Evaluation of tricalciumphosphate/ hydroxyapatite cement for tooth replacement: an experimental animal study

Jansen, John A.; Ruijter, J.E. de; Schaeken, H. G.; Planell, J. A.; Driessens, F. C. M.

doi:10.1007/bf00123447

Cited by 63 publications

(45 citation statements)

References 17 publications

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“…By comparing the defects filled with α-TCP cement with control defects, there was higher new bone formation in experimental cavities at 90 days, confirming the studies conducted by Jansen et al 23 and Kessler et al 24 In this same observation period, the mean rate of new bone formation of 26.84% in the β-TCP/HA group was higher than in the control group (18.94%), and this difference was statistically significant; and smaller than in the α-TCP group (30.32%), without statistical significance. Alpha-tricalcium phosphate (alpha-TCP) ceramic is a bioresorbable material that degrades in bone tissue after implantation, since it exhibits higher solubility than beta-tricalcium phosphate (beta-TCP) ceramics.The high solubility of alpha-TCP in an aqueous solution causes its transformation into hydroxyapatite (HA) through hydrolysis 28 .…”

Section: Discussionsupporting

confidence: 86%

Comparative histomorphometric analysis between α-Tcp cement and β-Tcp/Ha granules in the bone repair of rat calvaria

et al. 2011

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This study compared the effect of two bioceramics on the process of bone repair: α-tricalcium phosphate (α-TCP) cement and β-tricalcium phosphate hydroxyapatite particles (β-TCP/HA). Calvarial defects were created in 50 rats, divided into two groups (α and β/HA). Software was used at 7, 21, 60, 90 and 120 days to assess bone formation. Mean new bone formation rates were as follows: α group, 1.6% at 7 days, 5.24% at 21 days, 24% at 60 days, 30.21% at 90 days and 50.59% at 120 days; β/HA group, 1.94% at 7 days, 2.53% at 21 days, 12.47% at 60 days, 26.84% at 90 days and 38.82% at 120 days; control group, 0.15% at 7 days, 10.12% at 21 days, 15.10% at 60 days, 18.94% at 90 days, 48.50% at 120 days. Both materials are osteoconductive and biocompatible. Perhaps the larger rate of new bone formation observed in the α-TCP group, it also occurs in the β-TCP/HA group within a longer time period.

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Section: Discussionsupporting

confidence: 86%

Comparative histomorphometric analysis between α-Tcp cement and β-Tcp/Ha granules in the bone repair of rat calvaria

et al. 2011

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“…The combination of a self-setting nature, biocompatibility, lack of any by-products and a great potential for replacement by bones make calcium orthophosphate cements, concretes, pastes and putties very promising materials for clinical and medical applications. In addition, they can easily be used by bone remodeling cells for reconstruction of damaged parts of bones [102,103,226,392,[470][471][472]. The ability to be molded in place also is a very important property because these formulations can easily be delivered into the desired place and can be fitted perfectly with bone defects [103].…”

Section: Clinical and Medical Applicationsmentioning

confidence: 99%

Self-Setting Calcium Orthophosphate Formulations: Cements, Concretes, Pastes and Putties

Dorozhkin¹

2012

IJMC

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In early 1980s, researchers discovered self-setting calcium orthophosphate cements, which are a bioactive and biodegradable grafting material in the form of a powder and a liquid. Both phases after mixing form a viscous paste that after being implanted sets and hardens within the body as either a non-stoichiometric calcium deficient hydroxyapatite (CDHA) or brushite, sometimes blended with un-reacted particles and other phases. As both CDHA and brushite are remarkably biocompartible and bioresorbable (therefore, in vivo they can be replaced with a newly forming bone), self-setting calcium orthophosphate cements represent a good correction technique of non-weight-bearing bone fractures or defects and appear to be very promising materials for bone grafting applications. Besides, these cements possess an excellent osteoconductivity, molding capabilities, and easy manipulation. Nearly perfect adaptation to the tissue surfaces in bone defects and a gradual bioresorption followed by new bone formation are additional distinctive advantages of calcium orthophosphate cements. Besides, reinforced formulations are available; those are described as calcium orthophosphate concretes. Furthermore, formulations with high viscosity, such as pastes and putties are also known. The discovery of self-setting formulations has opened up a new era in the medical application of calcium orthophosphates; several commercial compositions have already been introduced as a result. Many more formulations are in experimental stages. In this review, an insight into the self-setting calcium orthophosphate formulations, as excellent biomaterials suitable for both dental and bone grafting applications, has been provided.

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“…Such cements may be resorbed, allowing their gradual replacement by host bone. [4][5][6] As carrier materials for biological factors, calcium phosphate ceramics have been shown to have good drug delivery properties for antibiotics and growth factors. 7,8 The addition of a growth factor to calcium phosphate cement (CPC) may improve the osteoconductive properties of this material.…”

Section: Introductionmentioning

confidence: 99%

Transforming growth factor-?1 incorporated during setting in calcium phosphate cement stimulates bone cell differentiationin vitro

Blom

Klein‐Nulend

Klein

et al. 2000

J. Biomed. Mater. Res.

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Growth stimulation of periimplant tissues by growth factors like transforming growth factor-beta1 (TGF-beta1) may increase the indication for and success of implant use. Calcium phosphate as a material for implants or for coating of implants is known for its good biologic interaction with bone. Therefore, calcium phosphate implants combined with TGF-beta1 might improve osseointegration. In this study we hypothesise that the addition of recombinant human TGF-beta1 (rhTGF-beta1) to calcium phosphate cement (CPC) affects the differentiation of bone cells growing on the cement layer. rhTGF-beta1 incorporated during setting in a CPC layer at 20 ng rhTGF-beta1/60 mg cement was found to be gradually released into tissue culturing medium leading to a 20% release after 24 h. Two cell populations were obtained from collagenase-treated fragments of adult rat long bones: preosteoblastic cells, which were released by the collagenase treatment, and osteoblastic cells, which grew from the collagenase-stripped bone fragments. Both cell populations were tested for their osteoblastic characteristic phenotype by measuring their alkaline phosphatase (ALP) activity after vitamin D treatment and cyclic AMP after parathyroid hormone stimulation. After preculture the cells were plated on a layer of CPC containing 0 (control), 10, or 20 ng rhTGF-beta1/60 mg CPC. Bone cell differentiation was analyzed after 10 days by measuring the ALP activity, as well as the protein content of the cell layer. Incorporation of rhTGF-beta1 in the CPC did not change the ALP activity in osteoblastic cells, but a significant (analyzed by multivariate analysis of variance) increase was observed in preosteoblastic cells. Incorporation of 10 ng of rhTGF-beta1 in 60 mg of CPC increased the ALP activity in preosteoblastic cells by threefold and 20 ng rhTGF-beta1/60 mg CPC increased it by fivefold. The total protein content was not affected by rhTGF-beta1 in either of the cell populations. We conclude that rhTGF-beta1 incorporated during setting in CPC stimulates the differentiation of preosteoblastic cells in vitro. These results provide a basis for further studies on the use of this combination as an implant material in vivo.

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Evaluation of tricalciumphosphate/ hydroxyapatite cement for tooth replacement: an experimental animal study

Cited by 63 publications

References 17 publications

Comparative histomorphometric analysis between α-Tcp cement and β-Tcp/Ha granules in the bone repair of rat calvaria

Comparative histomorphometric analysis between α-Tcp cement and β-Tcp/Ha granules in the bone repair of rat calvaria

Self-Setting Calcium Orthophosphate Formulations: Cements, Concretes, Pastes and Putties

Transforming growth factor-?1 incorporated during setting in calcium phosphate cement stimulates bone cell differentiationin vitro

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