L929 cell response to root perforation repair cements: an in vitro cytotoxicity assay

Abstract

This study compared the cytotoxicity of an experimental epoxy-resin and calcium hydroxide-based cement (MBPc), gray mineral trioxide aggregate (MTA) and white mineral trioxide aggregate (WMTA) using the agar overlay method with neutral red dye. L929 cells were seeded into 6-well culture plates where 48-h set test materials were placed on the agar overlay, in triplicate. Teflon and natural rubber served as negative and positive controls. After an incubation period of 24 h at 37ºC in a humidified atmosphere of 5% CO 2 in air, a discolored area around the samples and the positive controls could be observed and measured per quadrant. The mean values were compared and converted into grades to classify the results according to the table of cytotoxicity grades according to the Standard Operating Procedures (SOP) of the Oswaldo Cruz Foundation, Brazil. The nonviable cell areas and the morphological changes in the cells were observed with an inverted microscope. The results showed grade 1 (slight) for the two types of MTA (p>0.05) and grade 2 (mild) for the MBPc (p<0.001). All samples met the requirements of the test as none of the cultures showed reactivity higher than grade 2.

Mineral Trioxide Aggregate: A Comprehensive Literature Review—Part II: Leakage and Biocompatibility Investigations

“…Another study compared GMTA with AWMTA in a L929 cell culture and reported that both types of MTA were superior in cytotoxicity to a CH base cement. The major drawback of the study was mixing both types of MTA in a 1:1 ratio of powder to liquid (179). Another cell culture investigation has shown that AMTA had the least cytotoxicity compared with some current pulpotomy agents such as Buckley's formocresol, CH, and ferric sulfate solution (180).…”

Evaluation of the cytotoxicity of polyurethane and non-latex orthodontic chain elastics

“…Cell lines [7], such as L929 mouse fibroblasts [8], have been shown to behave similarly to primary human gingival fibroblasts and, therefore, are a suitable in-vitro model to test the toxicity [9][10][11][12] of products used intra-orally during orthodontic treatment [13][14][15]. In order of evaluate the biological behavior of these materials in cell culture, the Objective of the present in vitro study was to test the cytotoxicity of polyurethane and latex-free Orthodontic elastomeric chains of different manufactures.…”

Citotoxicity of nonlatex elastomeric ligatures of orthodontic use

“…For other side, L929 mouse fibroblasts [8], have been shown to behave similarly to primary human gingival fibroblasts and, therefore, are a suitable in-vitro model to the toxicity test [9][10][11][12]. Taken in conjunction, these findings are aligned with the notion that the orthodontic elastics citotoxicity may influence their clinical performance [4,13] with regard to tissue biocompatibility.…”