Evaluation of tissue levels of Toll-like receptors and cytokine mRNAs associated with bovine periodontitis and oral health

Borsanelli, Ana Carolina; Lappin, David F.; Viora, Lorenzo; King, George; Bennett, David; Dutra, Iveraldo S.; Riggio, Marcello P.

doi:10.1016/j.rvsc.2018.04.008

Cited by 4 publications

(4 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, Ling et al (2015) observed an increase in proinflammatory Pg -related cytokines IL-8 and IL-1β from peripheral Nϕs in patients with ChP as compared with controls. Furthermore, in a bovine study, TLR-2/4 gene expression was upregulated in periodontal tissues of animals with periodontitis (Borsanelli et al 2018). The increased signaling responses to Pg LPS suggests a priming effect of ChP on peripheral innate immune cells, perhaps through upregulation of the TLR-2/4 receptors.…”

Section: Discussionmentioning

confidence: 99%

Systemic Immunologic Consequences of Chronic Periodontitis

et al. 2019

View full text Add to dashboard Cite

Chronic periodontitis (ChP) is a prevalent inflammatory disease affecting 46% of the US population. ChP produces a profound local inflammatory response to dysbiotic oral microbiota that leads to destruction of alveolar bone and tooth loss. ChP is also associated with systemic illnesses, including cardiovascular diseases, malignancies, and adverse pregnancy outcomes. However, the mechanisms underlying these adverse health outcomes are poorly understood. In this prospective cohort study, we used a highly multiplex mass cytometry immunoassay to perform an in-depth analysis of the systemic consequences of ChP in patients before ( n = 28) and after ( n = 16) periodontal treatment. A high-dimensional analysis of intracellular signaling networks revealed immune system–wide dysfunctions differentiating patients with ChP from healthy controls. Notably, we observed exaggerated proinflammatory responses to Porphyromonas gingivalis–derived lipopolysaccharide in circulating neutrophils and monocytes from patients with ChP. Simultaneously, natural killer cell responses to inflammatory cytokines were attenuated. Importantly, the immune alterations associated with ChP were no longer detectable 3 wk after periodontal treatment. Our findings demarcate systemic and cell-specific immune dysfunctions in patients with ChP, which can be temporarily reversed by the local treatment of ChP. Future studies in larger cohorts are needed to test the boundaries of generalizability of our results.

show abstract

Section: Discussionmentioning

confidence: 99%

Systemic Immunologic Consequences of Chronic Periodontitis

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Composition, accumulation and dysbiosis of the dental biofilm, together with the host immune-inflammatory response, are elements involved in the aetiology of periodontitis in ruminants [1, 2]. In the context of these mixed and complex infections it is essential to understand the ecological relationships present in dental biofilms, which would allow understanding of the interrelationships between the components of the microbiota and how changes within this community could contribute to the progression of disease [3].…”

Section: Introductionmentioning

confidence: 99%

Dental biofilm and its ecological interrelationships in ovine periodontitis

Borsanelli

Athayde

Agostinho

et al. 2021

Journal of Medical Microbiology

Self Cite

View full text Add to dashboard Cite

Introduction. Periodontitis, one of the most common oral disorders in sheep, is caused by a mixed and opportunistic microbiota that severely affects the health and welfare of animals. However, little is known about the ecological processes involved and the composition of the microbiota associated with the development of the disease. Hypothesis/Gap Statement. Using high-throughput sequencing of the 16S ribosomal RNA gene and network analysis it would be possible to discriminate the microbiomes of clinically healthy sheep and those with periodontitis and possibly identify the key microorganisms associated with the disease. Aim. The present study aimed to characterise the composition of dental microbiomes and bacterial co-occurrence networks in clinically healthy sheep and animals with periodontitis. Methodology. Dental biofilm samples were collected from ten sheep with periodontitis and ten clinically healthy animals. Bacteria were identified using high-throughput sequencing of the 16S ribosomal RNA gene. Results. The most prevalent genera in the dental microbiota of sheep with periodontitis were Petrimonas , Acinetobacter , Porphyromonas and Aerococcus . In clinically healthy animals, the most significant genera were unclassified Pasteurellaceae, Pseudomonas, and Neisseria. Fusobacterium was found at high prevalence in the microbiomes of both groups. The dental microbiota of sheep in the two clinical conditions presented different profiles and the diversity and richness of bacteria was greater in the diseased animals. Network analyses showed the presence of a large number of antagonistic interactions between bacteria in the dental microbiota of animals with periodontitis, indicating the occurrence of a dysbiotic community. Through the interrelationships, members of the Prevotella genus are likely to be key pathogens, both in the dental microbiota of healthy animals and those with periodontitis. Porphyromonas stood out among the top three nodes with more centrality and the largest number of hubs in the networks of animals with periodontitis. Conclusion. The dental biofilm microbiota associated with ovine periodontitis is dysbiotic and with significant antagonistic interactions, which discriminates healthy animals from diseased animals and highlights the importance of key bacteria, such as Petrimonas , Porphyromonas , Prevotella and Fusobacterium species.

show abstract

“…These results could indicate the presence of an inflammatory environment. In a recent study, increased levels of Toll-like receptors and inflammatory cytokines in periodontal tissue of cattle with periodontitis were evidenced, suggesting that a relevant microbial challenge could be involved in the development of bovine periodontitis (Borsanelli et al, 2018b).…”

Section: Discussionmentioning

confidence: 99%

“…This disease has been shown to be multifactorial and polymicrobial, affecting the protection and support structures of teeth, in addition to being linked to systemic and environmental factors (Döbereiner et al, 2000;Dutra and Borsanelli, 2022). In this sense, the bacterial biofilm adhered to the dental unit is recognized as the initiating agent, together with the host inflammatory and immune response (Dutra et al, 2000;Borsanelli et al, 2018b).…”

Section: Introductionmentioning

confidence: 99%

Dysbiosis and predicted function of dental and ruminal microbiome associated with bovine periodontitis

et al. 2022

Self Cite

View full text Add to dashboard Cite

Extensive cattle livestock is advancing in Amazonia and its low productivity, with consequent pressure to open new areas, is partly due to sanitary problems and, among them, the periodontal diseases, whose environmental triggers or modifying factors are unknown. In this study, we used high-throughput sequencing, network analysis and predicted functions to investigate the dental and ruminal microbiota of cattle raised in new livestock areas in the Amazon and identify possible keystone pathogens and proteins associated with the disease. Ninety-three genera were common in dental and ruminal fluid microbiomes and among them periodontal pathogens such as Fusobacterium, Prevotella, Porphyromonas and Actinomyces were recognized. Network analysis showed that dental microbiomes of clinically healthy animals tend to comprise a group of OTUs in homeostasis and when analyzed together, dental and ruminal fluid microbiomes of animals with periodontitis had almost twice the number of negative edges, indicating possible competition between bacteria and dysbiosis. The incisor dental and ruminal fluid microbiomes were dominated by a core community composed of members of the phyla Firmicutes and Bacteroidetes. Network results showed that members of the Prevotella genus stood out among the top five OTUs, with the largest number of hubs in the dental and ruminal microbiota of animals with periodontitis. Protein families linked to an inflammatory environment were predicted in the dental and ruminal microbiota of cattle with periodontitis. The dissimilarity between dental microbiomes, discriminating between healthy cattle and those with periodontitis and the identification of possible key pathogens, represent an important reference to elucidate the triggers involved in the etiopathogenesis of bovine periodontitis, and possibly in the development of measures to control the disease and reduce the pressures for deforestation.

show abstract

Evaluation of tissue levels of Toll-like receptors and cytokine mRNAs associated with bovine periodontitis and oral health

Cited by 4 publications

References 40 publications

Systemic Immunologic Consequences of Chronic Periodontitis

Systemic Immunologic Consequences of Chronic Periodontitis

Dental biofilm and its ecological interrelationships in ovine periodontitis

Dysbiosis and predicted function of dental and ruminal microbiome associated with bovine periodontitis

Contact Info

Product

Resources

About