Evaluation of the use of free and immobilised acetylcholinesterase for paraoxon detection with an amperometric choline oxidase based biosensor

Cremisini, Carlo; Sario, Sabrina Di; Mela, J.; Pilloton, Roberto; Palleschi, Giuseppe

doi:10.1016/0003-2670(94)00618-v

Cited by 126 publications

(56 citation statements)

References 21 publications

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“…These results agree with previous reports [6,8]. At pH < 6.0, choline will lose its activity, and when pH > 8.0, the hydrolysis of organophosphates will occur.…”

Section: Optimum Experimental Condition For Choline Responsesupporting

confidence: 95%

Disposable Carbon Nanotube Modified Screen‐Printed Biosensor for Amperometric Detection of Organophosphorus Pesticides and Nerve Agents

2004

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A disposable carbon nanotube-based biosensor was successfully developed and applied to the detection of organophosphorus (OP) pesticides and nerve agents. The biosensors using acetycholinesterase (AChE)/choline oxidase (CHO) enzymes provided a high sensitivity, large linear range, and low detection limits for the analysis of OP compounds. Such characteristics may be attributed to the catalytic activity of carbon nanotube to promote the redox reaction of hydrogen peroxide produced during AChE/CHO enzymatic reactions with their substrate, as well as the large surface area of carbon nanotube materials.

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“…These results agree with previous reports [6,8]. At pH < 6.0, choline will lose its activity, and when pH > 8.0, the hydrolysis of organophosphates will occur.…”

Section: Optimum Experimental Condition For Choline Responsesupporting

confidence: 95%

Disposable Carbon Nanotube Modified Screen‐Printed Biosensor for Amperometric Detection of Organophosphorus Pesticides and Nerve Agents

2004

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“…The resulting profile showed maximum sensitivity of the biosensor at pH 7.0. This pH was chosen in previous reports in which free AChE was used [33,34]; and Mioetto et al observed an optimum pH range of 7.0 -8.0 for immobilized AChE enzyme [35]. It is evident from the experimental data that the QCdS-PVP does not alter the optimum pH for the catalytic behavior of cholinesterase and the microenvironment surrounding that the enzyme in the QCdS-PVP cages is easily accessed by the substrate in PBS.…”

Section: Ph Studiesmentioning

confidence: 87%

Development of Quantum Dots Modified Acetylcholinesterase Biosensor for the Detection of Trichlorfon

Xie

Min

et al. 2006

Electroanalysis

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Poly (N-vinyl-2-pyrrolidone) (PVP)-capped CdS quantum dots (QCdS-PVP) was synthesized with CdCl 2 and Na 2 S in the presence of PVP. QCdS-PVP has been used for the immobilization and stabilization of the acetylcholinesterase (AChE). The electrocatalytic activity of QCdS-PVP leads to a greatly improved electrochemical detection of the enzymatically generated thiocholine product, and higher sensitivity and stability. The GCE/QCdS-PVP/AChE biosensor was used for the detection of organophosphate pesticides (OPs), such as trichlorfon. The sensor performance, including pH and inhibition time, was optimized with respect to operating conditions. Under the optimal conditions, the biosensor was used to measure as low as 12 ppb trichlorfon with a 5-min inhibition time.

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“…São sensores do tipo impressos ou serigrafados, onde as camadas sub-seqüenciais eletródicas são depositadas sobre suportes finos, como cerâmica e plásticos, sendo que a enzima pode ser imobilizada sobre o eletrodo, em pequenas quantidades, por variadas metodologias, possibilitando tanto análises rápidas e baratas, quanto a aplicação in situ. [31][32][33][34][35][36] …”

Section: Materiais Empregadosunclassified

Biossensores baseados no processo de inibição enzimática

Marques¹,

Yamanaka²

2008

Quím. Nova

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Recebido em 3/5/07; aceito em 30/11/07; publicado na web em 4/9/08 BIOSENSORS BASED ON THE ENZYMATIC INHIBITION PROCESS. Fast, selective, reproducible and reliable detections have been carried out by using enzymatic biosensors in several areas. The enzymatic biosensors based on the inhibition represent an important role in analytical chemistry. Enzymes like cholinesterases, peroxidases, tyrosinases, etc. have been immobilized on electrochemical and optical transducers and the enzymatic activity decreasing in the presence of the inhibitor is related with its concentrations. This article presents a review on the enzymes used on the construction of these sensors, emphasizing the respective applications.

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Evaluation of the use of free and immobilised acetylcholinesterase for paraoxon detection with an amperometric choline oxidase based biosensor

Cited by 126 publications

References 21 publications

Disposable Carbon Nanotube Modified Screen‐Printed Biosensor for Amperometric Detection of Organophosphorus Pesticides and Nerve Agents

Disposable Carbon Nanotube Modified Screen‐Printed Biosensor for Amperometric Detection of Organophosphorus Pesticides and Nerve Agents

Development of Quantum Dots Modified Acetylcholinesterase Biosensor for the Detection of Trichlorfon

Biossensores baseados no processo de inibição enzimática

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