Evaluation of three competitive ELISAs and a fluorescence polarisation assay for the diagnosis of bovine brucellosis

Praud, Anne; Durán-Ferrer, M.; Frétin, David; Jaý, Maryne; O'connor, Mary L.; Stournara, Athanasia; Tittarelli, Manuela; Dias, Isabel Travassos; Garin‐Bastuji, Bruno

doi:10.1016/j.tvjl.2016.06.014

Cited by 20 publications

(13 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was applied for the first time to diagnose bovine brucellosis in Albania in the current study. The analysis showed higher sensitivity and specificity compared to both the RBT and ELISA tests [27]. The test has been validated by many veterinary authorities [28].…”

Section: Discussionmentioning

confidence: 95%

See 1 more Smart Citation

The seroprevalence of brucellosis and molecular characterization of Brucella species circulating in the beef cattle herds in Albania

et al. 2020

View full text Add to dashboard Cite

Background Brucellosis is a ubiquitous zoonotic disease globally. It is endemic among bovines, sheep, and goats in Albania. The national control and eradication programs for brucellosis has been applied on sheep and goat farms as well as large dairy cattle farms, i.e., those with more than ten milking cows. The current study aims at estimating the herd and average individual animal prevalence of brucellosis in the national beef cattle herds, the missing information that was essential to propose the most appropriate control measures for this subpopulation. Rose Bengal Test (RBT), Fluorescence Polarization Assay (FPA), and Enzyme-Linked Immunosorbent Assay (ELISA) were used as serological tests and classical bacteriology for isolation. Results were also used to investigate the difference in sensitivity between the assays used.

show abstract

Section: Discussionmentioning

confidence: 95%

“…The analysis showed higher sensitivity and specificity compared to both the RBT and ELISA tests [27]. The test has been validated by many veterinary authorities [28]. It is standardized to be one of the main confirmatory tests for the diagnosis of brucellosis in cattle in most of the endemic countries of the Americas.…”

Section: Discussionmentioning

confidence: 99%

The seroprevalence of brucellosis and molecular characterization of Brucella species circulating in the beef cattle herds in Albania

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Indirect ELISA shows similar sensitivity and specificity to RBT in the absence of vaccination, but is more expensive [ 52 ] and need to be validated in the target population since its diagnostic performance depends on the manufacturer and the selection of an adequate cut-off to discriminate positive and negative samples [ 56 ] [ 42 ]. Despite original reports [ 57 ], recent studies show that competitive ELISAs do not have optimal sensitivity [ 58 ]. Thus, as Morocco is a country where no official brucellosis vaccination is currently practiced, sRBT and mRBT can be recommended for serological surveys in cattle and small ruminants respectively.…”

Section: Discussionmentioning

confidence: 99%

The prevalence of brucellosis and bovine tuberculosis in ruminants in Sidi Kacem Province, Morocco

et al. 2018

View full text Add to dashboard Cite

Bovine tuberculosis (BTB) and brucellosis are major endemic zoonoses in ruminants in Morocco that impact on both animal and human health. This study presents an assessment of the epidemiological and socioeconomic burden of bacterial zoonoses in Sidi Kacem Province in Northern Morocco from a cross-sectional survey of 125 cattle and/or small ruminant-owning households. In total, 1082 sheep and goats were examined from 81 households. The single intradermal comparative cervical test to screen for bovine tuberculosis was undertaken on 1194 cattle from 123 households and all cattle were blood sampled. Cattle and small ruminant sera were tested for brucellosis using the standard Rose Bengal Test (sRBT) and the modified Rose Bengal Test (mRBT). Bacteriology was performed on 21 milk samples obtained from cattle that were seropositive for brucellosis for isolation and phenotyping of circulating Brucella strains. Individual and herd prevalence for BTB in cattle of 20.4% (95% CI 18%-23%) and 57.7% (95% CI 48%-66%), respectively, were observed in this study. The prevalence of brucellosis in cattle at individual and herd level was 1.9% (95% CI 1.2%-2.8%) and 9% (95% CI 4.5%-1.5%), respectively. Brucella pathogens were isolated from three cattle milk samples and were identified as B. abortus using Bruceladder® multiplex PCR and B. abortus biovar 1 by classical phenotyping. All small ruminants were seronegative to sRBT, two were positive to mRBT. A higher risk of BTB and brucellosis was observed in cattle in intensive livestock systems, in imported and crossed breeds and in animals from larger herds (>15). The three risk factors were usually present in the same herds, leading to higher transmission risk and persistence of both zoonoses. These results highlight the importance of implementing control strategies for both BTB and brucellosis to reduce productivity losses and the risk of transmission to humans. Prioritising control for BTB and brucellosis in intensive livestock production systems is essential for human and animal health.

show abstract

“…It is assumed that the RB test has a sensitivity of 97.7% and a specificity of 99.9% (9). However, there are reports in the literature of much lower sensitivities ( Table 1).…”

Section: Introductionmentioning

confidence: 99%

Analytic Sensitivity of an ELISA Test on Pooled Sera Samples for Detection of Bovine Brucellosis in Eradication Stages in Uruguay

et al. 2020

View full text Add to dashboard Cite

Bovine brucellosis has been under eradication in Uruguay since 1998. The eradication program includes, among other interventions, individual sera sampling of beef animals at slaughter, and annual serum testing of all dairy cows-accounting for two million samples annually. At a herd prevalence of 0.8%, a pooled-sera sample approach could reduce the economic burden of the surveillance system by reducing the testing and operational costs. Our objective was to evaluate the analytic sensitivity of an indirect ELISA test for Brucella abortus in serum pools. Sixty-two Brucella abortus-positive bovine sera samples (based upon rose bengal and fluorescent polarization assay) were used as the positive control samples. Rose bengal-negative sera from negative farms were used to dilute the positive samples to the desired concentrations. Positive samples were diluted by using 1 ml of positive sera and 1 ml of negative sera (1/2 dilution) up to 1/1,024. Data were analyzed using generalized linear mixed models with a binary outcome (positive or negative), dilution number as a fixed effect, and a random effect for sample ID. Analytic sensitivity was 99.0% [95% confidence interval (CI): 96.3-99.7], 98.3% (95% CI: 93.1-99.6), 97.3% (95% CI: 87.4-99.4) for dilutions 1/2, 1/4, and 1/8, respectively. The analytical sensitivity, however, decreased when diluted to greater proportions. Given the current herd prevalence in Uruguay, it seems plausible that the use of a pooled sample approach could be adopted by policymakers to reduce the cost of the surveillance program and increase the number of samples being tested.

show abstract

Evaluation of three competitive ELISAs and a fluorescence polarisation assay for the diagnosis of bovine brucellosis

Cited by 20 publications

References 12 publications

The seroprevalence of brucellosis and molecular characterization of Brucella species circulating in the beef cattle herds in Albania

The seroprevalence of brucellosis and molecular characterization of Brucella species circulating in the beef cattle herds in Albania

The prevalence of brucellosis and bovine tuberculosis in ruminants in Sidi Kacem Province, Morocco

Analytic Sensitivity of an ELISA Test on Pooled Sera Samples for Detection of Bovine Brucellosis in Eradication Stages in Uruguay

Contact Info

Product

Resources

About