Evaluation of the Utility of Serum Prolidase as a Marker for Liver Fibrosis

Stanfliet, John C.; Locketz, Michael; Berman, Peter; Pillay, Tahir S

doi:10.1002/jcla.21752

Cited by 6 publications

(3 citation statements)

References 37 publications

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“…Increased levels of prolidase activity are associated to some cancers, leading to the development of proline prodrugs (Mittal et al, 2005). Serum prolidase enzyme activity is also currently being explored as a biomarker for diseases including chronic hepatitis B and liver fibrosis (Duygu et al, 2013;Şen et al, 2014;Stanfliet et al, 2015). Phosphorylation of prolidase has been shown to increase its activity while dephosphorylation leads to a decrease in enzyme activity.…”

Section: -3-3 Protein Epsilon Isoform X1mentioning

confidence: 99%

Deiminated proteins and extracellular vesicles - Novel serum biomarkers in whales and orca

Magnadóttir

Uysal-Onganer

Kraev

et al. 2020

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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Section: -3-3 Protein Epsilon Isoform X1mentioning

confidence: 99%

Deiminated proteins and extracellular vesicles - Novel serum biomarkers in whales and orca

Magnadóttir

Uysal-Onganer

Kraev

et al. 2020

Comparative Biochemistry and Physiology Part D: Genomics and Pr

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“…In some cancers with increased levels of prolidase activity, such as melanoma, the differential expression of prolidase and its substrate specificity for dipeptides with proline at the carboxyl end suggests the potential of prolidase in becoming a viable, selective endogenous enzyme target for proline prodrugs (Mittal et al, 2005). Serum prolidase enzyme activity is also currently being explored as a biomarker for diseases including chronic hepatitis B and liver fibrosis (Duygu et al, 2013;Şen et al, 2014;Stanfliet et al, 2015). Phosphorylation of prolidase has been shown to increase its activity while dephosphorylation leads to a decrease in enzyme activity.…”

mentioning

confidence: 99%

Deiminated proteins in extracellular vesicles and serum of llama (Lama glama)—Novel insights into camelid immunity

Criscitiello

Kraev

Lange

2020

Molecular Immunology

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A B S T R A C TPeptidylarginine deiminases (PADs) are phylogenetically conserved calcium-dependent enzymes which posttranslationally convert arginine into citrulline in target proteins in an irreversible manner, causing functional and structural changes in target proteins. Protein deimination causes generation of neo-epitopes, affects gene regulation and also allows for protein moonlighting. Furthermore, PADs have been found to be a phylogenetically conserved regulator for extracellular vesicle (EVs) release. EVs are found in most body fluids and participate in cellular communication via transfer of cargo proteins and genetic material. In this study, post-translationally deiminated proteins in serum and serum-EVs are described for the first time in camelids, using the llama (Lama glama L. 1758) as a model animal. We report a poly-dispersed population of llama serum EVs, positive for phylogenetically conserved EV-specific markers and characterised by TEM. In serum, 103 deiminated proteins were overall identified, including key immune and metabolic mediators including complement components, immunoglobulin-based nanobodies, adiponectin and heat shock proteins. In serum, 60 deiminated proteins were identified that were not in EVs, and 25 deiminated proteins were found to be unique to EVs, with 43 shared deiminated protein hits between both serum and EVs. Deiminated histone H3, a marker of neutrophil extracellular trap formation, was also detected in llama serum. PAD homologues were identified in llama serum by Western blotting, via cross reaction with human PAD antibodies, and detected at an expected 70 kDa size. This is the first report of deiminated proteins in serum and EVs of a camelid species, highlighting a hitherto unrecognized post-translational modification in key immune and metabolic proteins in camelids, which may be translatable to and inform a range of human metabolic and inflammatory pathologies.

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“…[ 20 ] In previous studies, elevated serum prolidase activity has been associated with various diseases including diabetic neuropathy, liver fibrosis, bipolar disorder, anxiety disorder, and many other brain disorders. [ 40 41 42 43 ] Recently, Ozozen et al . have evaluated oxidative stress, inflammation, and the fibrotic process by measuring serum prolidase activity of adult with primary generalized epilepsy and they not found statistically significant differences in serum prolidase activity between patients with epilepsy and members of the healthy control group.…”

Section: Discussionmentioning

confidence: 99%

The serum prolidase enzyme activity as a biomarker for evaluation of the subclinical vascular damage in children with epilepsy

Karacan

Çalık

Kazanasmaz

et al. 2020

Ann Indian Acad Neurol

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Evaluation of the Utility of Serum Prolidase as a Marker for Liver Fibrosis

Abstract: Higher SPA levels in patients undergoing liver biopsies compared with controls may reflect the presence of liver fibrosis. SPA levels could not be used to stage the degree of fibrosis. SPA measurement may be useful in the diagnostic workup of suspected liver disease.

Cited by 6 publications

References 37 publications

Deiminated proteins and extracellular vesicles - Novel serum biomarkers in whales and orca

Deiminated proteins and extracellular vesicles - Novel serum biomarkers in whales and orca

Deiminated proteins in extracellular vesicles and serum of llama (Lama glama)—Novel insights into camelid immunity

The serum prolidase enzyme activity as a biomarker for evaluation of the subclinical vascular damage in children with epilepsy

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