Evaluation of the Tissue Response to MTA and MBPC: Microscopic Analysis of Implants in Alveolar Bone of Rats

Cintra, Luciano Tavares Ângelo; Moraes, Ivaldo Gomes de; Estrada, Bernabé Pedro Felício; Gomes‐Filho, João Eduardo; Bramante, Clóvis Monteiro; Garcia, Roberto Brandão; Bernardinelli, Norberti

doi:10.1016/j.joen.2005.07.007

Cited by 54 publications

(75 citation statements)

References 35 publications

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“…The ability of the epoxy resin to seal and the biocompatibility of calcium hydroxide have been proven in vitro (6)(7)(8) and have shown good results in vivo (9).…”

Section: Introductionmentioning

confidence: 99%

L929 cell response to root perforation repair cements: an in vitro cytotoxicity assay

Miranda¹,

Fidel

Boller

2009

Braz. Dent. J.

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This study compared the cytotoxicity of an experimental epoxy-resin and calcium hydroxide-based cement (MBPc), gray mineral trioxide aggregate (MTA) and white mineral trioxide aggregate (WMTA) using the agar overlay method with neutral red dye. L929 cells were seeded into 6-well culture plates where 48-h set test materials were placed on the agar overlay, in triplicate. Teflon and natural rubber served as negative and positive controls. After an incubation period of 24 h at 37ºC in a humidified atmosphere of 5% CO 2 in air, a discolored area around the samples and the positive controls could be observed and measured per quadrant. The mean values were compared and converted into grades to classify the results according to the table of cytotoxicity grades according to the Standard Operating Procedures (SOP) of the Oswaldo Cruz Foundation, Brazil. The nonviable cell areas and the morphological changes in the cells were observed with an inverted microscope. The results showed grade 1 (slight) for the two types of MTA (p>0.05) and grade 2 (mild) for the MBPc (p<0.001). All samples met the requirements of the test as none of the cultures showed reactivity higher than grade 2.

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“…The ability of the epoxy resin to seal and the biocompatibility of calcium hydroxide have been proven in vitro (6)(7)(8) and have shown good results in vivo (9).…”

Section: Introductionmentioning

confidence: 99%

L929 cell response to root perforation repair cements: an in vitro cytotoxicity assay

Miranda¹,

Fidel

Boller

2009

Braz. Dent. J.

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“…Infiltration of a few inflammatory cells even in the control samples during the early period may be due to surgical trauma. Test bodies composed of endodontic materials have been implanted in rats [3][4][5][6][7]11,12) , rabbits 8,10) and guinea pigs 9) . The experimental tissue response to root canal-filling materials in dogs has suitably mimicked the clinical tissue response [13][14][15] .…”

Section: Introductionmentioning

confidence: 99%

A biocompatible model for evaluation of the responses of rat periapical tissue to a new zinc oxide-eugenol sealer

Mutoh

Tani‐Ishii

2011

Dent. Mater. J.

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We aimed to establish an experimental animal model to evaluate materials for endodontic therapy. We focused on the biocompatibility of new paste-type zinc oxide-eugenol (ZOE) sealer. The results of this sealer were compared with those of conventional powder/liquid ZOE and eugenol-free sealers. The molars of Wistar rats were extracted and repositioned in the original socket after application of the sealers on the root apices. Mild inflammation occurred in the periapical tissue of the replanted teeth with both ZOE sealers on day 7, whereas the eugenol-free sealer induced severe inflammation. On day 14, the lesions induced by all types of sealers were healed and replaced predominantly by fibrous connective tissue. Thus, all endodontic materials showed high biocompatibility, although the extent of inflammatory reactions during the early stages varied depending on the types of materials. We demonstrated that our animal model was useful for the assessment of the biocompatibility of endodontic materials.

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“…POLLICK, et al, 1995;CANOVA, 2003;SCHWARZ, et al, 2007), carneiros (GOSAIN, et al, 2002;), coelhos (MAEDA, 2005, camundongos (SCHWARTZ, et al, 2000), cobaias (RUMPEL, et al, 2006), hamsters (LASCHKE, et al, 2007) e os ratos de laboratório, que segundo Hedrich (2000), são os animais experimentais mais comumente utilizados, pois é o modelo que melhor representa o funcionamento do sistema mamífero. Serve como modelo para o teste de um grande número de materiais para aplicação em Endodontia e Cirurgia (TORNECK, 1966;PHILLIPS, 1967;TORNECK, 1967;HORSTED;FEJERSKOV;JOHANNESEN, 1980;GORDUYSUS;ETIKAN;GOKOZ, 1998;HOLLAND, et al, 1999;2001;2002a;2002b;MORAIS, et al, 2006;ZAMBUZZI, et al, 2006;GARCIA, 2008;GOMES, et al, 2008;MORI, et al, 2009 (MAGRO, et al, 1987;GORDUYSUS;ETIKAN;GOKOZ, 1998;YALTIRIK, et al, 2004;CINTRA, et al, 2006;MORAIS, et al, 2006;SUMER, et al, 2006;MORI, et al, 2009), de teflon (OLSSON;SLIWKOWSKI;ECONOMIDES, et al, 1995;KOLOKOURIS, et al, 1996;, ...…”

Section: 3-análise Estatísticaunclassified

“…Posteriormente, outros autores utilizaram também os tubos de polietileno, onde foi observada a ausência de inflamação frente ao material (MAGRO, et al, 1987;GORDUYSUS;ETIKAN;GOKOZ, 1998;YALTIRIK, et al, 2004;CINTRA, et al, 2006;MORAIS, et al, 2006;SUMER, et al, 2006;MORI, et al, 2009). Economides (1995), Kolokouris (1996; Holland et al (1999;2001;2002a;2002b) empregaram tubos de dentina preparados a partir de raízes de dentes humanos, para avaliação do MTA e cimento Portland em tecido subcutâneos de ratos.…”

Section: 11-dos Acondicionadores Empregadosunclassified

“…Em geral, os implantes dos materiais endodônticos, em tecido conjuntivo, são realizados em tubos de polietileno (MAGRO, et al, 1987;GORDUYSUS;ETIKAN;GOKOZ, 1998;YALTIRIK, et al, 2004;CINTRA, et al, 2006;MORAIS, et al, 2006;SUMER, et al, 2006;MORI, et al, 2009). São utilizados também tubos de teflon SLIWKOWSKI;ECONOMIDES, et al, 1995;KOLOKOURIS, et al, 1996;, de silicone rígido (ZMENER; GUGLIELMOTTI;…”

Section: Introductionunclassified

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Análise comparativa da reação tecidual à implantação de tubos de polietileno, bastões de dentina e cápsulas de colágeno. Estudo microscópico em tecido subcutâneo de ratos. Avaliação de um modelo metodológico

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Evaluation of the Tissue Response to MTA and MBPC: Microscopic Analysis of Implants in Alveolar Bone of Rats

Cited by 54 publications

References 35 publications

L929 cell response to root perforation repair cements: an in vitro cytotoxicity assay

L929 cell response to root perforation repair cements: an in vitro cytotoxicity assay

A biocompatible model for evaluation of the responses of rat periapical tissue to a new zinc oxide-eugenol sealer

Análise comparativa da reação tecidual à implantação de tubos de polietileno, bastões de dentina e cápsulas de colágeno. Estudo microscópico em tecido subcutâneo de ratos. Avaliação de um modelo metodológico

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