Evaluation of the remineralising capacities of modified saliva substitutes in vitro

Tschoppe, Peter; Kielbassa, Andrej M.; Meyer-Lueckel, Hendrik

doi:10.1016/j.archoralbio.2009.06.004

Cited by 14 publications

(22 citation statements)

References 22 publications

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“…Although this type of dentin lesion was used before [ten Cate et al, 1995;Tschoppe et al, 2009;Lippert et al, 2015], the protocols for initial demineralization differed (slightly). Methyl cellulose gel [ten Cate et al, 1995], acetic acid solution without fluoride [Lippert et al, 2015], or acetic acid solution with small amounts of fluoride were used [Tschoppe et al, 2009;present data]. Therefore, it might be speculated that the different demineralization protocols resulted in slightly different subsurface dentin lesions [Moron et al, 2013] and, thus, different diffusion properties (for both fluoride and acids).…”

Section: Effects Of Fluoride Varnishesmentioning

confidence: 99%

“…The last third remained uncovered (demineralized treatment area [DT]). To create subsurface artificial dentin caries lesions in the uncovered areas, the specimens were stored in a demineralizing solution for 7 days (2.0 mL solution/mm 2 dentin surface) [Tschoppe et [Tschoppe et al, 2009]. During that period, the pH was monitored daily and, if necessary, adjusted with small amounts of either 10% HCl or 10 M KOH to maintain a constant pH value.…”

Section: Lesion Formationmentioning

confidence: 99%

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Caries-Preventive Effect of NaF, NaF plus TCP, NaF plus CPP-ACP, and SDF Varnishes on Sound Dentin and Artificial Dentin Caries in vitro

Wierichs

Stausberg²,

Lausch³

et al. 2018

Caries Res

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The aim of this study was to compare the caries-preventive effect of different fluoride varnishes on sound dentin as well as on artificial dentin caries-like lesions. Bovine dentin specimens (n = 220) with one sound surface (ST) and one artificial caries lesion (DT) were prepared and randomly allocated to 11 groups. The interventions before pH cycling were as follows: application of a varnish containing NaF (22,600 ppm F^-; Duraphat [NaF₀/NaF₁]), NaF plus tricalcium phosphate (22,600 ppm F^-; Clinpro White Varnish Mint [TCP₀/TCP₁]), NaF plus casein phosphopeptide-stabilized amorphous calcium phosphate complexes (CPP-ACP; 22,600 ppm F^-; MI Varnish [CPP₀/CPP₁]), or silver diamine fluoride (SDF; 35,400 ppm F^-; Cariestop 30% [SDF₀/SDF₁]) and no intervention (N_NB/N₀/N₁). During pH cycling (14 days, 6 × 120 min demineralization/day) half of the specimens in each group were brushed (10 s; 2 times/day) with either fluoride-free (“0”; e.g., TCP₀) or 1,100 ppm F^- (“1”; e.g., TCP₁) dentifrice slurry. In another subgroup, the specimens were pH cycled but not brushed (N_NB). Differences in integrated mineral loss (ΔΔZ), lesion depth (ΔLD), and colorimetric values (ΔΔE) were calculated between the values after initial demineralization and those after pH cycling, using transversal microradiography and photographic images. After pH cycling, no discoloration could be observed. Furthermore, N_NB, N₀, and N₁ showed significantly increased ΔZ_DT/LD_DT and ΔZ_ST/LD_ST values, indicating further demineralization. In contrast, CPP₀, CPP₁, SDF₀, and SDF₁ showed significantly decreased ΔZ_DT/LD_DT values, indicating remineralization (p ≤ 0.004; paired t test). CPP₀, CPP₁, SDF₀, and SDF₁ showed significantly higher changes in ΔΔZ_DT/ΔLD_DT and ΔΔZ_ST/ΔLD_ST than N_NB, N₀, and N₁ (p < 0.001; Bonferroni post hoc test). In conclusion, under the conditions chosen, all fluoride varnishes prevented further demineralization. However, only NaF plus CPP-ACP and SDF could remineralize artificial dentin caries-like lesions under net-demineralizing conditions, thereby indicating that NaF plus CPP-ACP and SDF may be helpful to high-caries-risk patients.

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Section: Effects Of Fluoride Varnishesmentioning

confidence: 99%

Section: Lesion Formationmentioning

confidence: 99%

Caries-Preventive Effect of NaF, NaF plus TCP, NaF plus CPP-ACP, and SDF Varnishes on Sound Dentin and Artificial Dentin Caries in vitro

Wierichs

Stausberg²,

Lausch³

et al. 2018

Caries Res

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“…The calcium-to-phosphate ratio of the modified SN (polysaccharide-based) was determined to be 1: 1.6. The natural calcium and phosphate of the unmodified SN solution [Tschoppe et al, 2009a] and the further addition of calcium (as CaCl 2 ؒ 2H 2 O) and phosphate (as K 2 HPO 4 /KH 2 PO 4 ) resulted in a supersaturated solution with respect to the relevant calcium phosphates at the buffered pH of 5.95 ( table 1 ). Preliminary tests revealed that a K 2 HPO 4 /KH 2 PO 4 ratio (phosphate buffer) of 1: 2 was optimal for maintaining a constant pH (all chemicals were from Merck).…”

Section: Preparation Of Experimental Solutionsmentioning

confidence: 99%

Saliva Substitutes in Combination with Highly Concentrated Fluorides and Brushing: In vitro Effects on Enamel Subsurface Lesions

Tschoppe

Siegel²,

Meyer-Lueckel³

2010

Caries Res

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Hyposalivation is often associated with high caries activity, in particular in patients undergoing irradiation in the head/neck area. Besides the use of saliva substitutes to relieve the oral symptoms, daily application of fluoride gels or toothpaste (5,000 µg F^–/g) is recommended for caries prevention. The aim of this study was to evaluate potentially remineralising effects of these fluoride agents in combination with saliva substitutes on enamel subsurface lesions. Demineralised bovine specimens were either stored in mineral water [control; saturation with respect to octacalcium phosphate (S_OCP): 0.8], a demineralising saliva substitute (Glandosane; S_OCP: 0.3) or in a modified (with respect to S_OCP) saliva substitute [Saliva natura (SN); S_OCP: 1.9] for 5 weeks (37°C). The following treatments were applied twice daily (11–13/group): no treatment (0), ProSchmelz fluoride gel (PS; 10 min application), Duraphat toothpaste (DP; 10 s; brushing with toothpaste/storage solution slurry), combination of DP+PS. Mineral parameters before/after storage were evaluated from microradiographs. Storage in Glandosane led to significant demineralisation (p < 0.05; paired t test), whereas additional use of fluoride agents neutralised the demineralising effect (p > 0.05). Storage in water alone resulted in no changes in mineral parameters (p > 0.05), whereas in combination with fluorides remineralisation could be shown (p < 0.05). For SN alone, remineralisation was observed (p < 0.05), but no additional beneficial effects of fluorides were detected. Under the conditions chosen, the fluoride agents reduce the demineralising effects of Glandosane and promote the remineralisation of specimens stored in water. Remineralising effects of SN could not be enhanced by the fluorides.

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“…21 The water-based positive control solution (PC; Buskes remineralising solution) was prepared according to a previous study (Table 1). 7 The calcium-tophosphate ratio of the Saliva natura solutions was defined at 1:1.6, while the K 2 HPO 4 /KH 2 PO 4 ratio (phosphate buffer) was set at 1:2; the addition of the respective calcium and phosphate concentrations was determined by calculation of the S OCP (1, 2, 3) of various Saliva natura modifications at pH 6.0 (Table 1; all chemicals from Merck). 7 Additionally, all labmodified solutions contained 0.1 mM fluoride (Fisher Scientific, Ulm, Germany).…”

Section: Evaluation Of Degree Of Saturation and Solution Preparationmentioning

confidence: 99%

“…Remineralisation effects could be achieved with experimentally modified Saliva natura solutions which were slightly supersaturated with respect to octacalcium phosphate (OCP) and dicalcium phosphate dihydrate (DCDP). 6,7 In patients with hyposalivation, in particular after radiation therapy, an increased caries incidence can be observed. 1,8 While in vitro irradiated dentine was not more susceptible to caries than non-irradiated in an in situ study (and caries onset has been attributed to reduced salivary flow rates), 9 caries in irradiated patients often starts on the labial surface at the cervical areas of the teeth.…”

Section: Introductionmentioning

confidence: 99%

Saliva substitute in combination with high-concentrated fluoride toothpaste: Effects on demineralised dentin in vitro

Tschoppe

Zandim

Sampaio

et al. 2010

Journal of Dentistry

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Evaluation of the remineralising capacities of modified saliva substitutes in vitro

Cited by 14 publications

References 22 publications

Caries-Preventive Effect of NaF, NaF plus TCP, NaF plus CPP-ACP, and SDF Varnishes on Sound Dentin and Artificial Dentin Caries in vitro

Caries-Preventive Effect of NaF, NaF plus TCP, NaF plus CPP-ACP, and SDF Varnishes on Sound Dentin and Artificial Dentin Caries in vitro

Saliva Substitutes in Combination with Highly Concentrated Fluorides and Brushing: In vitro Effects on Enamel Subsurface Lesions

Saliva substitute in combination with high-concentrated fluoride toothpaste: Effects on demineralised dentin in vitro

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