Evaluation of the toxicity of a methanolic total extract of &lt;i&gt;Mitragyna ciliata&lt;/i&gt; a natural anti-malaric

Bidie, Alain Dit Philippe; Koffi, Eugène; Yapi, FH; Yémié, A. A.; Djaman, Joseph Allico; Guédé-Guina, F.

doi:10.4314/ijbcs.v4i5.65558

Cited by 7 publications

(7 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Results in Laper and Placo did not differ significantly (P>0.05). These results are similar to those obtained by Bidie et al (2010) [33]. Indeed, these authors found an IC 50 of 7.5 μg / mL and 10.5 μg / mL in the extracts of Trichilia prieuriana and Mitragyna ciliata respectively.…”

Section: Discussionsupporting

confidence: 89%

See 1 more Smart Citation

Evaluation of Antioxidant Activities and Estimation of Zinc Content of Aqueous and Methanolic Extracts of Three Medicinal Plants: Cochlospermum Planchonii, Pericopsis Laxiflora and Harungana Madagascariensis

Jeanne¹,

Brice²,

Dieudonné³

et al. 2016

ijbb

View full text Add to dashboard Cite

Malaria disease is a pathology which occurs as a result of the oxidative stress. Three plants of the Ivorian pharmacopoeia are particularly used in the traditional medicine for the treatment of malaria in Côte d'Ivoire. They are Cochlospermum planchonii (Placo), Pericopsis laxiflora (Laper) and Harungana madagascariensis (Madhar).The objective of this study was to evaluate the in vitro antioxidant activity of the aqueous and methanolic extracts of three medicinal plants of Côte d'Ivoire. The quantitative measurement of total flavonoids gave the following results: Laper (560.80 mg QE/g ± 7.40), Madhar (352.50 mg QE/g ± 9.46) and Placo (314.20 mg QE/g ± 4.41). The total polyphenols were more concentrated in Laper (262.50 mg GAE/g ± 9.47) and Placo (203.20 mg GAE/g ± 2.21). Noticeable zinc content was found in Laper (13.56 ppm) and Placo (11.40 ppm) against 3.43 ppm in Madhar. The radical scavenging activity by 2,2-diphenyl-1-picryl hydrazyl (DPPH) and antiradical power measurement by ABTS (2,2' azino bis-3-ethylbenzothiazoline-6-sulphonic acid) assay have showed good activity for Laper (IC 50 = 7.50 µg/mL) and placo (IC 50 = 8.01 µg/mL). The standard antioxidant compound (quercetin) gave an IC 50 = 4.00 µg/mL. This study suggested that the high content of total flavonoids, total phenols and minerals in these plants could partly justify the therapeutic use of at least two of the three plants.

show abstract

Section: Discussionsupporting

confidence: 89%

“…50 g of drug powder were homogenized in 1.5 L of pure methanol in a mixer; the following steps were identical to those of the aqueous extraction above [26].…”

Section: Methanolic Extractionmentioning

confidence: 99%

Evaluation of Antioxidant Activities and Estimation of Zinc Content of Aqueous and Methanolic Extracts of Three Medicinal Plants: Cochlospermum Planchonii, Pericopsis Laxiflora and Harungana Madagascariensis

Jeanne¹,

Brice²,

Dieudonné³

et al. 2016

ijbb

View full text Add to dashboard Cite

show abstract

“…The method of Behrens and Karber (1983) was used for the calculation of the LD 50 which, generally, reflects the lethal nature of a substance (Bidie et al, 2010). The LD 50 values of H. madagascariensis extract were 11.6 g/kg and 13.2 g/kg for female and male mice respectively, whereas those of E. prostrata extract were 23.2 g/kg and 26.4 g/kg for female and male mice, respectively.…”

Section: Acute Toxicitymentioning

confidence: 99%

In vitro antimicrobial activity of Harungana madagascriensis and Euphorbia prostrata extracts against some pathogenic Salmonella sp.

Kengni¹,

Tala²,

Djimeli³

et al. 2013

Int. J. Bio. Chem. Sci

View full text Add to dashboard Cite

Harungana madagascariensis Lam (Hypericaceae) and Euphorbia prostrata Ait (Euphorbiaceae) are commonly used in Cameroon as traditional medicines for the treatment of typhoid fevers. Aqueous extracts were prepared from the leaves of H. madagascariensis and the whole plant of E. prostrata and tested in vitro for antibacterial activity against Salmonella Typhi, Salmonella Paratyphi A, Salmonella Paratyphi B and Salmonella Typhimurium, using broth dilution technique. Phytochemical screening was performed using standard methods. Acute toxicity study of the various extracts was also done on mice. Results obtained showed that H. madagascariensis extract exhibited minimum inhibitory concentrations (MICs) ranging from 390.625 to 1562.5 µg/ml. The median lethal dose (LD 50 ) of H. madagascariensis extract as shown by the acute toxicity studies were 11.6 g/kg and 13.2 g/kg body weight for female and male mice, respectively. The MIC values of E. prostrata extract varied from 1024 to 2048 µg/ml. The LD 50 values obtained for this extract were 23.2 g/kg and 26.4 g/kg body weight for female and male mice, respectively. Phytochemical analysis showed the presence of phenols, tannins, saponins, anthraquinones, anthocyanins, triterpenoids, flavonoids and alkaloids in both plant extracts. These data suggest that the aqueous extracts of H. madagascariensis and E. prostrata contain antibacterial principles which may be non toxic.

show abstract

“…Hepatic necrosis and oxidation have always been assessed through the measurement of markers like ASAT and ALAT (Tala et al, 2015;Olaleye et al, 2014;Bidie et al, 2010). In case of hepatotoxicity, the ALAT and ASAT increased simultaneously in the serum (Tala et al, 2015).…”

Section: Toxicity Testmentioning

confidence: 99%

Chemical composition, antibiotic promotion and in vivo toxicity of Piper nigrum and Syzygium aromaticum essential oil

Ismail¹,

Kemegne²,

Njayou³

et al. 2017

Afr. J. Biochem. Res.

View full text Add to dashboard Cite

The antimicrobial activity of spices of human daily diet has been widely demonstrated. Yet, their capacity of promoting antibiotic activity has not been explored adequately. In this work, the chemical composition of essential oils of Piper nigrum and Syzygium aromaticum from Chad were determined and their antimicrobial effect in combination with different antibiotics towards Staphylococcus aureus, Salmonella enteritidis and Salmonella typhi were assessed. Moreover, the in vivo toxicity of the essential oils alone and in combination with antibiotics was performed. Results indicate that the essential oil of P. nigrum was mostly composed of limonene (18.59%), beta-pinene (11.51%), linalool (10.17%), alpha-pinene (9.96%), while for S. aromaticum essential oil, only three compounds represented about 89% of the identified compounds. They were respectively beta caryophyllene (43.63%), eugenol (42.67%) and alpha-humulene (3.73%). Regarding the antimicrobial properties, Syzygium aromaticum essential oil (EO) used alone was more active than P. nigrum EO with a minimum inhibitory concentration (MIC) of 625 ppm towards all the strains. In combination with selected antibiotics, S. aromaticum gave better results with less indifference association, the best being with gentamicin. This association of the different EOs with gentamicin proved not to be acutely toxic to rats after 14 days of observation at doses ten times the MIC. These results indicated that spices used in human diets can be used for the production of hybrid drugs containing antibiotic and natural substances.Key words: Piper nigrum, Syzygium aromaticum, antibiotic promotion, Staphylococcus aureus, Salmonella, toxicity. INTRODUCTIONSpices are highly used in Chadian diet and many research works indicate that some of them have biological activities (Beuchat, 1994;Shan et al., 2007). In the continuous search for natural compounds with antimicrobial properties, a more safety approach is to use substances already belonging to human diet. Bacterial infection is one of the most current causes of illness worldwide (Kuete, 2013) and especially in developing countries (Adwan et al., 2008). According to Havelaar et al. (2015), food borne diarrheal disease agents, particularly non-typhoidal Salmonella enterica, cause about 23000 death worldwide, with a high percentage in Africa. In the same report, Salmonella Typhi was recognized as one of the major cause of food borne diseases. Regarding Staphylococcus aureus, Tong et al. (2015) stated that in the industrialized world, the population incidence of S. aureus bacteremia ranges from 10 to 30 per 100,000 person/year.Black pepper (Piper nigrum) has many medicinal properties among which, antibacterial, antifungal, antiinflamatory and antidiarrhoeal are mostly reported (Noumedem et al., 2013). Clove (Syzygium aromaticum) is an aromatic herb that has many useful purposes. Its essential oil has demonstrated several properties as reported by Noumedem et al. (2013). S. aromaticum is widely cultivated in Indonesia, Sri Lanka, Madagascar,...

show abstract

Evaluation of the toxicity of a methanolic total extract of <i>Mitragyna ciliata</i> a natural anti-malaric

Cited by 7 publications

References 15 publications

Evaluation of Antioxidant Activities and Estimation of Zinc Content of Aqueous and Methanolic Extracts of Three Medicinal Plants: Cochlospermum Planchonii, Pericopsis Laxiflora and Harungana Madagascariensis

Evaluation of Antioxidant Activities and Estimation of Zinc Content of Aqueous and Methanolic Extracts of Three Medicinal Plants: Cochlospermum Planchonii, Pericopsis Laxiflora and Harungana Madagascariensis

In vitro antimicrobial activity of <i>Harungana madagascriensis</i> and <i>Euphorbia prostrata</i> extracts against some pathogenic <i>Salmonella</i> sp.

Chemical composition, antibiotic promotion and in vivo toxicity of Piper nigrum and Syzygium aromaticum essential oil

Contact Info

Product

Resources

About