Evaluation of three methods for enriching H2-producing cultures from anaerobic sludge

Mu, Yang; Yu, Han‐Qing; Wang, Gang

doi:10.1016/j.enzmictec.2006.07.033

Cited by 160 publications

(53 citation statements)

References 23 publications

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“…At the end of fermentation, the concentration of total VFAs in the four groups increased to 1137.1, 856.8, 856.6 and 1423.9 mg/L, respectively, and the fraction of acetate, propionate, butyrate and valerate in the four groups ranged from 40%-49%, 10%-15%, 14%-30% and 6%-31%, respectively. These results are different from those of hydrogen production from carbohydrate by heat or alkali pretreated seed sludge [13,18,19] and those from heat pretreated sludge by pure culture [11], in which VFAs mainly consisted of butyrate and acetate; but are similar to hydrogen production from the self-fermentation of pretreated sludge [21,22]. Due to the complicated substrate and microbial community, the pathway of hydrogen production from the self-fermentation of pretreated sludge was more complicated than that from carbohydrate [13,18,19] and by that of pure bacteria [11].…”

Section: The First Batch Tests With the Supernate Of Pre-treated Sludgementioning

confidence: 77%

“…For example, compared with alkali treatment, heat treatment can completely suppress the activity of methanogens [18]. Mu et al [19] stated that hydrogen production from glucose by heat-treated seed sludge was much higher than that by alkali-treated seed sludge. However, in another experiment using dairy wastewater, heat pretreated sludge obtained a very low hydrogen yield [20].…”

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confidence: 99%

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Impact of alkali and heat pretreatment on the pathway of hydrogen production from sewage sludge

2010

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Due to the presence of various types of hydrogen-producing bacteria and numerous organics such as protein and carbohydrate, sewage sludge is a potential material for biological hydrogen production. In this study, two batch tests were carried out to investigate the impact of alkali and heat pretreatment on the pathway of hydrogen production from sewage sludge. The results showed that the heat treatment had a stronger lethal effect on bacteria than the alkali treatment, and could effectively kill hydrogen-consuming bacteria. The heat treatment was more suitable for enriching acidophilic hydrogen-producing bacteria, while the alkali treatment was more suitable for enriching basophilic hydrogen-producing bacteria. A maximum hydrogen production of 10.32 mL/g-COD from alkali pretreated sludge was obtained at an initial pH of 11; while a maximum hydrogen production of 8.94 mL/g-COD from heat pretreated sludge was obtained at an initial pH of 5. Hydrogen production in alkali conditions (pH>9) from alkali pretreated sludge mainly depended on the fermentation of protein by protein-utilizing bacteria; whereas hydrogen production in acidic conditions (pH<6) from heat pretreated sludge mainly depended on the fermentation of carbohydrate by glucose-utilizing bacteria. sewage sludge, alkali pretreatment, heat pretreatment, hydrogen production Citation:Wei S Z, Xiao B Y, Liu J X. Impact of alkali and heat pretreatment on the pathway of hydrogen production from sewage sludge.

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Section: The First Batch Tests With the Supernate Of Pre-treated Sludgementioning

confidence: 77%

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confidence: 99%

Impact of alkali and heat pretreatment on the pathway of hydrogen production from sewage sludge

2010

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“…The improvements reported in these studies were attributed to the thermodynamic equilibrium of the hydrogen production process, as shown by free-Gibbs energy survey and analysis [10,15,46,54]. They were often directly linked to the hydrogen partial pressure, assuming that equilibrium was achieved in the bioreactors [14,55].…”

Section: Discussionmentioning

confidence: 99%

“…This mechanism allows the bacteria to continue their activity and growth by maintaining the pool of NADH/NAD + electron carriers and producing the ATP energy molecule. However, since these biochemical pathways are unfavourable for achieving maximum hydrogen production [4,14,15], several authors have proposed various techniques to decrease the concentration of metabolic gases soluble in the liquid phase [6].…”

Section: Introductionmentioning

confidence: 99%

Investigation of the links between mass transfer conditions, dissolved hydrogen concentration and biohydrogen production by the pure strain Clostridium butyricum CWBI1009

Beckers

Masset

Hamilton

et al. 2015

Biochemical Engineering Journal

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Fermentative hydrogen production has often been described as inhibited by its own gas production. In this work, hydrogen production by Clostridium butyricum was investigated in batch Biochemical Hydrogen Potential (BHP) tests and in a 2.5 L Anaerobic Sequenced Batch Reactor (AnSBR) under different operating conditions regarding liquid-to-gas mass transfer.Through the addition of both stirring up to 400 RPM and nitrogen sparging, the yields were enhanced from 1.6 to 3.1 mol H2 ·mol glucose -1 and the maximum hydrogen production rates from 140 to 278 mL·h -1 . These original results were achieved with a pure Clostridium strain. They showed that hydrogen production was improved by a higher liquid-to-gas hydrogen transfer resulting in a lower dissolved hydrogen concentration in the culture medium and therefore in a lower bacterial inhibition. In addition, biohydrogen partitioning between the gas and the liquid phase did not conform to Henry's Law due to critical supersaturation phenomena up to seven-fold higher than the equilibrium conditions. Therefore dissolved hydrogen concentration should be systematically measured instead of the headspace hydrogen partial pressure. A model was proposed to correlate H 2 production yield and rate by the pure C.butyricum strain CWBI1009 with mass transfer coefficient K L a.

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“…The inoculum used (10%v/v) was a digested sludge coming from the municipal wastewater treatment plant of Torino, chemically pretreated with HCl for 24 hours at pH 3 to inhibit the methane forming bacteria and enrich the H2 producing flora [39][40][41].…”

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confidence: 99%

Expression of different types of [FeFe]-hydrogenase genes in bacteria isolated from a population of a bio-hydrogen pilot-scale plant

Morra¹,

Arizzi²,

Allegra³

et al. 2014

International Journal of Hydrogen Energy

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production.An extensive analysis of the expression of [FeFe]-hydrogenases in the three best producer strains was achieved by RT-PCR, covering the complete set of known genes for each species.This revealed that during H2 production there are several different [FeFe]-hydrogenases simultaneously expressed, with genes belonging to the same phylogenetic and structural classification sharing similar transcriptional profiles.

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Evaluation of three methods for enriching H2-producing cultures from anaerobic sludge

Cited by 160 publications

References 23 publications

Impact of alkali and heat pretreatment on the pathway of hydrogen production from sewage sludge

Impact of alkali and heat pretreatment on the pathway of hydrogen production from sewage sludge

Investigation of the links between mass transfer conditions, dissolved hydrogen concentration and biohydrogen production by the pure strain Clostridium butyricum CWBI1009

Expression of different types of [FeFe]-hydrogenase genes in bacteria isolated from a population of a bio-hydrogen pilot-scale plant

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