Evaluation of the use of low level laser and photosensitizer drugs in healing

Silva, Janine Cuzzolin E; Lacava, Z.G.M.; Kückelhaus, Selma Aparecida Souza; Silva, Luciano; Neto, Luis Ferreira Monteiro; Sauro, Evandro Emanoel; Tedesco, Antônio Cláudio

doi:10.1002/lsm.20062

Cited by 59 publications

(34 citation statements)

References 38 publications

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“…In the present study, the PBMT group showed an accelerated process of inflammation and proliferation that is in agreement with previous studies 36,37 . Furthermore, in the current study, the PBMT wounds showed an increase in the amount of collagen fibers and a more evident remodeling of connective tissue, and this corresponded with the results of the study where the PBMT at 685 nm with a dose 2.5 J/cm 2 was utilized for skin wound treatment 38 .…”

Section: Discussionsupporting

confidence: 89%

The effects of photobiomodulation therapy on Staphylococcus aureus infected surgical wounds in diabetic rats. A microbiological, histopathological, and biomechanical study

Ranjbar

Takhtfooladi

2016

Acta Cir. Bras.

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PURPOSE:To evaluate the effects of photobiomodulation therapy (PBMT) at 685 nm on diabetic wound healing in rats suffering from bacterial infection induced by Staphylococcus aureus (S. aureus). METHODS:Thirty streptozotocin-induced diabetic rats were allocated into two groups: control and PBMT. A 4-cm full-thickness linear-incision was made on the dorsal midline and was contaminated with S. aureus. The wounds in the PBMT group were irradiated daily for 5 consecutive days, starting 3 days after the induction and always in the mornings. RESULTS:The result revealed that PBMT resulted in a significant decrease in S. aureus CFU in the PBMT group in comparison to the control group (P<0.05). The length of wounds, in the 2nd and 3rd weeks, in the PBMT group were significantly shorter compared to the control group (P<0.05). PBMT caused a significant increase in the histological parameters in comparison to the control group (P<0.05). Moreover, PBMT significantly increased the breaking strength of the surgical scars produced in the skin of the PBMT group when compared to the control group (P<0.05). CONCLUSION:Photobiomodulation therapy may be useful in the management of wound infection through a significant bacterial growth inhibition and an acceleration of wound healing process.

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Section: Discussionsupporting

confidence: 89%

The effects of photobiomodulation therapy on Staphylococcus aureus infected surgical wounds in diabetic rats. A microbiological, histopathological, and biomechanical study

Ranjbar

Takhtfooladi

2016

Acta Cir. Bras.

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“…A limited number of reports suggest that bystander responses are also produced by other stresses, including chemotherapeutic drugs, (20) ultraviolet light (21–24) and photodynamic treatment (22, 25–28). PDT has been investigated for treatment of a variety of pathological conditions and diseases such as some cancers (29, 30), age-related macular degeneration (31), rheumatoid arthritis (32), skin conditions (33, 34) and wound healing (35, 36). PDT uses a combination of visible light and a localized photosensitizer to produce acute oxidative stress from singlet oxygen generation to eradicate diseased tissue.…”

Section: Introductionmentioning

confidence: 99%

Bystander Effects Induced by Diffusing Mediators after Photodynamic Stress

et al. 2009

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The bystander effect, whereby cells that are not traversed by ionizing radiation exhibit various responses when in proximity to irradiated cells, is well documented in the field of radiation biology, Here we demonstrate that considerable bystander responses are also observed after photodynamic stress using the membrane-localizing dye deuteroporphyrin (DP). Using cells of a WTK1 human lymphoblastoid cell line in suspension and a transwell insert system that precludes contact between targeted and bystander cells, we have shown that the bystander signaling is mediated by diffusing species. The extranuclear localization of the photosensitizer used suggests that primary DNA damage is not the trigger for initiating these bystander responses, which include elevated oxidative stress, DNA damage (micronucleus formation), mutagenesis and decreased clonogenic survival. In addition, oxidative stress in the bystander population was reduced by the presence of the membrane antioxidant vitamin E in the targeted cells, suggesting that lipid peroxidation may play a key role in mediating these bystander effects. The fluence responses for these bystander effects are non-linear, with larger effects seen at lower fluences and toxicity to the target cell population. Hence, when considering outcomes of photodynamic action in cells and tissue, bystander effects may be significant, especially at sublethal fluences.

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“…Moreover, in this study, in treated sites an increase in the amount of collagen fibres and an more evident remodeling (maturation phase) of connective tissue were shown, and this corresponded with the results of experiments were radiation from InGaAl P laser (685 nm) was used for acceleration of healing (Silva et al 2004). …”

Section: Discussionmentioning

confidence: 64%

Effects of diode laser therapy on the acellular dermal matrix

2009

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Acellular dermal matrix (ADM) was subcutaneously implanted into calvarian skin of male Wistar rats (n = 40). Low-level laser (lambda 685 nm, 4 J/cm(2)) was locally applied in experimental group (n = 20) above the skin flap. Grafts were harvested at 1, 3, 7 and 14 days after surgery and underwent histological analyses. In treated animals, the extent of edema and the number of inflammatory cells were reduced (P < 0.05). The amount of collagen in graft treated with low-level laser were significantly higher than those of controls (P < 0.05) and were statistically more prominent on the 14th day after surgery. The mean count of fibroblasts was significantly higher in the low-laser therapy group within the 3rd day, showing a marked influx of fibroblasts into area. In conclusion, wound healing of the ADM appear to be positively affected by laser therapy.

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Evaluation of the use of low level laser and photosensitizer drugs in healing

Abstract: The results clearly indicated a synergetic effect of light+photosensitizer+delivery drug on tissue healing. PDT did not cause any healing inhibition or tissue damage during the healing process.

Cited by 59 publications

References 38 publications

The effects of photobiomodulation therapy on Staphylococcus aureus infected surgical wounds in diabetic rats. A microbiological, histopathological, and biomechanical study

The effects of photobiomodulation therapy on Staphylococcus aureus infected surgical wounds in diabetic rats. A microbiological, histopathological, and biomechanical study

Bystander Effects Induced by Diffusing Mediators after Photodynamic Stress

Effects of diode laser therapy on the acellular dermal matrix

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