triticale, rye, and oat. In recent years, BLS has re‐emerged as a major problem for many wheat‐ and barley‐producing areas worldwide. The biology of the pathogens and the host–pathogen interactions in cereal BLS diseases were poorly understood. However, recent genome sequence data have provided an insight into the bacterial phylogeny and identification and pathogenicity/virulence. Furthermore, identification of sources of resistance to BLS and mapping of the resistance genes have been initiated. Taxonomy Kingdom Bacteria; Phylum Proteobacteria; Class Gammaproteobacteria; Order Xanthomonadales; Family Xanthomonadaceae; Genus Xanthomonas; Species X. translucens; translucens group pathovars: undulosa, translucens, cerealis, hordei, and secalis; graminis group pathovars: arrhenatheri, graminis, poae, phlei; newly established pathovar: pistaciae. Host range X. translucens mainly infects plant species in the Poaceae with the translucens group on cereal crop species and the graminis group on forage grass species. However, some strains have been isolated from, and are able to infect, ornamental asparagus and pistachio trees. Most pathovars have a narrow host range, while a few can infect a broad range of hosts. Genome The complete genome sequence is available for two X. translucens pv. undulosa strains and one pv. translucens strain. A draft genome sequence is also available for at least one strain from each pathovar. The X. translucens pv. undulosa strain Xt4699 was the first to have its complete genome sequenced, which consists of 4,561,137 bp with total GC content approximately at 68% and 3,528 predicted genes. Virulence mechanisms Like most xanthomonads, X. translucens utilizes a type III secretion system (T3SS) to deliver a suite of T3SS effectors (T3Es) inside plant cells. Transcription activator‐like effectors, a special group of T3Es, have been identified in most of the X. translucens genomes, some of which have been implicated in virulence. Genetic factors determining host range virulence have also been identified.

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Section: Virulence and Pathogenicity Of X Translucensmentioning

confidence: 99%

Section: Classification Nomenclature and Identification Of X Transmentioning

confidence: 99%

Section: Genetics and Mapping Of Host Resistancementioning

confidence: 99%

Section: G Ene Ti C S and Mapping Of Hos T Re S Is Tan Cementioning

confidence: 99%

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The translucens group of Xanthomonas translucens: Complicated and important pathogens causing bacterial leaf streak on cereals

Sapkota

Mohamed

Liu

2020

Molecular Plant Pathology

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“…There is increasing interest in developing treatment options to combat BLS, including efforts to develop resistant germplasm in wheat (Sapkota et al 2018), but currently no major resistance is available for growers. There are no available chemical treatments, and antibiotics can quickly drive the development of resistance when applied in the field (Sundin and Wang 2018).…”

Section: Introductionmentioning

confidence: 99%

rpfFis not required forX. translucenspv. undulosa pathogenesis

Heiden,

Butchacas,

Klass

et al. 2024

Preprint

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Bacterial cells self-coordinate via a mechanism called quorum sensing. In Xanthomonas species the gene rpfF encodes the quorum sensing autoinducer synthase. Xanthomonas species are divided into two main phylogenetic groups called Clade I and Clade II. The rpf quorum sensing system has been well studied in multiple Clade II Xanthomonas species and deletion of rpfF resulted in a major loss of virulence on susceptible hosts. However, the only Clade I Xanthomonas species in which the rpfF system was previously studied was in the sugarcane pathogen X. albilineans. In X. albilineans the rpf cluster plays a relatively small role in pathogenesis. Xanthomonas translucens pv. undulosa (Xtu) is a Clade I Xanthomonas species that causes bacterial leaf streak (BLS) and black chaff of wheat and barley and has increased as a concern in recent decades. Neither major resistance nor chemical treatments are available to prevent disease caused by Xtu. Interference with rpf bacterial quorum sensing systems has demonstrated some success in other systems. It was unknown whether BLS caused by Xtu could be prevented via quorum sensing interference. We found that Xtu encodes an rpfF homolog and we created an rpfF knockout mutant to study the role of the rpf system in Xtu. We found that the rpfF mutant was unaffected in its pathogenesis as it caused BLS symptoms and multiplied within wheat plants to the same levels as the wildtype strain. The Xtu rpfF mutant grew normally in lag and log phases in vitro, however it exhibited a shorter stationary phase and an early death phase in plant-derived media. The importance of RpfF in the Xtu life cycle is unknown, though it appears to carry out a role in population stability. Our research determined that rpfF is not a major Xtu pathogenicity factor. Therefore, we do not recommend the targeting of the rpf quorum sensing system as a preventative treatment for BLS of wheat.

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