Evaluation of the usefulness of immunofluorescence on Tzanck smears in pemphigus as an aid to diagnosis

Skeete, M.V.H.

doi:10.1111/j.1365-2230.1977.tb01538.x

Cited by 10 publications

(7 citation statements)

References 4 publications

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“…The specificity of direct immunofiuorescence for the detection of pemphigus antibodies in oral cytological smears has been a subject of controversy (Decherd et al, 1972, Skeete, 1977.…”

Section: Discussionmentioning

confidence: 99%

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Direct immunofluorescence on cytological smears in oral pemphigus

1981

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Direct immunofluorescence was performed on washed oral epithelial smears from thirteen patients with pemphigus vulgaris, thirteen patients with other oral diseases and from ten subjects with clinically healthy oral mucosa. The intercellular deposition of IgG was observed on cytological smears from oral smears from oral lesions in all patients with other oral diseases and from healthy controls, did not show any fluorescence. Therefore, direct immunofluorescence on cytological smears may be of value in the diagnosis of pemphigus.

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“…The specificity of direct immunofiuorescence for the detection of pemphigus antibodies in oral cytological smears has been a subject of controversy (Decherd et al, 1972, Skeete, 1977.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, intercellular deposition of IgG was demonstrated on cytological smears from oral pemphigus lesions (Decherd et ai, 1972). However, it was also reported that smears from other oral lesions (mainly aphthous ulcers) showed intercellular fluorescence similar to pemphigus (Skeete, 1977)-…”

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confidence: 99%

Direct immunofluorescence on cytological smears in oral pemphigus

1981

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“…Oral biopsies also give positive IMF results and are particularly useful in early cases of pemphigus vulgaris, because in the earliest stages of pemphigus a circulating IC antibody may be absent. Tzank smears are not satisfactory for IMF purposes; although specific fluorescence does occur around acantholytic cells in pemphigus, a similar degree of fluorescence may occur even in apthous ulceration (Skeete, 1977). Despite extensive acantholysis found in Hailey-Hailey disease and (Marsden et al, 1976) rifampicin (Gange et aL, 1976) transient acantholytic dermatosis, both the direct and the indirect IMF are consistently negative in these two disorders.…”

Section: Imf Findings In the Bullous Disordersmentioning

confidence: 99%

The value of immunofluorescence techniques in the diagnosis of bullous disorders: a review

1983

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Immunofiuorescence (IMF) techniques are often of considerable help in the diagnosis of bullous disorders. It must be emphasized however, that they do not circumvent the need for routine skin histopathology. It is by considering the results of IMF and histopathology together that one is likely to avoid making diagnostic errors. As with routine skin histopathology, if the initial IMF findings are negative and the bullous disease is evolving or changing its clinical appearance, both histopathology and IMF tests should be repeated at serial intervals. As with any laboratory or other diagnostic investigation, clinical judgement is at all times paramount in deciding on the correct diagnosis. Methods /. Specimens(a) Serum samples. About 10 ml of blood without anticoagulant should be collected and the serum separated from the clotted blood as soon as possible. Such serum samples can be mailed without freezing. Clotted whole blood should never be mailed frozen as this induces massive haemolysis.(b) Skin biopsy specimens. As in diagnostic histopathology the site of biopsy is of crucial importance. In the bullous dermatoses it is preferable to take the erythematous peri-bullous margin of early vesicles or bullae to use for IMF (Fig. t). At the same time it is convenient to excise the whole bulla in an eleptical biopsy so that the bulla and the other edge can be placed in formalin for routine histopathological examination. In general in our experience, punch biopsy

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“…Circulating intercellular antibodies, which are a hallmark of full-blown pemphigus vulgaris, may be lacking in OPV (Decherd et a1. 1972, Skeete 1977, Fiore-Denno and Samson 1979, Laskaris et a1. 1981) (2)(3)(4)7).…”

Section: Introductionmentioning

confidence: 99%

“…1972, Skeete 1977, Fiore-Denno and Samson 1979, Laskaris et a1. 1981) (2)(3)(4)7). Therefore, a diagnostic confirmation of the latter is based only on biopsy findings of involved mucosae, i. e., suprabasal acantholysis by histology and intercellular deposition of IgG by direct immunofluorescence.…”

Section: Introductionmentioning

confidence: 99%

Reliability of Cytodiagnosis in Oral Pemphigus Vulgaris

Ruocco¹,

Coscia-Porrazzi

Pisani³

1984

The Journal of Dermatology

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The aim of this study was to verify the reliability of the Tzanck test, performed by both traditional cytomorphology and the direct immunofluorescence technique, in diagnosis of oral pemphigus vulgaris. Cytologic smears were obtained from oral erosions of thirty patients with well ascertained pemphigus vulgaris. Control smears were taken from thirty patients affected by various other diseases of oral mucosa and from thirty healthy subjects. Cytologic diagnosis of oral pemphigus was based upon significant findings of acantholytic cells by cytomorphology, and of epithelial cells with pericellular deposition of IgG (which persisted after cytocentrifugation) by direct immunofluorescence. The cytomorphological test gave positive results in twenty‐eight patients with pemphigus and in one patient with herpetic stomatitis, and negative results in all other cases. The immunocytologic test gave positive results in all patients with pemphigus, and negative results in all controls. These findings indicate that the cytomorphological test may be a useful method for screening oral pemphigus, while the immunocytologic test may provide a reliable method for definitive diagnosis.

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Evaluation of the usefulness of immunofluorescence on Tzanck smears in pemphigus as an aid to diagnosis

Cited by 10 publications

References 4 publications

Direct immunofluorescence on cytological smears in oral pemphigus

Direct immunofluorescence on cytological smears in oral pemphigus

The value of immunofluorescence techniques in the diagnosis of bullous disorders: a review

Reliability of Cytodiagnosis in Oral Pemphigus Vulgaris

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