Evaluation of the wound-healing activity of Hibiscus rosa sinensis L (Malvaceae) in Wistar albino rats

Abstract

Objective:To investigate the wound-healing potency of the ethanolic extract of the flowers of Hibiscus rosa sinensis.Materials and Methods:The wound-healing activity of H. rosa sinensis (5 and 10% w/w) on Wistar albino rats was studied using three different models viz., excision, incision and dead space wound. The parameters studied were breaking strength in incision model, granulation tissue dry weight, breaking strength and collagen content in dead space wound model, percentage of wound contraction and period of epithelization in excision wound model. The granulation tissue formed on days 4, 8, 12, and 16 (post-wound) was used to estimate total collagen, hexosamine, protein, DNA and uronic acid. Data were analyzed by Analysis of Variance (ANOVA) test. P<0.05 was considered statistically significant.Results:The extract increased cellular proliferation and collagen synthesis at the wound site, as evidenced by increase in DNA, total protein and total collagen content of granulation tissues. The extract-treated wounds were found to heal much faster as indicated by improved rates of epithelialization and wound contraction. The extract of H. rosa sinensis significantly (P<0.001) increased the wound-breaking strength in the incision wound model compared to controls. The extract-treated wounds were found to epithelialize faster, and the rate of wound contraction was significantly (P<0.001) increased as compared to control wounds. Wet and dry granulation tissue weights in a dead space wound model increased significantly (P<0.001). There was a significant increase in wound closure rate, tensile strength, dry granuloma weight, wet granuloma weight and decrease in epithelization period in H. rosa sinensis-treated group as compared to control and standard drug-treated groups.Conclusion:The ethanolic extract of H. rosa sinensis had greater wound-healing activity than the nitrofurazone ointment.

EFEK EKSTRAK ETANOL 70% DAUN KEMBANG SEPATU (Hibiscus rosa-sinensis L.) DALAM MEMPERPENDEK WAKTU PERDARAHAN DAN WAKTU PEMBEKUAN PADA MENCIT JANTAN GALUR SWISS

“…Salah satu tanaman yang dimanfaatkan sebagai obat adalah kembang sepatu (Hibiscus rosa-sinensis L).Kembang sepatu (Hibiscus rosa-sinensis L.) termasuk familia malvaceae (Dalimartha, 2006).Manfaat dari kembang sepatu (Hibiscus rosa-sinensis L.)adalah sebagai antibakteri, antioksidan, antitumor, antihipertensi, dan sebagai penyembuh luka (Bhaskar & Nithya, 2012).…”

Importância do uso de plantas medicinais nos processos de xerose, fissuras e cicatrização na diabetes mellitus

“…No Brasil a DM atinge em torno de 7,6% da população entre 30 e 69 anos de idade, sendo que 50% dos pacientes desconhecem o diagnóstico e 24% não fazem qualquer tipo de tratamento (Gross & Nehme, 1999;Milman et al, 2001;Bortoletto et al, 2009).…”

“…Fatores de risco como a obesidade, o envelhecimento, mau controle glicêmico, tabagismo e hipertensão arterial sistêmica (HAS) contribuem para esse crescimento, chegando a mais de 87,9 milhões de reais ao ano, o custo das internações hospitalares com a doença na rede pública brasileira (SBACVRJ, 2013). As complicações comuns da DM são as lesões crônicas nos vasos sanguíneos (vasculopatias) e nos nervos (neuropatias) afetando principalmente rins, retina, artérias, cérebro e nervos periféricos (Gross & Nehme, 1999;Bortoletto et al, 2009).…”

“…(Gross & Nehme, 1999;Caiafa et al, 2011). Pacientes com história prévia de úlcera ou amputação têm elevado risco para o desenvolvimento de novas úlceras, infecção e gangrena, podendo culminar em amputação (Milman et al, 2001;Bortoletto et al, 2009;Marfella et al, 2012).…”

“…Fatores de risco como a obesidade, o envelhecimento, mau controle glicêmico, tabagismo e hipertensão arterial sistêmica (HAS) contribuem para esse crescimento, chegando a mais de 87,9 milhões de reais ao ano, o custo das internações hospitalares com a doença na rede pública brasileira (SBACVRJ, 2013). As complicações comuns da DM são as lesões crônicas nos vasos sanguíneos (vasculopatias) e nos nervos (neuropatias) afetando principalmente rins, retina, artérias, cérebro e nervos periféricos (Gross & Nehme, 1999;Bortoletto et al, 2009).Uma das principais causas de hospitalização de pacientes com DM é a amputação de membros inferiores, um fator de incapacidade, invalidez, aposentadoria precoce e morte que implica em altos custos financeiros. Geralmente, a amputação ocorre em decorrência das alterações vasculares e/ou neurológicas peculiares da doença, que são responsáveis pelo "pé diabético" (Milman et al, 2001).…”