Evaluation of spot CAMP test for identification of group B streptococci

Ratner, Hilda; Weeks, Lyndell S.; Stratton, Charles W.

doi:10.1128/jcm.24.2.296-297.1986

Cited by 12 publications

(2 citation statements)

References 8 publications

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“…To identify GBS isolates, screening with selective enrichment broth is used and the results are known in about 48 h ( Centers for Disease Control and Prevention [CDC], 2010 ). Other methods for GBS identification are as follows: CAMP test ( Ratner et al, 1986 ), serologic test ( Guerrero et al, 2004 ), test on chromogenic agars (non-hemolytic GBS is not detected; Votava et al, 2001 ), or DNA analysis ( Ryan et al, 1999 ; Goodrich and Miller, 2007 ). Rapid diagnostics for carriage/infection in pregnant women, especially those giving birth prematurely, would guarantee immediate implementation of antibiotic prophylaxis/therapy.…”

Section: Introductionmentioning

confidence: 99%

Epitope Mapping of Streptococcus agalactiae Elongation Factor Tu Protein Recognized by Human Sera

et al. 2018

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The elongation factor Tu has been identified as one of the most immunoreactive proteins that was recognized by human sera of GBS (group B streptococcus) positive patients. In this paper, we present the polypeptide-specific epitopes of the bacterial protein that are recognized by human antibodies: 28LTAAITTVLARRLP41 (peptide no. 3) and 294GQVLAKPGSINPHTKF309 (peptide no. 21). To determine the shortest amino acid sequence recognized by antibodies, truncation peptide libraries were prepared using the PEPSCAN method. The analysis of immunoreactivity of peptides with sera of GBS positive and negative women revealed that the most immunoreactive sequence was 306HTKF309. Moreover, we observed that this sequence also showed the highest specificity which was based on ratio of reactivity with sera of GBS positive relative to sera of GBS negative patients. Epitope was synthetized on Wang resin with the Fmoc strategy. Our results open the possibility to use 306HTKF309 peptide in diagnostic assays to determine Streptococcus agalactiae infection in humans.

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Section: Introductionmentioning

confidence: 99%

Epitope Mapping of Streptococcus agalactiae Elongation Factor Tu Protein Recognized by Human Sera

et al. 2018

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“…A total of 91 non-duplicated GBS isolates were systematically collected from vaginal swabs of pregnant women at 35-37 weeks of gestation during the period of January 1 st to December 31 st , 2015. These strains had previously been identified on the basis of Gram staining, colony morphology, β-haemolysis, and a positive CAMP test [20] on blood agar and were further identified by using Vitek 2 Compact system (bioMérieux, Lyon, France). Enterococcus casseliflavus (ATCC 700327) was used as the quality control strain for the GP card.…”

Section: Bacterial Isolatesmentioning

confidence: 99%

Antimicrobial resistance and molecular characterization of Streptococcus agalactiae from pregnant women in southern China

Guo

Peng

et al. 2019

J Infect Dev Ctries

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Introduction: This study aimed to characterize antimicrobial resistance (AMR), molecular determinants of AMR and virulence, as well as clonal relationship of Streptococcus agalactiae isolates from women at 35-37 weeks of gestation in the Chaoshan metropolitan area of southern China. Methodology: Bacterial strains isolated from vaginal swabs were identified and antimicrobial susceptibility tests were performed by using a Vitek 2 Compact system (BioMérieux, France). Resistance and virulence genes were detected by polymerase chain reaction (PCR) and the clonal relationship was analysed by multiple locus variable number tandem repeat analysis (MLVA). Statistical analysis was carried out by using SPSS software, version 19.0. Results: All GBS were susceptible to benzylpenicillin, ampicillin, quinupristin/dalfopristin, tigecycline, linezolid and vancomycin, but a considerable proportion was resistant to clindamycin (29.67%), erythromycin (46.15%)， azithromycin (63.74%), tetracycline (84.62%) and quinolones (25.27%). The carrier rates of ermB (69.04%) and mefA/E (64.28%) were detected in these GBS strains resistant to erythromycin. In terms of MLVA detection, 91 GBS strains were categorized into 43 genotypes and 6 clusters. All GBS harboured hylB and cylE genes, most of which carried a combination of PI-1 and PI-2a genes as a common virulence gene profile. Conclusions: The high level of resistance conferred by some corresponding resistance genes to macrolides, lincosamides and quinolones of GBS isolates from pregnant women in southern China, has reinforced the necessity for monitoring GBS strain resistance to the above agents. Comparative genetic studies of GBS isolates, especially efforts to understand the relationship between pilus islands and genotype, were essential for conducting infection control and epidemiological comparisons between countries.

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