Evaluation of the toxicity of forb hay by biotesting method using <em>Paramecium caudatum</em>

Abstract

Today, the topic of assessing the toxicity of feed is relevant. In Russia, the production of environmentally friendly products is one of the priority areas in agriculture. Eating feed contaminated with industrial waste, pesticides, mycotoxins by domestic and farm animals can adversely affect their health. In this work, the quality of mixed grass hay was assessed by biotesting using Paramecium caudatum test organisms. The essence of the method lies in the preparation of aqueous extracts and aqueous solutions of acetone extracts of the sample and the effect of the obtained extracts on Paramecium caudatum. This method is convenient because you can quickly get the necessary results regarding the toxicity of the sample. The study was carried out on the basis of the laboratory of the Department of Biochemistry and Physiology of the St. Petersburg State University of Veterinary Medicine. Less than 50% of Paramecium caudatum survived in an aqueous extract and an aqueous solution of an acetone extract, the toxicity of the sample was established according to GOST 31674- 2012 «Feeds, compound feeds, material for compound feeds. Methods for the determination of common toxicity»