Evaluation of three Brucella soluble antigens used in an indirect Elisa to discriminate S19 vaccinated from naturally infected cattle

Abalos, Pedro; Daffner, J. F.; Pinochet, L.

doi:10.1016/s0378-1135(99)00141-8

Cited by 15 publications

(9 citation statements)

References 6 publications

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“…In addition, a small quantity is required for the screening of a large number of samples. However, by using LPS the specificity was slightly lower than the specificity (99.2%) observed by Abalos et al (2000) when using the same antigen. This may be due to a lower number of samples tested by I-ELISA and the use of polyclonal anti-bovine IgG in our studies.…”

Section: Discussionmentioning

confidence: 57%

Indirect Enzyme Linked Immunosorbent Assay for Diagnosis of Brucellosis in Buffaloes

Munir¹,

Rehman

Kausar³

et al. 2008

Acta Vet. Brno

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Brucellosis is an important zoonotic disease causing significant economic losses worldwide. Early detection of this disease is essential for its control and eradication. Presently, an Indirect Enzyme Linked Immunosorbent Assay (I-ELISA) was developed using lipopolysaccharide (LPS) as antigen and compared with the commercial kit using one hundred negative and positive sera each from buffaloes. The agreement for the positive result between the developed and commercial I-ELISA was 78% and for the negative it was 100%. At 52.49%, 53.09%, 53.26%, 53.86% and 53.94% cut off the sensitivity was 100%, 100%, 97.53%, 88.93% and 86.42%, while the specificity was 84.03%, 84.87%, 85.71%, 87.39% and 87.39%, respectively, for developed I-ELISA. This developed test can be used for the screening of herds as the relative sensitivity is higher. Brucella abortus, I-ELISA, lipopolysaccharides (LPS)

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Section: Discussionmentioning

confidence: 57%

Indirect Enzyme Linked Immunosorbent Assay for Diagnosis of Brucellosis in Buffaloes

Munir¹,

Rehman

Kausar³

et al. 2008

Acta Vet. Brno

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“…Finally, it is not restricted to bovine cattle and can be adapted to different species of animals as well as to humans. However, by using LPS the specificity was slightly lower than the sensitivity (99.2%) observed by Abalos et al (2000) when using the same antigen. According to Guarino et al (2001), the high percentage of positivity was due to the ability of this test to detect very low levels of antibodies present in the early stage of infection, while RBPT and SAT cannot detect it.…”

Section: Discussionmentioning

confidence: 61%

Seroprevalence of camel brucellosis (Camelus dromedarius) in Somaliland

Ghanem

El-Khodery

Saad³

et al. 2009

Trop Anim Health Prod

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The present study was delineated to investigate the prevalence and risk factors of camel brucellosis in Northern Somalia (Somaliland). The study was carried out at three main districts of camel-rearing regions of Somaliland (Awdal, Waqoyi Galbed and Togdheer) in the period from July to November, 2008. A total of 1246 camel blood sera were randomly collected from 42 sporadic small scale camel herds. Two serological tests were used to screen all serum samples, Rose Bengal Plate Test (RBPT) and indirect ELISA (I-ELISA). Multivariate logistic regression was constructed to study the risk factors associated with Brucella seropositive cases. The overall prevalence of camel brucellosis in districts under investigation was 3.9% by RBPT and 3.1% by (I-ELISA). Multivariate logistic regression on animal level showed that locality (P < 0.05; OR: 6.254; CI, 1.186-32.976), herd size (P < 0.001; OR: 5.493; CI, 2.956-10-207), rearing with other ruminants (P < 0.001; OR: 12.433; CI, 3.957-39.060), and contact with other camels (P < 0.05; OR: 5.311; CI, 1.093-25.800) were the potential risk factors. However, herd size (P < 0.05; OR: 5.425; CI, 1.181-24.932), and rearing with other ruminants (P < 0.05; OR: 20.466; CI, 1.456-28.638) were recorded as risk factors on the herd level. The results of the present investigation indicate that the Brucella spp. exists within the camel herds in Somaliland. Further studies need to be done on Brucella infection in the other ruminants to determine which measure should be followed for control of brucellosis.

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“…Different antigens including sLPS of Brucella bacteria are normally used in various IELISA development studies [2,5,6,8,9,[12][13][14][15]. The test protocol also varied between different studies [5,15,16].…”

Section: Resultsmentioning

confidence: 99%

“…In such studies, the sLPS Brucella abortus S1119-3 was used [2,5,14,15,17]. The sensitivity and specificity of this antigen were higher than other antigenic indices like Outer Membrane Protein (OMP 28 ) or native hapten (NH) [1,12].…”

Section: Resultsmentioning

confidence: 99%

Development of Indirect ELISA (IELISA) for Diagnosis of Bovine Brucellosis, Comparison of Three Different Labeled Detection Reagents

Mirjalili¹,

Lotfpouri²

2016

MOJI

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The aim of this study was to develop Indirect Enzyme-Linked Immunosorbent (IELISA) assay for diagnosis of bovine brucellosis and to compare the performance characteristics of developed IELISA by different kinds of enzyme labeled conjugate. Three IELISA tests were developed using Brucella abortus smooth lipopolysaccharide (sLPS) as coating antigen with three different enzyme labeled conjugates: the mouse monoclonal (mAb) anti-bovine IgG1, the polyclonal antibovine antibody and the recombinant A/G protein, all conjugated to Horseradish peroxidase enzyme were used for this study. More than 180 bovine serum samples which tested by conventional serological tests like Rose Bangal Test (RBT) and 2 Mercapto-Ethanol (2-ME) were used for assessment of performance characteristics of our developed IELISA. Cut-off value for three different enzyme labeled conjugates were OD 0.23 as calculated, the specificities and sensitivities of our three developed IELISA were 100%, 100% when mouse monoclonal anti-bovine IgG1 enzyme conjugate was used, 98.9, 100% once polyclonal antibovine labeled conjugate was used, and eventually 100%, 98.8% in those with recombinant A/G protein conjugate.

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Evaluation of three Brucella soluble antigens used in an indirect Elisa to discriminate S19 vaccinated from naturally infected cattle

Cited by 15 publications

References 6 publications

Indirect Enzyme Linked Immunosorbent Assay for Diagnosis of Brucellosis in Buffaloes

Indirect Enzyme Linked Immunosorbent Assay for Diagnosis of Brucellosis in Buffaloes

Seroprevalence of camel brucellosis (Camelus dromedarius) in Somaliland

Development of Indirect ELISA (IELISA) for Diagnosis of Bovine Brucellosis, Comparison of Three Different Labeled Detection Reagents

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