Efeito de uma sessão de hemodiálise sobre o estresse oxidativo sistêmico de pacientes renais crônicos terminais

Bianchi, Patrícia Dall'Agnol; Barp, Jaqueline; Thomé, Fernando Saldanha; Belló‐Klein, Adriane

doi:10.1590/s0101-28002009000300002

Cited by 7 publications

(12 citation statements)

References 33 publications

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“…This is probably the first evidence that the increase in this toxin during the uremic condition coincides with increased oxidative stress and neutrophil dysfunction in dogs. In uremic dogs, the increase in lipid peroxidation, estimated by plasma TBARS, associated with a decrease in plasma antioxidants (uric acid, albumin, and TAC) confirm that during uremia oxidative stress occurs, as has been described in humans , cats , and dogs .…”

Section: Discussionsupporting

confidence: 77%

Free p-Cresol Alters Neutrophil Function in Dogs

Bosco¹,

Pereira²,

Almeida³

et al. 2015

Artificial Organs

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To achieve a clearer understanding of the mechanisms responsible for neutrophil dysfunction recently described in dogs with chronic renal failure (CRF), the plasma concentrations of free p-cresol in healthy dogs (n = 20) and those with CRF (n = 20) were compared. The degree of correlation was determined between plasma levels of p-cresol and markers of oxidative stress and function of neutrophils in these dogs. The effect of this compound on oxidative metabolism and apoptosis was assessed in neutrophils isolated from 16 healthy dogs incubated in RPMI 1640 supplemented with p-cresol (0.405 mg/L) and compared with medium supplemented with uremic plasma (50%). To achieve this, the plasma concentration of p-cresol was quantified by liquid phase high-performance liquid chromatography. The neutrophil oxidative metabolism was determined using the probes hydroethidine and 2',7'-dichlorofluorescein diacetate and apoptosis was measured using Annexin V-PE by capillary flow cytometry. Compared with the healthy dogs, uremic dogs presented higher concentrations of free p-cresol, greater oxidative stress, and neutrophils primed for accelerated apoptosis. The free p-cresol induced in neutrophils from healthy dogs increased apoptosis and decreased reactive oxygen species production. We conclude that the health status presented during uremia concomitant with the increase in plasma free p-cresol can contribute to the presence of immunosuppression in dogs with CRF.

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Section: Discussionsupporting

confidence: 77%

Free p-Cresol Alters Neutrophil Function in Dogs

Bosco¹,

Pereira²,

Almeida³

et al. 2015

Artificial Organs

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“…The authors observed a decrease in serum total antioxidant capacity during the HD treatment, which was attributed to the dialytic removal of uric acid and ascorbic acid. Similarly, Bianchi et al [ 42 ] investigated the consequences of an HD session on the systemic oxidative stress of CKD patients, assaying serum samples with the total reactive antioxidant potential (TRAP) test; when pre- and post-HD sera of CKD patients were compared, it was observed a reduction in TRAP after one HD session.…”

Section: Discussionmentioning

confidence: 99%

“…Bianchi et al [ 42 ] assessed the oxidative damage of lipids in erythrocytes of CKD patients by chemiluminescence assays for LPO initiated by tert -butyl hydroperoxide (t-BOOH), which are sensitive and have been used to observe indirectly the previous oxidative stress and diminished endogenous antioxidants [ 55 , 56 ]. When chemiluminescence data from pre- and post-HD patients were compared, no differences were observed, but an increased chemiluminescence was observed when pre-HD patients were compared with healthy subjects, showing a state of oxidative stress in these patients.…”

Section: Discussionmentioning

confidence: 99%

Effects of uremic solutes on reactive oxygen species in vitro model systems as a possibility of support the renal function management

et al. 2015

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BackgroundIn view of the prevalence of oxidative stress in chronic kidney disease (CKD) patients, the loss of low-molecular-weight biomolecules by hemodialysis and the antioxidant potential of some uremic solutes that accumulate in CKD, we used in vitro model systems to test the antioxidant potential of the following uremic solutes: uric acid, hippuric acid, p-cresol, phenol, methylguanidine, L-arginine, L-tyrosine, creatinine and urea.MethodsThe in vitro antioxidant efficiencies of the uremic solutes, isolated or in mixtures, were tested with the following assays: i) ABTS radical cation decolorization assay; ii) hypochlorous acid (HOCl/OCl−) scavenging activity; iii) superoxide anion radical (O2•-) scavenging activity; iv) crocin bleaching assay (capture of peroxyl radical, ROO•); v) hydrogen peroxide (H2O2) scavenging activity.ResultsFour of the tested uremic solutes (p-cresol, phenol, L-tyrosine, uric acid) were effective antioxidants and their IC50 were found in three model systems: ABTS•+, HOCl/OCl− and crocin bleaching assay. In the 4-solutes mixtures, each one of the solute captured 12.5% for the IC50 of the mixture to ABTS•+ or HOCl/OCl−, exhibiting a virtually exact additive effect. In the 2-solutes mixtures, for ROO• capture, it was observed the need of more mass of uremic solutes to reach an IC50 value that was higher than the projected IC50, obtained from the IC50 of single solutes (25% of each, in the binary mixtures) in the same assay. In model systems for O2•- and H2O2, none of the uremic solutes showed scavenging activity.ConclusionsThe use of the IC50 as an analytical tool to prepare and analyze mixtures allows the determination of their scavenging capacities and may be useful for the assessment of the antioxidant status of biological samples under conditions of altered levels of the endogenous antioxidant network and/or in the employment and monitoring of exogenous antioxidant therapy.

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“…Blood was collected by venipuncture in the cubital vein after local asepsis with 70% alcohol solution, with metal-free vacuum tubes containing EDTA as the anticoagulant (Vacuntainer ® , BD). A colorimetric method was used for Hb determination using Drabkin's reagent forming methemoglobin cyanide (Bianchi, Barp, Thomé, & Belló-Klein, 2009). Results are expressed as g/dl.…”

Section: Sample Collection Hb Blood Lead and Znpp Determinationsmentioning

confidence: 99%

Zinc‐protoporphyrin determination by HPLC with fluorescence detection as a biomarker of lead effect in artisanal pottery workers

Santos

Bandeira

Bah

et al. 2020

Biomedical Chromatography

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Lead (Pb) exposure compromises heme synthesis by inhibiting ferrochelatase, forming zinc‐protoporphyrin (ZnPP). This study aims to validate a method for the determination of ZnPP by HPLC with fluorescence detection and apply this method to evaluate the extent of Pb exposure of artisanal pottery workers. Extraction procedures were tested using both nonacid and acid liquid–liquid extraction. The former presented a better chromatogram and recovery results. The validated method yielded a good resolution of ZnPP and its free form peaks with acceptable precision and accuracy. Total run time was 15 min and ZnPP peak retention time was 5.6 min. We applied this method to evaluate 39 potters (90% male), mean age 40 years (9–80). The medians (ranges) of blood lead, ZnPP and hemoglobin were 16.0 μg/dl (2.2–71.5), 12.6 μg/dl (4.6–279.8) and 15.1 g/dl (11.0–17.8), respectively. Significant differences were observed for blood lead according to gender, age range (>40 years), direct handling of lead oxide and years of occupation. Significantly higher levels of ZnPP were observed in male potters involved with lead glazing activity. The validated method was shown to be simple with one‐step nonacid extraction, good sensitivity, reproducibility and accuracy. Our data shows that these laborers are dangerously exposed to Pb, reflecting the effect on the heme synthesis.

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Efeito de uma sessão de hemodiálise sobre o estresse oxidativo sistêmico de pacientes renais crônicos terminais

Cited by 7 publications

References 33 publications

Free p-Cresol Alters Neutrophil Function in Dogs

Free p-Cresol Alters Neutrophil Function in Dogs

Effects of uremic solutes on reactive oxygen species in vitro model systems as a possibility of support the renal function management

Zinc‐protoporphyrin determination by HPLC with fluorescence detection as a biomarker of lead effect in artisanal pottery workers

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