Evaluation of the Sensitivity of an in vitro High Frequency Ultrasound Device to Monitor the Coagulation Process: Study of the Effects of Heparin Treatment in a Murine Model

Callé, Rachel; Rochefort, Gaël Y.; Desbuards, Nicolas; Plag, Camille; Antier, Daniel; Ossant, F.

doi:10.1016/j.ultrasmedbio.2009.10.010

Cited by 6 publications

(13 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An increase in the acoustic velocity of between 30 and 40 m/s was observed. The Figure 2.9: Temporal changes in the US velocity are shown for (A) two volunteers, as reported by Ossant et al [66], and (B) a human and a rat sample as reported by the same group [16].…”

Section: Whole Bloodmentioning

confidence: 78%

See 1 more Smart Citation

Investigating the Kinetics of Blood Coagulation Using High-Frequency Ultrasound

Maher¹

2021

Preprint

View full text Add to dashboard Cite

Blood circulation requires regulated clot formation and breakdown to prevent blood loss following an injury and to ensure that clots do not form and circulate within the vasculature. Known as hemostasis, this delicate balance between coagulant and anti-coagulant pathways can be disrupted by disease, medication, or trauma, and may lead to morbidity or mortality. Current in vitro hemostatic tests have shown promise as tools for diagnosis and risk assessment in certain disorders. However, these tests are limited in their ability to assess the complete hemostatic process or are restricted to studies of blood plasma. In this work, high frequency ultrasound is proposed as a method of assessing hemostasis in whole blood samples. A system was developed and experiments were performed by monitoring acoustic changes in mouse blood during coagulation. Blood cell motion and frequency dependant changes in ultrasound intensity were found to be sensitive to the kinetics of clot formation

show abstract

Section: Whole Bloodmentioning

confidence: 78%

“…It has been reported that the sedimentation of RBCs (or settling of RBC due to gravity) affects the acoustic properties of blood [74]. Increases in the sound speed due to sedimentation were approximately linear with time and resulted in an overall change on the order of 10 m/s after a period of 90 minutes [15,16,53,66].…”

Section: Whole Bloodmentioning

confidence: 99%

Investigating the Kinetics of Blood Coagulation Using High-Frequency Ultrasound

Maher¹

2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Voleišis et al [40] and Huang et al [22] also measured the sound velocity in human and porcine whole blood during clotting, respectively. The velocity of high-frequency sound has also been measured for human and rat blood [30,31]. General speaking, the sound velocity increases gradually during blood coagulation, as shown in Fig.…”

Section: Sound Velocitymentioning

confidence: 98%

“…The results showed that it is possible to temporally divide the blood coagulation process into several stages. The effects of heparin treatment on blood coagulation were also determined in a murine model using the same high-frequency ultrasound device [31].…”

Section: Ultrasound Backscattermentioning

confidence: 99%

“…The high-frequency ultrasound-attenuation coefficient for human blood plasma during clotting was measured for frequencies ranging from 8 to 22 MHz [36]. In order to explore the effect of heparin treatment on blood coagulation, the 10-to 30-MHz high-frequency attenuation coefficient was measured for both human and rat whole blood during coagulation [31].…”

Section: Ultrasound Attenuationmentioning

confidence: 99%

See 1 more Smart Citation

Untitled

Huang¹

2011

J. Med. Biol. Eng.

View full text Add to dashboard Cite

This paper provides a detailed review of the in vitro, in vivo, and clinical applications of ultrasound to understand blood coagulation. The paper focuses on the effect of blood rheology on clotting mechanisms, especially on the use of ultrasound to detect the viscoelastic properties of blood clots. After a short introduction, the paper describes how quantitative ultrasound parameters can be used to study the process of blood coagulation. Ultrasound parameters can be used for tissue characterization, and so the process of blood coagulation can be monitored continuously by measuring the ultrasound backscatter, attenuation coefficient, and velocity. Furthermore, several ultrasound elastography methods including quasi-static strain imaging, shear wave, and acoustic-radiation-force imaging are introduced for measuring the viscoelastic properties of blood clots. The advantages and drawbacks of these ultrasound-based approaches for detecting blood coagulation are discussed. Finally, new areas of research, the clinical application of ultrasound, and areas of potential future developments are presented.

show abstract