2021
DOI: 10.3389/fmicb.2021.643945
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Evaluation of Three Prokaryote Primers for Identification of Prokaryote Community Structure and Their Abode Preference in Three Distinct Wetland Ecosystems

Abstract: The ultimate role of prokaryote (bacteria and archaea), the decomposer of the wetland ecosystem, depends on its community structure and its interaction with the environment. The present study has used three universal prokaryote primers to compare prokaryote community structure and diversity of three distinctly different wetlands. The study results revealed that α-diversity indices and phylogenetic differential abundance patterns did not differ significantly among primers, but they did differ significantly acro… Show more

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Cited by 5 publications
(1 citation statement)
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References 127 publications
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“…MiniSeq platform was implemented by using primer sets 338F_806R and SSU0817F_1196R for 16S and 18S rDNA (Kumari et al, 2021), respectively to amplify relative genes in prokaryotic and eukaryotic microbes. The databases used for analyzing the rDNA data were silva 128/16S_bacteria and silva 128/18S_eukaryota respectively (http://www.arb-silva.de).…”
Section: Dna High Throughput Sequencingmentioning
confidence: 99%
“…MiniSeq platform was implemented by using primer sets 338F_806R and SSU0817F_1196R for 16S and 18S rDNA (Kumari et al, 2021), respectively to amplify relative genes in prokaryotic and eukaryotic microbes. The databases used for analyzing the rDNA data were silva 128/16S_bacteria and silva 128/18S_eukaryota respectively (http://www.arb-silva.de).…”
Section: Dna High Throughput Sequencingmentioning
confidence: 99%