Evaluation of the seeds of selected lines of three Lathyrus spp for β‐N‐oxalylamino‐L‐ alanine (BOAA), tannins, trypsin inhibitor activity and certain in‐vitro characteristics

Abstract

Sixty eight lines comprising 36 Lathyrus satiuus, 16 L cicera and 16 of L ochrus were analysed for crude protein (CP), the neurotoxin P-N-oxalylamino-L-alanine (BOAA), protein precipitable tannin (PPT), catechin equivalents (CE), and trypsin inhibitor activity (TIA). Randomly chosen lines from each of the species were also evaluated for other chemical and in-vitro characteristics including neutral detergent fibre (NDF), acid detergent fibre (ADF), in-vitro dry matter and in-vitro organic matter digestibilities (IVDMD, IVOMD), respectively.The species with the lowest and highest CP were L cicera (295.39 f 18.69 g kg-' DM) and L satiuus (324.99 f 13.40 g kg-' DM), respectively. BOAA concentration, also, was least in L cicera (1.26 k 0.18 g kg-' DM) and highest in L satiuus (5.63 k 0.43 g kg-' DM). There were higher intraspecies variations in BOAA levels among L satiuus and L cicera than in L ochus genotypes. On average, BOAA levels in L ochrus and L satiuus were about four to five times higher than those in L cicera. The PPT level in L sativus (4.54 & 0.31 g kg-' DM) was similar to that in the other species. However, there were some lines without detectable PPT in all the species. CE, which detects simple flavonoids as well as condensed tannins, was no more than 6.10 g kg-' DM in any accession, except in L ochrus in which CE ranged from 14.07 g kg-' to 28.50 g kg-' DM. There were high variabilities between and within species in CE values as shown by the high coefficients of variation. Mean TIA in all the L satiuus lines (18.16 f 2.38 g kg-' DM) was similar to that in L ochrus and about twice the levels in L cicera. The coefficients of variation ranged from 10.93% in L ochrus to 17.13% in L satiuus. The ash, organic matter and NDF content of the lines did not differ significantly. However, the ADF, IVDMD and IVOMD differed significantly ( P < 0.05) between the species. The IVDMD and IVOMD of L cicera (88.55 f 0.80% and 85.53 0.85%, respectively) were significantly lower ( P < 0.05) than in the other species. Seed weight correlated significantly ( P < 0.05) with CE and TIA in both L satiuus and L ochrus while C P correlated significatly ( P < 0.01) with TIA in both L satiuus and L cicera with respective r values of 0.70 and -0.75.

Variability of phenotypic and morphological characteristics of some Lathyrus sativus L. and Lathyrus cicera L. accessions and nutritional traits of their seeds

“…Content of this nutrient in red pea, proved to be lower by nearly 5 percent units and, therefore, similar to the results obtained in Franco Jubete (1991) and Hanbury (2000) studies but lower than that reported by Aletor et al (1994). desirable features make them very attractive to produce (Campbell 1997).…”

“…Contents of b-ODAP in seeds of the L. cicera accessions presented in this work (from 911 to 1,344 mg, with average 1,168 mg kg -1 DM) were similar to those from the studies carried out by Aletor et al (1994), but lower than that achieved by Hanbury et al (1999).…”

β-N-oxalyl-L-2,3-diaminopropionic acid, L-homoarginine, and asparagine contents in the seeds of different genotypes Lathyrus sativus L. as determined by UHPLC-MS/MS

“…Homoarginine, a non-protein amino acid, was the first to be characterized from L. sativus seeds but had been ignored because of its lack of toxic attributes. [12,14,15] Homoarginine is now recognized as a normal metabolite in humans and its role in human health is becoming increasingly important. Homoarginine in L. sativus contributes to cardiovascular health.…”

Nutrient constituents, functional attributes andin vitroprotein digestibility of the seeds of theLathyrusplant

“…The genus Lathyrus is classified under the family leguminosae which includes about 150 species distributed within the West Africa and North Africa regions (Aletor et al [2]). The seeds may be eaten raw as snacks or the flour used to adulterate the more expensive flours.…”

“…lt is often referred to as "insurance crop" as it produces reliable yields when all other crops fail especially during drought-induced famine. The major limitations to their full utilization as food is, however, the presence of the potent, low-molecular weight neurotoxin named either β-Noxalyamino-L-alanine (BOAA) or β-N-oxaly-α, β-diaminopropionic acid (ODAP) (Aletor et al [2]). This non-protein has been implicated in the development of the neurodegenerative condition -Lathyrism -in humans [2,3].…”