Evaluation of tissue reaction to Aroeira (Myracrodruon urundeuva) extracts: a histologic and edemogenic study

Abstract

ObjectivesThis study evaluated subcutaneous tissue response to Aroeira (Myracrodruon urundeuva) extract employing edemogenic and histological analyses. Material and methodsTest groups consisted of aqueous and ethanolic Aroeira extracts and saline (control). For edema quantification, 18 rats received an intravenous injection of Evan's Blue. After 30 min, the extracts and saline were injected on the dorsum of the rats, which were then sacrificed after 3 and 6 h. Readings were performed in a spectrophotometer. For subcutaneous implantation, 30 rats received a polyethylene tube containing the extracts on their dorsum and then they were killed after 7 and 28 days. The samples were processed for histological analysis and evaluated with a light microscope. The inflammatory infiltrate was quantified. ResultsThere were no statistically significant differences between aqueous extract and saline groups in relation to edema quantification in the different periods (p>0.05). Ethanolic solution resulted in more edema independently of the experimental period (p<0.05). Histological analysis showed similar results on the 7-day period for the 3 groups. There was a notable reduction on inflammatory cell number for saline and aqueous extract groups at 28 days. ConclusionThe aqueous extract showed biocompatible properties similar to those of saline.

Viability of Human Gingival Fibroblast (FGH) Treated with Ethanolic "Aroeira" Extract (Myracrodruon urundeuva Allemão)

“…Different doses (extract concentration) might determine the effect in a positive, or negative manner. In general, low concentrations of "aroeira" extract do not promote cell death and are biocompatible in different experimental models (Nunes Jr. et al 2008;Machado et al 2012;Machado and Oliveira 2014), as confirmed by the present results. In the present study, the high values of cell viability at lower concentrations in 48 h could be an effect of some components of the extract, such as glucose molecules from tannins and flavonoid (Souza 2012;Machado et al 2015).…”

“…These in vitro tests employing cell culture are advantageous due to their simplicity/quickness, low costs (Freshney 2005), and control of the some experimental conditions (pH, CO 2 concentration, and levels of some molecules) (Schweikl and Schmalz 1996;De Deus et al 2009). In general, there are contradictory results involving "aroeira" due to tissue biocompatibility (Nunes Jr. et al 2008;Machado et al 2012) and the induction of apoptosis in cellular lineages (Ferreira et al 2011). However, comparision of these works with the present results showed some similarities between them.…”

Medicamentos Fitoterápicos na odontologia: evidências e perspectivas sobre o uso da aroeira-do-sertão (Myracrodruon urundeuva Allemão)

“…Sabemos ainda que as plantas contem inúmeros constituintes e seus extratos podem apresentar efeitos sinérgicos entre os diferentes princípios ativos devido à presença de compostos de classes ou estruturas diferentes contribuindo para a mesma atividade (Maciel et al, 2002). A integração destas áreas na pesquisa de plantas medicinais conduz um caminho promissor e eficaz para descobertas de novos medicamentos.…”

Myracrondruon urundeuva Allemo: Chemical composition, antioxidant activity, antimicrobial activity and inotropic effect

“…Chalcones and dihydrochalcones belong to the class of flavonoids which, apart from their antioxidant activity, are known for their ability to strengthen capillary walls, thus assisting circulation and helping to prevent and treat bruising, varicose veins, bleeding gums and nosebleeds. In vitro, catechins are involved in antimicrobial, antioxidant activity and anti-inflammatory effects in this species (Viana et al, 2003;Sá et al, 2009;Machado et al, 2012). Still when being reported on the use of aroeira in accent baths against vaginal itching, it is used to boil its shells by women in post-partum baths, such as cicatrizant and anti-inflammatory (Silvino, 2014).…”