Evaluation of Total Reactive Antioxidant Potential (TRAP) of Tissue Homogenates and Their Cytosols

Evelson, Pablo; Travacio, Marina; Repetto, Marisa G.; Escobar, Jorge; Llesuy, Susana; Lissi, E. A.

doi:10.1006/abbi.2001.2292

Cited by 248 publications

(125 citation statements)

References 31 publications

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“…19 A avaliação da capacidade antioxidante total (TRAP) pode fornecer informações a respeito da capacidade dos sistemas em resistir aos desequilíbrios do estresse oxidativo. 12 O método utilizado neste estudo permite medir a capacidade da amostra em agir como antioxidante frente a radicais livres formados. A capacidade apresentada pela amostra é comparada a um padrão, neste caso o Trolox (vitamina E hidrossolúvel).…”

Section: Discussionunclassified

“…A capacidade apresentada pela amostra é comparada a um padrão, neste caso o Trolox (vitamina E hidrossolúvel). 10,11,12 Dentre as substâncias antioxidantes medidas pela técnica, estão: a vitamina C, o ácido úrico e a glutationa, principais antioxidantes não enzimáticos hidrossolúveis do plasma. As substâncias são mensuradas em conjunto e não individualmente.…”

Section: Discussionunclassified

“…Essa dependência dos valores de TRAP com a concentração de um antioxidante em que a concentração é pouco relacionada com o estresse oxidativo limita a utilização dessa técnica como um índice de estado antioxidante no plasma. 12 Medidas diretas dos níveis de vitaminas e outros antioxidantes hidrossolúveis poderiam auxiliar na discussão desses dados.…”

Section: Tabelaunclassified

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Efeito de uma sessão de hemodiálise sobre o estresse oxidativo sistêmico de pacientes renais crônicos terminais

Bianchi¹,

Barp²,

Thomé³

et al. 2009

J. Bras. Nefrol.

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Objective: To assess the effect of a hemodialysis (HD) session on systemic oxidative stress of chronic renal patients. Methods and Results: The study comprised 17 patients (10 women and 7 men with mean age of 39.9 ± 13.5 years) undergoing hemodialytic treatment at the Unit of Nephrology of Hospital de Clínicas de Porto Alegre, and a control group formed by 18 healthy individuals (4 women and 14 men with mean age of 34.8 ± 10.1 years). Renal patients had blood samples withdrawn before and after HD session. Means were analyzed by Student t test (p < 0.05). In red blood cells, the activity of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) was assessed. In plasma, the non-enzymatic antioxidant system was assessed by measuring the total reactive antioxidant potential (TRAP). No significant differences were observed between the values of SOD and CAT before and after HD. Comparison with the control group showed a significant reduction in the activity of those enzymes. After HD session, a significant reduction in TRAP was observed. Oxidative damage to membrane lipids was assessed through chemiluminescence (CL), and the damage to proteins through carbonyl assay. No significant difference was observed in the values of CL and carbonyls after HD. However, when compared with the control group, a significant difference was observed, indicating a greater damage to membrane lipids and proteins in renal patients. Conclusions: Chronic renal patients on HD treatment show a reduction in the activity of antioxidant enzymes SOD and CAT, in addition to an increase in oxidative damage, when compared with healthy individuals. One HD session does not change those levels.

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Efeito de uma sessão de hemodiálise sobre o estresse oxidativo sistêmico de pacientes renais crônicos terminais

Bianchi¹,

Barp²,

Thomé³

et al. 2009

J. Bras. Nefrol.

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“…According to literature, patients with diabetes mellitus, cardiovascular disease and gastrointestinal disease, obesity and metabolic syndrome showed decreased level of TAS during chronic exposition to high levels of Hcy [59]. Hyperhomocysteinemia decreased antioxidant defenses, and increased lipid peroxidation in the duodenum of rats, characterizing a reliable oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Different pathways involved in the stimulatory effects of homocysteine on rat duodenal smooth muscle

et al. 2017

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Recent studies have confi rmed that hyperhomocysteinemia is associated with gastrointestinal diseases; however, the direct effect of homocysteine on gastrointestinal reactivity still remains unknown. The aim of this study was to demonstrate how homocysteine may affect nitric oxide mediated duodenal relaxation and whether cholinergic receptors and K + channels take part in stimulating motility, as well as to explore whether oxidative stress is associated with homocysteine-mediated effects. Experiments were carried out on male rats, body mass 250-300 g. Two groups of animals were treated by i.p. application of saline and D,L-Hcy (0.6 μmol/g bm). After 2h of incubation, the duodenal segments were prepared for biochemical analysis and contractile response measurements in an organ bath with Tyrode's solution. Effects of TEA (10 mmol/L) and L-NAME (30 μmol/L) on duodenal contractility in the presence of D,L-Hcy (0.6 μmol/g bm) were investigated. Elevated homocysteine levels seem to be of crucial importance for the deterioration of contractility through nitric oxide mediated relaxation, and, in part, by activation of K + channels. Hcy showed direct promuscarinic effects, since 30 min pretreatment of rat duodenum signifi cantly enhanced the contractile effect of increasing concentrations of ACh (10 -9 -10 -2 mol/L). Catalase activity, superoxide dismutase, glutathione peroxidase and the total antioxidant system were reduced while the thiobarbituric acid-reactive substances level was elevated. Our data showed a consistent profi le of gastrointestinal injury elicited by sulfur-containing amino acid-homocysteine. This could contribute to explain, at least in part, the mechanisms involved in human gastrointestinal diseases associated to hyperhomocysteinemia.

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“…Non-enzymatic antioxidant defences constitute a large number of molecules, including amino acids, peptides, proteins and vitamins, that have different reaction centres (e.g. phenols and thiols) with widely different hydrophobicities that allow the trapping of both hydrophobic and hydrophilic radicals (Evelson et al, 2001). Moreover, skim milk protects domestic animal sperm against cold-shock damage (Pagl et al, 2006).…”

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Effects of skim-milk supplementation on the quality and penetrating ability of boar semen after long-term preservation at 15 °C

Namula

Kodama

Tanihara

et al. 2014

Acta Veterinaria Hungarica

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This study investigated the effects of skim-milk supplementation on the quality and penetrating ability of boar semen preserved at 15 °C. When boar semen samples were preserved in Modified Modena extender supplemented with various concentrations (0, 7.5, 15, 30 and 50 mg/mL) of skim milk powder at 15 °C for 4 weeks, higher sperm motility and viability were observed in the case of 7.5 mg/mL skim-milk supplementation compared with the control group (0 mg/mL) during the preservation (P < 0.05). When in vitro matured oocytes were co-incubated with boar sperm that had been preserved in Modified Modena extender with three different concentrations (0, 7.5 or 15 mg/mL) of skim milk powder at 15 °C for two weeks, there were no apparent effects of skim-milk supplementation on the rates of fertilisation and development to blastocysts of oocytes after co-incubation. However, the monospermic fertilisation rate of sperm preserved with 15 mg/mL skim milk powder was higher (P < 0.05) than that of fresh non-preserved sperm, but did not differ among the preservation groups. The results indicate that the supplementation of Modified Modena extender with 7.5 mg/mL skim milk powder improves the motility and viability, but not the penetrating ability, of sperm after liquid preservation for at least two weeks.

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Evaluation of Total Reactive Antioxidant Potential (TRAP) of Tissue Homogenates and Their Cytosols

Cited by 248 publications

References 31 publications

Efeito de uma sessão de hemodiálise sobre o estresse oxidativo sistêmico de pacientes renais crônicos terminais

Efeito de uma sessão de hemodiálise sobre o estresse oxidativo sistêmico de pacientes renais crônicos terminais

Different pathways involved in the stimulatory effects of homocysteine on rat duodenal smooth muscle

Effects of skim-milk supplementation on the quality and penetrating ability of boar semen after long-term preservation at 15 °C

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