Evaluation of the Spot-CAMP Test for the Rapid Presumptive Identification of Group B Streptococci

DiPersio, Joseph R.; Barrett, Jean E.; Kaplan, Raymond L.

doi:10.1093/ajcp/84.2.216

Cited by 7 publications

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“…In summary, the sensitivity of the spot CAMP test was 99%, and the specificity was 100%, with a predictive value of 100%7 for a positive test and 98.6% for a negative test. DiPersio et al (3) and others (5, 6, 8, 10) have had similar results in previous studies of the various CAMP tests. The spot CAMP reagent, prepared either commercially or in house, is stable for at least 1 year at refrigerator temperature (9) and can be made in the laboratory or purchased commercially.…”

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confidence: 81%

Evaluation of spot CAMP test for identification of group B streptococci

Ratner¹,

Weeks²,

Stratton³

1986

J Clin Microbiol

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The CAMP (Christie-Atkins-Munch-Petersen) test is commonly used for the presumptive identification of Streptococcus agalactiae (Lancefield group B). Using 350 clinical isolates of beta-hemolytic streptococci, we compared a 30-min spot CAMP test with the standard overnight CAMP test and the Lancefield precipitin test. We found 99% agreement among ail three tests for ail streptococci tested. The spot CAMP test is a rapid, inexpensive, and accurate method for identifying group B streptococci.

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supporting

confidence: 81%

Evaluation of spot CAMP test for identification of group B streptococci

Ratner¹,

Weeks²,

Stratton³

1986

J Clin Microbiol

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“…While this CAMP test is useful for the presumptive identification of pure cultures of suspected strains of GBS (9), the classical assay is not applicable for direct detection of GBS in primary cultures of clinical samples. Different modifications of the CAMP test have been developed for this purpose (3,10). However, none of these methods are optimal for routine examination of a large number of samples, since the procedures are relatively complicated.…”

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confidence: 99%

Method for Quantitative Detection and Presumptive Identification of Group B Streptococci on Primary Plating

Hansen

Sørensen

2003

J Clin Microbiol

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Maternal prenatal screening for group B streptococci (GBS) followed by offering of intrapartum chemoprophylaxis to carriers is one of the strategies used to reduce the incidence of neonatal early-onset GBS infections. Culturing of vaginal and anorectal swab specimens in selective broth is the screening procedure recommended by the Centers for Disease Control and Prevention. This technique is sensitive; it does not, however, allow either evaluation of the degree of colonization or detection of cocolonization with several GBS clones. We have examined the carriage rate and population dynamics of GBS in a group of Danish women during pregnancy and 1 year after delivery using a new detection method. In the present paper we describe a mixed blood agar medium (MB agar) that identifies GBS in the primary cultures by detection of a double hemolysis pattern consisting of characteristic, large zones of partial hemolysis ("CAMP zones") and of narrow zones of complete hemolysis. The MB agar was at least as sensitive as culturing in selective broth for detection of GBS in vaginal and anorectal swab specimens, and GBS strains could be identified directly on the primary plate due to the CAMP zones without the need for subculturing. The carriage rate of GBS in a group of Danish women was found to be more than 30%, a figure considerably higher than the rate that was reported previously.

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Rapid Identification of Bacteria and Yeast: Summary of a National Committee for Clinical Laboratory Standards Proposed Guideline

Baron

2001

CLIN INFECT DIS

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Using colony morphology on selected agars, Gram-stain morphology, and a number of 1-step biochemical or enzymatic tests, skilled microbiologists can identify the species of the majority of isolates seen routinely in a clinical laboratory. These results are often available more quickly than and are as accurate as those derived from conventional methods. The National Committee for Clinical Laboratory Standards has produced a guideline that describes tests that can be used to identify a number of aerobic gram-negative rods and gram-positive cocci, a number of commonly isolated anaerobes, and 3 species of yeast. An overview of the organisms included in the guideline, the tests that identify them, and the situations in which rapid testing is appropriate is presented here.

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Evaluation of the Spot-CAMP Test for the Rapid Presumptive Identification of Group B Streptococci

Cited by 7 publications

References 0 publications

Evaluation of spot CAMP test for identification of group B streptococci

Evaluation of spot CAMP test for identification of group B streptococci

Method for Quantitative Detection and Presumptive Identification of Group B Streptococci on Primary Plating

Rapid Identification of Bacteria and Yeast: Summary of a National Committee for Clinical Laboratory Standards Proposed Guideline

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